Topographic changes in SARS coronavirus-infected cells at late stages of infection.
Identifieur interne : 002A43 ( PubMed/Corpus ); précédent : 002A42; suivant : 002A44Topographic changes in SARS coronavirus-infected cells at late stages of infection.
Auteurs : M L Ng ; J W M. Lee ; M L N. Leong ; A-E Ling ; H-C Tan ; E E OoiSource :
- Emerging infectious diseases [ 1080-6040 ] ; 2004.
English descriptors
- KwdEn :
- MESH :
- pathogenicity : SARS Virus.
- ultrastructure : Cell Membrane, Vero Cells.
- virology : Cell Membrane, Vero Cells.
- Animals, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Time Factors, Virus Replication.
Abstract
Scanning electron and atomic force microscopy was used for the first time to view the maturation of the severe acute respiratory syndrome-associated coronavirus at the cell surface. The surface form of the cells at advanced infection displayed prolific pseudopodia that, in addition to the rest of the plasma membrane, were also active sites of virus release. High magnification of the maturing virus particles showed a rosette appearance with short knoblike spikes under both the scanning electron and atomic force microscopes. The final expulsion step of the maturing virus particles seemed to result in some disruptions to the plasma membrane. The cytoskeletal network along the edge of the infected cells was enhanced and could be involved in transporting and expelling the progeny virus particles. Thickening of the actin filaments at the cell edge provided the bending force to extrude the virus particles.
DOI: 10.3201/eid1011.040195
PubMed: 15550199
Links to Exploration step
pubmed:15550199Le document en format XML
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<author><name sortKey="Leong, M L N" sort="Leong, M L N" uniqKey="Leong M" first="M L N" last="Leong">M L N. Leong</name>
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<author><name sortKey="Ling, A E" sort="Ling, A E" uniqKey="Ling A" first="A-E" last="Ling">A-E Ling</name>
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<author><name sortKey="Tan, H C" sort="Tan, H C" uniqKey="Tan H" first="H-C" last="Tan">H-C Tan</name>
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<series><title level="j">Emerging infectious diseases</title>
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<term>Cell Membrane (ultrastructure)</term>
<term>Cell Membrane (virology)</term>
<term>Chlorocebus aethiops</term>
<term>Cytopathogenic Effect, Viral</term>
<term>Microscopy, Atomic Force</term>
<term>Microscopy, Electron, Scanning</term>
<term>SARS Virus (pathogenicity)</term>
<term>Time Factors</term>
<term>Vero Cells (ultrastructure)</term>
<term>Vero Cells (virology)</term>
<term>Virus Replication</term>
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<term>Chlorocebus aethiops</term>
<term>Cytopathogenic Effect, Viral</term>
<term>Microscopy, Atomic Force</term>
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<front><div type="abstract" xml:lang="en">Scanning electron and atomic force microscopy was used for the first time to view the maturation of the severe acute respiratory syndrome-associated coronavirus at the cell surface. The surface form of the cells at advanced infection displayed prolific pseudopodia that, in addition to the rest of the plasma membrane, were also active sites of virus release. High magnification of the maturing virus particles showed a rosette appearance with short knoblike spikes under both the scanning electron and atomic force microscopes. The final expulsion step of the maturing virus particles seemed to result in some disruptions to the plasma membrane. The cytoskeletal network along the edge of the infected cells was enhanced and could be involved in transporting and expelling the progeny virus particles. Thickening of the actin filaments at the cell edge provided the bending force to extrude the virus particles.</div>
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<Abstract><AbstractText>Scanning electron and atomic force microscopy was used for the first time to view the maturation of the severe acute respiratory syndrome-associated coronavirus at the cell surface. The surface form of the cells at advanced infection displayed prolific pseudopodia that, in addition to the rest of the plasma membrane, were also active sites of virus release. High magnification of the maturing virus particles showed a rosette appearance with short knoblike spikes under both the scanning electron and atomic force microscopes. The final expulsion step of the maturing virus particles seemed to result in some disruptions to the plasma membrane. The cytoskeletal network along the edge of the infected cells was enhanced and could be involved in transporting and expelling the progeny virus particles. Thickening of the actin filaments at the cell edge provided the bending force to extrude the virus particles.</AbstractText>
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