Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Identifieur interne : 000F99 ( Pmc/Curation ); précédent : 000F98; suivant : 001000Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Auteurs : Victor M. Corman [Allemagne] ; Olfert Landt [Allemagne] ; Marco Kaiser [Allemagne] ; Richard Molenkamp [Pays-Bas] ; Adam Meijer [Pays-Bas] ; Daniel Kw Chu [République populaire de Chine] ; Tobias Bleicker [Allemagne] ; Sebastian Brünink [Allemagne] ; Julia Schneider [Allemagne] ; Marie Luisa Schmidt [Allemagne] ; Daphne Gjc Mulders [Pays-Bas] ; Bart L. Haagmans [Pays-Bas] ; Bas Van Der Veer [Pays-Bas] ; Sharon Van Den Brink [Pays-Bas] ; Lisa Wijsman [Pays-Bas] ; Gabriel Goderski [Pays-Bas] ; Jean-Louis Romette [France] ; Joanna Ellis [Royaume-Uni] ; Maria Zambon [Royaume-Uni] ; Malik Peiris [République populaire de Chine] ; Herman Goossens [Belgique] ; Chantal Reusken [Pays-Bas] ; Marion Pg Koopmans [Pays-Bas] ; Christian Drosten [Allemagne]Source :
- Eurosurveillance [ 1025-496X ] ; 2020.
Abstract
The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.
We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.
Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project.
The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.
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DOI: 10.2807/1560-7917.ES.2020.25.3.2000045
PubMed: 31992387
PubMed Central: 6988269
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Corman</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Landt, Olfert" sort="Landt, Olfert" uniqKey="Landt O" first="Olfert" last="Landt">Olfert Landt</name><affiliation wicri:level="1"><nlm:aff id="aff2">Tib-Molbiol, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Tib-Molbiol, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Kaiser, Marco" sort="Kaiser, Marco" uniqKey="Kaiser M" first="Marco" last="Kaiser">Marco Kaiser</name><affiliation wicri:level="1"><nlm:aff id="aff2">Tib-Molbiol, Berlin, Germany</nlm:aff><country 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Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Brunink, Sebastian" sort="Brunink, Sebastian" uniqKey="Brunink S" first="Sebastian" last="Brünink">Sebastian Brünink</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Schneider, Julia" sort="Schneider, Julia" uniqKey="Schneider J" first="Julia" last="Schneider">Julia Schneider</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Schmidt, Marie Luisa" sort="Schmidt, Marie Luisa" uniqKey="Schmidt M" first="Marie Luisa" last="Schmidt">Marie Luisa Schmidt</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Mulders, Daphne Gjc" sort="Mulders, Daphne Gjc" uniqKey="Mulders D" first="Daphne Gjc" last="Mulders">Daphne Gjc Mulders</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Van Der Veer, Bas" sort="Van Der Veer, Bas" uniqKey="Van Der Veer B" first="Bas" last="Van Der Veer">Bas Van Der Veer</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Van Den Brink, Sharon" sort="Van Den Brink, Sharon" uniqKey="Van Den Brink S" first="Sharon" last="Van Den Brink">Sharon Van Den Brink</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Wijsman, Lisa" sort="Wijsman, Lisa" uniqKey="Wijsman L" first="Lisa" last="Wijsman">Lisa Wijsman</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Goderski, Gabriel" sort="Goderski, Gabriel" uniqKey="Goderski G" first="Gabriel" last="Goderski">Gabriel Goderski</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Romette, Jean Louis" sort="Romette, Jean Louis" uniqKey="Romette J" first="Jean-Louis" last="Romette">Jean-Louis Romette</name><affiliation wicri:level="1"><nlm:aff id="aff6">Universite d Aix-Marseille, Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Universite d Aix-Marseille, Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Ellis, Joanna" sort="Ellis, Joanna" uniqKey="Ellis J" first="Joanna" last="Ellis">Joanna Ellis</name><affiliation wicri:level="1"><nlm:aff id="aff7">Public Health England, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea></affiliation></author><author><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name><affiliation wicri:level="1"><nlm:aff id="aff7">Public Health England, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea></affiliation></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff5">University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" last="Goossens">Herman Goossens</name><affiliation wicri:level="1"><nlm:aff id="aff8">Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp</wicri:regionArea></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Koopmans, Marion Pg" sort="Koopmans, Marion Pg" uniqKey="Koopmans M" first="Marion Pg" last="Koopmans">Marion Pg Koopmans</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31992387</idno><idno type="pmc">6988269</idno><idno 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Corman</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Landt, Olfert" sort="Landt, Olfert" uniqKey="Landt O" first="Olfert" last="Landt">Olfert Landt</name><affiliation wicri:level="1"><nlm:aff id="aff2">Tib-Molbiol, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Tib-Molbiol, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Kaiser, Marco" sort="Kaiser, Marco" uniqKey="Kaiser M" first="Marco" last="Kaiser">Marco Kaiser</name><affiliation wicri:level="1"><nlm:aff id="aff2">Tib-Molbiol, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Tib-Molbiol, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Molenkamp, Richard" sort="Molenkamp, Richard" uniqKey="Molenkamp R" first="Richard" last="Molenkamp">Richard Molenkamp</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Meijer, Adam" sort="Meijer, Adam" uniqKey="Meijer A" first="Adam" last="Meijer">Adam Meijer</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Chu, Daniel Kw" sort="Chu, Daniel Kw" uniqKey="Chu D" first="Daniel Kw" last="Chu">Daniel Kw Chu</name><affiliation wicri:level="1"><nlm:aff id="aff5">University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Bleicker, Tobias" sort="Bleicker, Tobias" uniqKey="Bleicker T" first="Tobias" last="Bleicker">Tobias Bleicker</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Brunink, Sebastian" sort="Brunink, Sebastian" uniqKey="Brunink S" first="Sebastian" last="Brünink">Sebastian Brünink</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Schneider, Julia" sort="Schneider, Julia" uniqKey="Schneider J" first="Julia" last="Schneider">Julia Schneider</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Schmidt, Marie Luisa" sort="Schmidt, Marie Luisa" uniqKey="Schmidt M" first="Marie Luisa" last="Schmidt">Marie Luisa Schmidt</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Mulders, Daphne Gjc" sort="Mulders, Daphne Gjc" uniqKey="Mulders D" first="Daphne Gjc" last="Mulders">Daphne Gjc Mulders</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Van Der Veer, Bas" sort="Van Der Veer, Bas" uniqKey="Van Der Veer B" first="Bas" last="Van Der Veer">Bas Van Der Veer</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Van Den Brink, Sharon" sort="Van Den Brink, Sharon" uniqKey="Van Den Brink S" first="Sharon" last="Van Den Brink">Sharon Van Den Brink</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Wijsman, Lisa" sort="Wijsman, Lisa" uniqKey="Wijsman L" first="Lisa" last="Wijsman">Lisa Wijsman</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Goderski, Gabriel" sort="Goderski, Gabriel" uniqKey="Goderski G" first="Gabriel" last="Goderski">Gabriel Goderski</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Romette, Jean Louis" sort="Romette, Jean Louis" uniqKey="Romette J" first="Jean-Louis" last="Romette">Jean-Louis Romette</name><affiliation wicri:level="1"><nlm:aff id="aff6">Universite d Aix-Marseille, Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Universite d Aix-Marseille, Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Ellis, Joanna" sort="Ellis, Joanna" uniqKey="Ellis J" first="Joanna" last="Ellis">Joanna Ellis</name><affiliation wicri:level="1"><nlm:aff id="aff7">Public Health England, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea></affiliation></author><author><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name><affiliation wicri:level="1"><nlm:aff id="aff7">Public Health England, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea></affiliation></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff5">University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" last="Goossens">Herman Goossens</name><affiliation wicri:level="1"><nlm:aff id="aff8">Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp</wicri:regionArea></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Koopmans, Marion Pg" sort="Koopmans, Marion Pg" uniqKey="Koopmans M" first="Marion Pg" last="Koopmans">Marion Pg Koopmans</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="1"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author></analytic><series><title level="j">Eurosurveillance</title><idno type="ISSN">1025-496X</idno><idno type="eISSN">1560-7917</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.</p></sec><sec><title>Aim</title><p>We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.</p></sec><sec><title>Methods</title><p>Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.</p></sec><sec><title>Results</title><p>The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project.</p></sec><sec><title>Conclusion</title><p>The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Stohr, K" uniqKey="Stohr K">K Stöhr</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Abbott, A" uniqKey="Abbott A">A Abbott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Müller</name></author><author><name sortKey="Costabel, U" uniqKey="Costabel U">U Costabel</name></author><author><name sortKey="Timm, J" uniqKey="Timm J">J Timm</name></author><author><name sortKey="Binger, T" uniqKey="Binger T">T Binger</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Günther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S van der Werf</name></author><author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S Becker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M Eickmann</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T Bleicker</name></author><author><name sortKey="Brunink, S" uniqKey="Brunink S">S Brünink</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M Eschbach-Bludau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I Eckerle</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T Bleicker</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A Zaki</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M Eschbach-Bludau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name></author><author><name sortKey="Charrel, Rn" uniqKey="Charrel R">RN Charrel</name></author><author><name sortKey="Donoso Mantke, O" uniqKey="Donoso Mantke O">O Donoso Mantke</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name></author><author><name sortKey="Niedrig, M" uniqKey="Niedrig M">M Niedrig</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Rasche, A" uniqKey="Rasche A">A Rasche</name></author><author><name sortKey="Baronti, C" uniqKey="Baronti C">C Baronti</name></author><author><name sortKey="Aldabbagh, S" uniqKey="Aldabbagh S">S Aldabbagh</name></author><author><name sortKey="Cadar, D" uniqKey="Cadar D">D Cadar</name></author><author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name></author><author><name sortKey="Gloza Rausch, F" uniqKey="Gloza Rausch F">F Gloza-Rausch</name></author><author><name sortKey="Glende, J" uniqKey="Glende J">J Glende</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name></author><author><name sortKey="Goettsche, M" uniqKey="Goettsche M">M Goettsche</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Roth, H" uniqKey="Roth H">H Roth</name></author><author><name sortKey="Binger, T" uniqKey="Binger T">T Binger</name></author><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R Dijkman</name></author><author><name sortKey="Gottula, Lt" uniqKey="Gottula L">LT Gottula</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name></author><author><name sortKey="Niemeyer, D" uniqKey="Niemeyer D">D Niemeyer</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cui, J" uniqKey="Cui J">J Cui</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romette, Jl" uniqKey="Romette J">JL Romette</name></author><author><name sortKey="Prat, Cm" uniqKey="Prat C">CM Prat</name></author><author><name sortKey="Gould, Ea" uniqKey="Gould E">EA Gould</name></author><author><name sortKey="De Lamballerie, X" uniqKey="De Lamballerie X">X de Lamballerie</name></author><author><name sortKey="Charrel, R" uniqKey="Charrel R">R Charrel</name></author><author><name sortKey="Coutard, B" uniqKey="Coutard B">B Coutard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alleweldt, F" uniqKey="Alleweldt F">F Alleweldt</name></author><author><name sortKey="Kara, S" uniqKey="Kara S">S Kara</name></author><author><name sortKey="Osinski, A" uniqKey="Osinski A">A Osinski</name></author><author><name sortKey="Van Baal, P" uniqKey="Van Baal P">P Van Baal</name></author><author><name sortKey="Kellerborg, K" uniqKey="Kellerborg K">K Kellerborg</name></author><author><name sortKey="Aarestrup, Fm" uniqKey="Aarestrup F">FM Aarestrup</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Domingo, C" uniqKey="Domingo C">C Domingo</name></author><author><name sortKey="Ellerbrok, H" uniqKey="Ellerbrok H">H Ellerbrok</name></author><author><name sortKey="Koopmans, M" uniqKey="Koopmans M">M Koopmans</name></author><author><name sortKey="Nitsche, A" uniqKey="Nitsche A">A Nitsche</name></author><author><name sortKey="Leitmeyer, K" uniqKey="Leitmeyer K">K Leitmeyer</name></author><author><name sortKey="Charrel, Rn" uniqKey="Charrel R">RN Charrel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pas, Sd" uniqKey="Pas S">SD Pas</name></author><author><name sortKey="Patel, P" uniqKey="Patel P">P Patel</name></author><author><name sortKey="Reusken, C" uniqKey="Reusken C">C Reusken</name></author><author><name sortKey="Domingo, C" uniqKey="Domingo C">C Domingo</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gobat, N" uniqKey="Gobat N">N Gobat</name></author><author><name sortKey="Amuasi, J" uniqKey="Amuasi J">J Amuasi</name></author><author><name sortKey="Yazdanpanah, Y" uniqKey="Yazdanpanah Y">Y Yazdanpanah</name></author><author><name sortKey="Sigfid, L" uniqKey="Sigfid L">L Sigfid</name></author><author><name sortKey="Davies, H" uniqKey="Davies H">H Davies</name></author><author><name sortKey="Byrne, Jp" uniqKey="Byrne J">JP Byrne</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Euro Surveill</journal-id><journal-id journal-id-type="iso-abbrev">Euro Surveill</journal-id><journal-id journal-id-type="publisher-id">eurosurveillance</journal-id><journal-title-group><journal-title>Eurosurveillance</journal-title></journal-title-group><issn pub-type="ppub">1025-496X</issn><issn pub-type="epub">1560-7917</issn><publisher><publisher-name>European Centre for Disease Prevention and Control (ECDC)</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31992387</article-id><article-id pub-id-type="pmc">6988269</article-id><article-id pub-id-type="publisher-id">2000045</article-id><article-id pub-id-type="doi">10.2807/1560-7917.ES.2020.25.3.2000045</article-id><article-id pub-id-type="publisher-id">2000045</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group></article-categories><title-group><article-title>Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Corman</surname><given-names>Victor M</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Landt</surname><given-names>Olfert</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kaiser</surname><given-names>Marco</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Molenkamp</surname><given-names>Richard</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Meijer</surname><given-names>Adam</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chu</surname><given-names>Daniel KW</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bleicker</surname><given-names>Tobias</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Brünink</surname><given-names>Sebastian</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Schneider</surname><given-names>Julia</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Schmidt</surname><given-names>Marie Luisa</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mulders</surname><given-names>Daphne GJC</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Haagmans</surname><given-names>Bart L</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>van der Veer</surname><given-names>Bas</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>van den Brink</surname><given-names>Sharon</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wijsman</surname><given-names>Lisa</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Goderski</surname><given-names>Gabriel</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Romette</surname><given-names>Jean-Louis</given-names></name><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ellis</surname><given-names>Joanna</given-names></name><xref ref-type="aff" rid="aff7"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zambon</surname><given-names>Maria</given-names></name><xref ref-type="aff" rid="aff7"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>Peiris</surname><given-names>Malik</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Goossens</surname><given-names>Herman</given-names></name><xref ref-type="aff" rid="aff8"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Reusken</surname><given-names>Chantal</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Koopmans</surname><given-names>Marion PG</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Drosten</surname><given-names>Christian</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><aff id="aff1"><label>1</label>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</aff><aff id="aff2"><label>2</label>Tib-Molbiol, Berlin, Germany</aff><aff id="aff3"><label>3</label>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</aff><aff id="aff4"><label>4</label>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</aff><aff id="aff5"><label>5</label>University of Hong Kong, Hong Kong, China</aff><aff id="aff6"><label>6</label>Universite d Aix-Marseille, Marseille, France</aff><aff id="aff7"><label>7</label>Public Health England, London, United Kingdom</aff><aff id="aff8"><label>8</label>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium</aff></contrib-group><author-notes><fn id="afn1"><p>Correspondence: Christian Drosten (<email xlink:href="christian.drosten@charite.de">christian.drosten@charite.de</email>)</p></fn></author-notes><pub-date pub-type="ppub"><day>23</day><month>1</month><year>2020</year></pub-date><volume>25</volume><issue>3</issue><elocation-id>2000045</elocation-id><history><date date-type="received"><day>21</day><month>1</month><year>2020</year></date><date date-type="accepted"><day>22</day><month>1</month><year>2020</year></date></history><permissions><copyright-statement>This article is copyright of the authors or their affiliated institutions, 2020.</copyright-statement><copyright-year>2020</copyright-year><copyright-holder>The authors or their affiliated institutions</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.</license-p></license></permissions><abstract><sec><title>Background</title><p>The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.</p></sec><sec><title>Aim</title><p>We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.</p></sec><sec><title>Methods</title><p>Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.</p></sec><sec><title>Results</title><p>The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project.</p></sec><sec><title>Conclusion</title><p>The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.</p></sec></abstract><kwd-group><title>Keywords: </title><kwd>novel coronavirus</kwd><kwd>RT-PCR</kwd><kwd>testing</kwd><kwd>laboratory</kwd><kwd>diagnostics</kwd><kwd>Wuhan</kwd><kwd>2019-nCoV</kwd><kwd>outbreak</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>sequence</meta-name><meta-value>5</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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