Topographic changes in SARS coronavirus-infected cells at late stages of infection.
Identifieur interne : 002A43 ( PubMed/Curation ); précédent : 002A42; suivant : 002A44Topographic changes in SARS coronavirus-infected cells at late stages of infection.
Auteurs : M L Ng [Singapour] ; J W M. Lee ; M L N. Leong ; A-E Ling ; H-C Tan ; E E OoiSource :
- Emerging infectious diseases [ 1080-6040 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- pathogénicité : Virus du SRAS.
- virologie : Cellules Vero, Membrane cellulaire.
- ultrastructure : Animaux, Cellules Vero, Effet cytopathogène viral, Facteurs temps, Membrane cellulaire, Microscopie à force atomique, Microscopie électronique à balayage, Réplication virale.
English descriptors
- KwdEn :
- MESH :
- pathogenicity : SARS Virus.
- ultrastructure : Cell Membrane, Vero Cells.
- virology : Cell Membrane, Vero Cells.
- Animals, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Time Factors, Virus Replication.
Abstract
Scanning electron and atomic force microscopy was used for the first time to view the maturation of the severe acute respiratory syndrome-associated coronavirus at the cell surface. The surface form of the cells at advanced infection displayed prolific pseudopodia that, in addition to the rest of the plasma membrane, were also active sites of virus release. High magnification of the maturing virus particles showed a rosette appearance with short knoblike spikes under both the scanning electron and atomic force microscopes. The final expulsion step of the maturing virus particles seemed to result in some disruptions to the plasma membrane. The cytoskeletal network along the edge of the infected cells was enhanced and could be involved in transporting and expelling the progeny virus particles. Thickening of the actin filaments at the cell edge provided the bending force to extrude the virus particles.
DOI: 10.3201/eid1011.040195
PubMed: 15550199
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Leong</name></author><author><name sortKey="Ling, A E" sort="Ling, A E" uniqKey="Ling A" first="A-E" last="Ling">A-E Ling</name></author><author><name sortKey="Tan, H C" sort="Tan, H C" uniqKey="Tan H" first="H-C" last="Tan">H-C Tan</name></author><author><name sortKey="Ooi, E E" sort="Ooi, E E" uniqKey="Ooi E" first="E E" last="Ooi">E E Ooi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15550199</idno><idno type="pmid">15550199</idno><idno type="doi">10.3201/eid1011.040195</idno><idno type="wicri:Area/PubMed/Corpus">002A43</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A43</idno><idno type="wicri:Area/PubMed/Curation">002A43</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A43</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Topographic changes in SARS coronavirus-infected cells at late stages of infection.</title><author><name sortKey="Ng, M L" sort="Ng, M L" uniqKey="Ng M" first="M L" last="Ng">M L Ng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, National University of Singapore, Singapore. micngml@nus.edu.sg</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, National University of Singapore</wicri:regionArea></affiliation></author><author><name sortKey="Lee, J W M" sort="Lee, J W M" uniqKey="Lee J" first="J W M" last="Lee">J W M. Lee</name></author><author><name sortKey="Leong, M L N" sort="Leong, M L N" uniqKey="Leong M" first="M L N" last="Leong">M L N. Leong</name></author><author><name sortKey="Ling, A E" sort="Ling, A E" uniqKey="Ling A" first="A-E" last="Ling">A-E Ling</name></author><author><name sortKey="Tan, H C" sort="Tan, H C" uniqKey="Tan H" first="H-C" last="Tan">H-C Tan</name></author><author><name sortKey="Ooi, E E" sort="Ooi, E E" uniqKey="Ooi E" first="E E" last="Ooi">E E Ooi</name></author></analytic><series><title level="j">Emerging infectious diseases</title><idno type="ISSN">1080-6040</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Membrane (ultrastructure)</term><term>Cell Membrane (virology)</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Microscopy, Atomic Force</term><term>Microscopy, Electron, Scanning</term><term>SARS Virus (pathogenicity)</term><term>Time Factors</term><term>Vero Cells (ultrastructure)</term><term>Vero Cells (virology)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero (ultrastructure)</term><term>Cellules Vero (virologie)</term><term>Effet cytopathogène viral</term><term>Facteurs temps</term><term>Membrane cellulaire (ultrastructure)</term><term>Membrane cellulaire (virologie)</term><term>Microscopie à force atomique</term><term>Microscopie électronique à balayage</term><term>Réplication virale</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Membrane</term><term>Vero Cells</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules Vero</term><term>Membrane cellulaire</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Membrane</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Microscopy, Atomic Force</term><term>Microscopy, Electron, Scanning</term><term>Time Factors</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Effet cytopathogène viral</term><term>Facteurs temps</term><term>Membrane cellulaire</term><term>Microscopie à force atomique</term><term>Microscopie électronique à balayage</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Scanning electron and atomic force microscopy was used for the first time to view the maturation of the severe acute respiratory syndrome-associated coronavirus at the cell surface. The surface form of the cells at advanced infection displayed prolific pseudopodia that, in addition to the rest of the plasma membrane, were also active sites of virus release. High magnification of the maturing virus particles showed a rosette appearance with short knoblike spikes under both the scanning electron and atomic force microscopes. The final expulsion step of the maturing virus particles seemed to result in some disruptions to the plasma membrane. The cytoskeletal network along the edge of the infected cells was enhanced and could be involved in transporting and expelling the progeny virus particles. Thickening of the actin filaments at the cell edge provided the bending force to extrude the virus particles.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15550199</PMID><DateCompleted><Year>2005</Year><Month>08</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1080-6040</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><Issue>11</Issue><PubDate><Year>2004</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Emerging infectious diseases</Title><ISOAbbreviation>Emerging Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>Topographic changes in SARS coronavirus-infected cells at late stages of infection.</ArticleTitle><Pagination><MedlinePgn>1907-14</MedlinePgn></Pagination><Abstract><AbstractText>Scanning electron and atomic force microscopy was used for the first time to view the maturation of the severe acute respiratory syndrome-associated coronavirus at the cell surface. The surface form of the cells at advanced infection displayed prolific pseudopodia that, in addition to the rest of the plasma membrane, were also active sites of virus release. High magnification of the maturing virus particles showed a rosette appearance with short knoblike spikes under both the scanning electron and atomic force microscopes. The final expulsion step of the maturing virus particles seemed to result in some disruptions to the plasma membrane. The cytoskeletal network along the edge of the infected cells was enhanced and could be involved in transporting and expelling the progeny virus particles. Thickening of the actin filaments at the cell edge provided the bending force to extrude the virus particles.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>M L</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Department of Microbiology, National University of Singapore, Singapore. micngml@nus.edu.sg</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>J W M</ForeName><Initials>JW</Initials></Author><Author ValidYN="Y"><LastName>Leong</LastName><ForeName>M L N</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>Ling</LastName><ForeName>A-E</ForeName><Initials>AE</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>H-C</ForeName><Initials>HC</Initials></Author><Author ValidYN="Y"><LastName>Ooi</LastName><ForeName>E E</ForeName><Initials>EE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType 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