Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infection
Identifieur interne : 000961 ( PascalFrancis/Curation ); précédent : 000960; suivant : 000962Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infection
Auteurs : Fang-Ying Liang [Taïwan] ; Leng-Chieh Lin [Taïwan] ; Tsung-Ho Ying [Taïwan] ; Chen-Wen Yao [Taïwan] ; Tswen-Kei Tang [Taïwan] ; Yi-Wen Chen [Taïwan] ; Ming-Hon Hou [Taïwan]Source :
- Journal of virological methods [ 0166-0934 ] ; 2013.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
Previous studies have reported that a prokaryotic-expressed recombinant nucleocapsid protein (NP) is a suitable reagent for the epidemiological screening of coronavirus infection. In this study, soluble recombinant human coronavirus OC43 (HCoV-OC43) NP was produced to examine the antigenicity of the HCoV-OC43 NP of betacoronavirus. Using the purified recombinant NP as an antigen, a polyclonal antibody from rabbit serum with specificity for HCoV-OC43 NP was generated; this antibody reacts specifically with HCoV-OC43 NP and does not cross-react with other human CoV NPs (including those of SARS-CoV and HCoV-229E) by Western blot. Sera from 26 young adults, 17 middle-aged and elderly patients with respiratory infection, and 15 cord blood samples were also tested. Strong reactivity to the NPs of HCoV-OC43 was observed in 96%, 82%, and 93% of the serum samples from the young adults, respiratory patients, and cord blood samples, respectively. To identify the immunoreactivities of the three structural regions of the NP that are recognised by the rabbit polyclonal antibody and human serum, the antigenicities of three protein fragments, including the N-terminal domain (aa 1-173), the central-linker region (aa 174-300), and the C-terminal domain (aa 301-448), were evaluated by Western blot. The rabbit polyclonal antibody demonstrated greater immunoreactivity to the central-linker region and the C-terminal domain than to the N-terminal domain. Three different patterns for the immunoreactivities of the three structural regions of HCoV-OC43 NP were observed in human serum, suggesting variability in the immune responses that occur during HCoV-OC43 infection in humans. The central-linker region of the NP appeared to be the most highly immunoreactive region for all three patterns observed. The goal of this study was to offer insight into the design of diagnostic tools for HCoV infection.
| pA |
|
|---|
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000026
Links to Exploration step
Pascal:13-0084742Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infection</title><author><name sortKey="Liang, Fang Ying" sort="Liang, Fang Ying" uniqKey="Liang F" first="Fang-Ying" last="Liang">Fang-Ying Liang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biotechnology Center, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>1 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Lin, Leng Chieh" sort="Lin, Leng Chieh" uniqKey="Lin L" first="Leng-Chieh" last="Lin">Leng-Chieh Lin</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Emergency Medicine, Chang Gung Memorial Hospital</s1><s2>Puzih City, Chiayi</s2><s3>TWN</s3><sZ>2 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Departments of Nursing and Respiratory Care, Chang Gung Institute of Technology</s1><s2>Puzih City, Chiayi</s2><s3>TWN</s3><sZ>2 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Ying, Tsung Ho" sort="Ying, Tsung Ho" uniqKey="Ying T" first="Tsung-Ho" last="Ying">Tsung-Ho Ying</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Obstetrics and Gynaecology, School of Medicine, College of Medicine, Chung Shan Medical University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>3 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Yao, Chen Wen" sort="Yao, Chen Wen" uniqKey="Yao C" first="Chen-Wen" last="Yao">Chen-Wen Yao</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Pathology, Tri-Service General Hospital, National Defense University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Tang, Tswen Kei" sort="Tang, Tswen Kei" uniqKey="Tang T" first="Tswen-Kei" last="Tang">Tswen-Kei Tang</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medical Laboratory Science and Biotechnology, Yuanpei University</s1><s2>Hsinchu</s2><s3>TWN</s3><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chen, Yi Wen" sort="Chen, Yi Wen" uniqKey="Chen Y" first="Yi-Wen" last="Chen">Yi-Wen Chen</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Pathology, Tri-Service General Hospital, National Defense University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Hou, Ming Hon" sort="Hou, Ming Hon" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biotechnology Center, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>College of Life Science, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="09"><s1>Institute of Genomics and Bioinformatics, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">13-0084742</idno><date when="2013">2013</date><idno type="stanalyst">PASCAL 13-0084742 INIST</idno><idno type="RBID">Pascal:13-0084742</idno><idno type="wicri:Area/PascalFrancis/Corpus">000026</idno><idno type="wicri:Area/PascalFrancis/Curation">000961</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infection</title><author><name sortKey="Liang, Fang Ying" sort="Liang, Fang Ying" uniqKey="Liang F" first="Fang-Ying" last="Liang">Fang-Ying Liang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biotechnology Center, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>1 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Lin, Leng Chieh" sort="Lin, Leng Chieh" uniqKey="Lin L" first="Leng-Chieh" last="Lin">Leng-Chieh Lin</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Emergency Medicine, Chang Gung Memorial Hospital</s1><s2>Puzih City, Chiayi</s2><s3>TWN</s3><sZ>2 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Departments of Nursing and Respiratory Care, Chang Gung Institute of Technology</s1><s2>Puzih City, Chiayi</s2><s3>TWN</s3><sZ>2 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Ying, Tsung Ho" sort="Ying, Tsung Ho" uniqKey="Ying T" first="Tsung-Ho" last="Ying">Tsung-Ho Ying</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Obstetrics and Gynaecology, School of Medicine, College of Medicine, Chung Shan Medical University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>3 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Yao, Chen Wen" sort="Yao, Chen Wen" uniqKey="Yao C" first="Chen-Wen" last="Yao">Chen-Wen Yao</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Pathology, Tri-Service General Hospital, National Defense University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Tang, Tswen Kei" sort="Tang, Tswen Kei" uniqKey="Tang T" first="Tswen-Kei" last="Tang">Tswen-Kei Tang</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Medical Laboratory Science and Biotechnology, Yuanpei University</s1><s2>Hsinchu</s2><s3>TWN</s3><sZ>5 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chen, Yi Wen" sort="Chen, Yi Wen" uniqKey="Chen Y" first="Yi-Wen" last="Chen">Yi-Wen Chen</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Pathology, Tri-Service General Hospital, National Defense University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Hou, Ming Hon" sort="Hou, Ming Hon" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Biotechnology Center, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>College of Life Science, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="09"><s1>Institute of Genomics and Bioinformatics, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author></analytic><series><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term><term>Antigenicity</term><term>Diagnosis</term><term>Human</term><term>Human coronavirus OC43</term><term>Immunoblotting assay</term><term>Nucleocapsid</term><term>Protein</term><term>Strain</term><term>Viral disease</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus OC43 humain</term><term>Homme</term><term>Nucléocapside</term><term>Protéine</term><term>Méthode immunoblotting</term><term>Diagnostic</term><term>Souche</term><term>Antigénicité</term><term>Anticorps</term><term>Virose</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Previous studies have reported that a prokaryotic-expressed recombinant nucleocapsid protein (NP) is a suitable reagent for the epidemiological screening of coronavirus infection. In this study, soluble recombinant human coronavirus OC43 (HCoV-OC43) NP was produced to examine the antigenicity of the HCoV-OC43 NP of betacoronavirus. Using the purified recombinant NP as an antigen, a polyclonal antibody from rabbit serum with specificity for HCoV-OC43 NP was generated; this antibody reacts specifically with HCoV-OC43 NP and does not cross-react with other human CoV NPs (including those of SARS-CoV and HCoV-229E) by Western blot. Sera from 26 young adults, 17 middle-aged and elderly patients with respiratory infection, and 15 cord blood samples were also tested. Strong reactivity to the NPs of HCoV-OC43 was observed in 96%, 82%, and 93% of the serum samples from the young adults, respiratory patients, and cord blood samples, respectively. To identify the immunoreactivities of the three structural regions of the NP that are recognised by the rabbit polyclonal antibody and human serum, the antigenicities of three protein fragments, including the N-terminal domain (aa 1-173), the central-linker region (aa 174-300), and the C-terminal domain (aa 301-448), were evaluated by Western blot. The rabbit polyclonal antibody demonstrated greater immunoreactivity to the central-linker region and the C-terminal domain than to the N-terminal domain. Three different patterns for the immunoreactivities of the three structural regions of HCoV-OC43 NP were observed in human serum, suggesting variability in the immune responses that occur during HCoV-OC43 infection in humans. The central-linker region of the NP appeared to be the most highly immunoreactive region for all three patterns observed. The goal of this study was to offer insight into the design of diagnostic tools for HCoV infection.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0166-0934</s0></fA01><fA02 i1="01"><s0>JVMEDH</s0></fA02><fA03 i2="1"><s0>J. virol. methods</s0></fA03><fA05><s2>187</s2></fA05><fA06><s2>2</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infection</s1></fA08><fA11 i1="01" i2="1"><s1>LIANG (Fang-Ying)</s1></fA11><fA11 i1="02" i2="1"><s1>LIN (Leng-Chieh)</s1></fA11><fA11 i1="03" i2="1"><s1>YING (Tsung-Ho)</s1></fA11><fA11 i1="04" i2="1"><s1>YAO (Chen-Wen)</s1></fA11><fA11 i1="05" i2="1"><s1>TANG (Tswen-Kei)</s1></fA11><fA11 i1="06" i2="1"><s1>CHEN (Yi-Wen)</s1></fA11><fA11 i1="07" i2="1"><s1>HOU (Ming-Hon)</s1></fA11><fA14 i1="01"><s1>Biotechnology Center, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ></fA14><fA14 i1="02"><s1>Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>1 aut.</sZ></fA14><fA14 i1="03"><s1>Department of Emergency Medicine, Chang Gung Memorial Hospital</s1><s2>Puzih City, Chiayi</s2><s3>TWN</s3><sZ>2 aut.</sZ></fA14><fA14 i1="04"><s1>Departments of Nursing and Respiratory Care, Chang Gung Institute of Technology</s1><s2>Puzih City, Chiayi</s2><s3>TWN</s3><sZ>2 aut.</sZ></fA14><fA14 i1="05"><s1>Department of Obstetrics and Gynaecology, School of Medicine, College of Medicine, Chung Shan Medical University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>3 aut.</sZ></fA14><fA14 i1="06"><s1>Department of Pathology, Tri-Service General Hospital, National Defense University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>4 aut.</sZ><sZ>6 aut.</sZ></fA14><fA14 i1="07"><s1>Department of Medical Laboratory Science and Biotechnology, Yuanpei University</s1><s2>Hsinchu</s2><s3>TWN</s3><sZ>5 aut.</sZ></fA14><fA14 i1="08"><s1>College of Life Science, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>7 aut.</sZ></fA14><fA14 i1="09"><s1>Institute of Genomics and Bioinformatics, National Chung Hsing University</s1><s2>Taichung</s2><s3>TWN</s3><sZ>7 aut.</sZ></fA14><fA20><s1>413-420</s1></fA20><fA21><s1>2013</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18295</s2><s5>354000182506480350</s5></fA43><fA44><s0>0000</s0><s1>© 2013 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>3/4 p.</s0></fA45><fA47 i1="01" i2="1"><s0>13-0084742</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of virological methods</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>Previous studies have reported that a prokaryotic-expressed recombinant nucleocapsid protein (NP) is a suitable reagent for the epidemiological screening of coronavirus infection. In this study, soluble recombinant human coronavirus OC43 (HCoV-OC43) NP was produced to examine the antigenicity of the HCoV-OC43 NP of betacoronavirus. Using the purified recombinant NP as an antigen, a polyclonal antibody from rabbit serum with specificity for HCoV-OC43 NP was generated; this antibody reacts specifically with HCoV-OC43 NP and does not cross-react with other human CoV NPs (including those of SARS-CoV and HCoV-229E) by Western blot. Sera from 26 young adults, 17 middle-aged and elderly patients with respiratory infection, and 15 cord blood samples were also tested. Strong reactivity to the NPs of HCoV-OC43 was observed in 96%, 82%, and 93% of the serum samples from the young adults, respiratory patients, and cord blood samples, respectively. To identify the immunoreactivities of the three structural regions of the NP that are recognised by the rabbit polyclonal antibody and human serum, the antigenicities of three protein fragments, including the N-terminal domain (aa 1-173), the central-linker region (aa 174-300), and the C-terminal domain (aa 301-448), were evaluated by Western blot. The rabbit polyclonal antibody demonstrated greater immunoreactivity to the central-linker region and the C-terminal domain than to the N-terminal domain. Three different patterns for the immunoreactivities of the three structural regions of HCoV-OC43 NP were observed in human serum, suggesting variability in the immune responses that occur during HCoV-OC43 infection in humans. The central-linker region of the NP appeared to be the most highly immunoreactive region for all three patterns observed. The goal of this study was to offer insight into the design of diagnostic tools for HCoV infection.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C09</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus OC43 humain</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Human coronavirus OC43</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Human coronavirus OC43</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Homme</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Human</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Hombre</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Nucléocapside</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Nucleocapsid</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Nucleocápside</s0><s5>05</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Protéine</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Protein</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Proteína</s0><s5>06</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Méthode immunoblotting</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Immunoblotting assay</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Western blotting</s0><s5>07</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Diagnostic</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Diagnosis</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Diagnóstico</s0><s5>08</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Souche</s0><s5>09</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Strain</s0><s5>09</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Cepa</s0><s5>09</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Antigénicité</s0><s5>10</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Antigenicity</s0><s5>10</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Antigenicidad</s0><s5>10</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Anticorps</s0><s5>11</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Antibody</s0><s5>11</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Anticuerpo</s0><s5>11</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Virose</s0><s5>14</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Viral disease</s0><s5>14</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Virosis</s0><s5>14</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Infección</s0></fC07><fN21><s1>056</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000961 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 000961 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PascalFrancis
|étape= Curation
|type= RBID
|clé= Pascal:13-0084742
|texte= Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infection
}}
| This area was generated with Dilib version V0.6.33. |  |