SrasV1, PascalFrancis, Curation, bibRecord, 000960

Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease

Identifieur interne : 000960 ( PascalFrancis/Curation ); précédent : 000959; suivant : 000961

Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease

Auteurs : Shih-Wen Li [Taïwan] ; Tsuey-Ching Yang [Taïwan] ; LEI WAN [Taïwan] ; Ying-Ju Lin [Taïwan] ; Fuu-Jen Tsai [Taïwan] ; Chien-Chen Lai [Taïwan] ; Cheng-Wen Lin [Taïwan]

Source :

Proteomics : (Weinheim. Print) [ 1615-9853 ] ; 2012.

RBID : Pascal:13-0037501

Descripteurs français

Pascal (Inist)
- Coronavirus, Virus syndrome respiratoire aigu sévère, Protéomique, Papain, Ubiquitine, Multicatalytic endopeptidase complex, Vimentine, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Multicatalytic endopeptidase complex, Papain, Proteomics, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Ubiquitin, Vimentin.

Abstract

Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) papain-like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF-κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS-CoV PLpro in human promonocyte cells. PLpro significantly increased TGF-β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real-time PCR assays indicated PLpro upregulating TGF-β1-associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega-1. PLpro-activated ubiquitin proteasome pathway via upregulation of ubiquitin-conjugating enzyme E2-25k and proteasome subunit alpha type 5. Proteasome inhibitor MG-132 significantly reduced expression of TGF-β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatmentwith SB203580 and U0126 reduced PLpro-induced expression of TGF-β1, vimentin, and type I collagen. Results point to SARS-CoV PLpro triggering TGF-β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2-mediated signaling.

A01	`01`	`1`		`@0 1615-9853`
A03		`1`		`@0 Proteomics : (Weinh., Print)`
A05				`@2 12`
A06				`@2 21`
A08	`01`	`1`	`ENG`	`@1 Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease`
A11	`01`	`1`		`@1 LI (Shih-Wen)`
A11	`02`	`1`		`@1 YANG (Tsuey-Ching)`
A11	`03`	`1`		`@1 LEI WAN`
A11	`04`	`1`		`@1 LIN (Ying-Ju)`
A11	`05`	`1`		`@1 TSAI (Fuu-Jen)`
A11	`06`	`1`		`@1 LAI (Chien-Chen)`
A11	`07`	`1`		`@1 LIN (Cheng-Wen)`
A14	`01`			`@1 Department of Medical Laboratory Science and Biotechnology, China Medical University @2 Taichung @3 TWN @Z 1 aut. @Z 7 aut.`
A14	`02`			`@1 Institute of Molecular Biology, National Chung Hsing University @2 Taichung @3 TWN @Z 1 aut. @Z 6 aut.`
A14	`03`			`@1 Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University @2 Taipei @3 TWN @Z 2 aut.`
A14	`04`			`@1 Department of Medical Genetics and Medical Research, China Medical University Hospital @2 Taichung @3 TWN @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut.`
A14	`05`			`@1 Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital @2 Taichung @3 TWN @Z 7 aut.`
A14	`06`			`@1 Department of Biotechnology, Asia University @2 Taichung @3 TWN @Z 7 aut.`
A20				`@1 3193-3205`
A21				`@1 2012`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 27206 @5 354000505474710090`
A44				`@0 0000 @1 © 2013 INIST-CNRS. All rights reserved.`
A45				`@0 43 ref.`
A47	`01`	`1`		`@0 13-0037501`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Proteomics : (Weinheim. Print)`
A66	`01`			`@0 DEU`
C01	`01`		`ENG`	@0 Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) papain-like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF-κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS-CoV PLpro in human promonocyte cells. PLpro significantly increased TGF-β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real-time PCR assays indicated PLpro upregulating TGF-β1-associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega-1. PLpro-activated ubiquitin proteasome pathway via upregulation of ubiquitin-conjugating enzyme E2-25k and proteasome subunit alpha type 5. Proteasome inhibitor MG-132 significantly reduced expression of TGF-β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatmentwith SB203580 and U0126 reduced PLpro-induced expression of TGF-β1, vimentin, and type I collagen. Results point to SARS-CoV PLpro triggering TGF-β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2-mediated signaling.
C02	`01`	`X`		`@0 002A02D10`
C02	`02`	`X`		`@0 002B05C02C`
C03	`01`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Virus syndrome respiratoire aigu sévère @2 NW @5 02`
C03	`02`	`X`	`ENG`	`@0 Severe acute respiratory syndrome virus @2 NW @5 02`
C03	`02`	`X`	`SPA`	`@0 Severe acute respiratory syndrome virus @2 NW @5 02`
C03	`03`	`X`	`FRE`	`@0 Protéomique @5 05`
C03	`03`	`X`	`ENG`	`@0 Proteomics @5 05`
C03	`03`	`X`	`SPA`	`@0 Proteómica @5 05`
C03	`04`	`X`	`FRE`	`@0 Papain @2 FE @5 06`
C03	`04`	`X`	`ENG`	`@0 Papain @2 FE @5 06`
C03	`04`	`X`	`SPA`	`@0 Papain @2 FE @5 06`
C03	`05`	`X`	`FRE`	`@0 Ubiquitine @5 08`
C03	`05`	`X`	`ENG`	`@0 Ubiquitin @5 08`
C03	`05`	`X`	`SPA`	`@0 Ubiquitina @5 08`
C03	`06`	`X`	`FRE`	`@0 Multicatalytic endopeptidase complex @2 FE @5 09`
C03	`06`	`X`	`ENG`	`@0 Multicatalytic endopeptidase complex @2 FE @5 09`
C03	`06`	`X`	`SPA`	`@0 Multicatalytic endopeptidase complex @2 FE @5 09`
C03	`07`	`X`	`FRE`	`@0 Vimentine @5 10`
C03	`07`	`X`	`ENG`	`@0 Vimentin @5 10`
C03	`08`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
C03	`08`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`08`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
C07	`01`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`03`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`03`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`04`	`X`	`FRE`	`@0 Cysteine endopeptidases @2 FE`
C07	`04`	`X`	`ENG`	`@0 Cysteine endopeptidases @2 FE`
C07	`04`	`X`	`SPA`	`@0 Cysteine endopeptidases @2 FE`
C07	`05`	`X`	`FRE`	`@0 Peptidases @2 FE`
C07	`05`	`X`	`ENG`	`@0 Peptidases @2 FE`
C07	`05`	`X`	`SPA`	`@0 Peptidases @2 FE`
C07	`06`	`X`	`FRE`	`@0 Hydrolases @2 FE`
C07	`06`	`X`	`ENG`	`@0 Hydrolases @2 FE`
C07	`06`	`X`	`SPA`	`@0 Hydrolases @2 FE`
C07	`07`	`X`	`FRE`	`@0 Enzyme @2 FE`
C07	`07`	`X`	`ENG`	`@0 Enzyme @2 FE`
C07	`07`	`X`	`SPA`	`@0 Enzima @2 FE`
C07	`08`	`X`	`FRE`	`@0 Pathologie de l'appareil respiratoire @5 13`
C07	`08`	`X`	`ENG`	`@0 Respiratory disease @5 13`
C07	`08`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 13`
C07	`09`	`X`	`FRE`	`@0 Virose`
C07	`09`	`X`	`ENG`	`@0 Viral disease`
C07	`09`	`X`	`SPA`	`@0 Virosis`
C07	`10`	`X`	`FRE`	`@0 Infection`
C07	`10`	`X`	`ENG`	`@0 Infection`
C07	`10`	`X`	`SPA`	`@0 Infección`
C07	`11`	`X`	`FRE`	`@0 Pathologie des poumons @5 16`
C07	`11`	`X`	`ENG`	`@0 Lung disease @5 16`
C07	`11`	`X`	`SPA`	`@0 Pulmón patología @5 16`
N21				`@1 021`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000027

Links to Exploration step

Pascal:13-0037501

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease</title>
<author><name sortKey="Li, Shih Wen" sort="Li, Shih Wen" uniqKey="Li S" first="Shih-Wen" last="Li">Shih-Wen Li</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medical Laboratory Science and Biotechnology, China Medical University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Molecular Biology, National Chung Hsing University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Yang, Tsuey Ching" sort="Yang, Tsuey Ching" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lei Wan" sort="Lei Wan" uniqKey="Lei Wan" last="Lei Wan">LEI WAN</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lin, Ying Ju" sort="Lin, Ying Ju" uniqKey="Lin Y" first="Ying-Ju" last="Lin">Ying-Ju Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Tsai, Fuu Jen" sort="Tsai, Fuu Jen" uniqKey="Tsai F" first="Fuu-Jen" last="Tsai">Fuu-Jen Tsai</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lai, Chien Chen" sort="Lai, Chien Chen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Molecular Biology, National Chung Hsing University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lin, Cheng Wen" sort="Lin, Cheng Wen" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medical Laboratory Science and Biotechnology, China Medical University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Biotechnology, Asia University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">13-0037501</idno>
<date when="2012">2012</date>
<idno type="stanalyst">PASCAL 13-0037501 INIST</idno>
<idno type="RBID">Pascal:13-0037501</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000027</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000960</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease</title>
<author><name sortKey="Li, Shih Wen" sort="Li, Shih Wen" uniqKey="Li S" first="Shih-Wen" last="Li">Shih-Wen Li</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medical Laboratory Science and Biotechnology, China Medical University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Molecular Biology, National Chung Hsing University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Yang, Tsuey Ching" sort="Yang, Tsuey Ching" uniqKey="Yang T" first="Tsuey-Ching" last="Yang">Tsuey-Ching Yang</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lei Wan" sort="Lei Wan" uniqKey="Lei Wan" last="Lei Wan">LEI WAN</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lin, Ying Ju" sort="Lin, Ying Ju" uniqKey="Lin Y" first="Ying-Ju" last="Lin">Ying-Ju Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Tsai, Fuu Jen" sort="Tsai, Fuu Jen" uniqKey="Tsai F" first="Fuu-Jen" last="Tsai">Fuu-Jen Tsai</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lai, Chien Chen" sort="Lai, Chien Chen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Molecular Biology, National Chung Hsing University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
<author><name sortKey="Lin, Cheng Wen" sort="Lin, Cheng Wen" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Medical Laboratory Science and Biotechnology, China Medical University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Biotechnology, Asia University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Proteomics : (Weinheim. Print)</title>
<title level="j" type="abbreviated">Proteomics : (Weinh., Print)</title>
<idno type="ISSN">1615-9853</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Proteomics : (Weinheim. Print)</title>
<title level="j" type="abbreviated">Proteomics : (Weinh., Print)</title>
<idno type="ISSN">1615-9853</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Multicatalytic endopeptidase complex</term>
<term>Papain</term>
<term>Proteomics</term>
<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Ubiquitin</term>
<term>Vimentin</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Protéomique</term>
<term>Papain</term>
<term>Ubiquitine</term>
<term>Multicatalytic endopeptidase complex</term>
<term>Vimentine</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) papain-like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF-κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS-CoV PLpro in human promonocyte cells. PLpro significantly increased TGF-β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real-time PCR assays indicated PLpro upregulating TGF-β1-associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega-1. PLpro-activated ubiquitin proteasome pathway via upregulation of ubiquitin-conjugating enzyme E2-25k and proteasome subunit alpha type 5. Proteasome inhibitor MG-132 significantly reduced expression of TGF-β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatmentwith SB203580 and U0126 reduced PLpro-induced expression of TGF-β1, vimentin, and type I collagen. Results point to SARS-CoV PLpro triggering TGF-β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2-mediated signaling.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1615-9853</s0>
</fA01>
<fA03 i2="1"><s0>Proteomics : (Weinh., Print)</s0>
</fA03>
<fA05><s2>12</s2>
</fA05>
<fA06><s2>21</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LI (Shih-Wen)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>YANG (Tsuey-Ching)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>LEI WAN</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>LIN (Ying-Ju)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>TSAI (Fuu-Jen)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>LAI (Chien-Chen)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>LIN (Cheng-Wen)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Medical Laboratory Science and Biotechnology, China Medical University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Institute of Molecular Biology, National Chung Hsing University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Department of Biotechnology, Asia University</s1>
<s2>Taichung</s2>
<s3>TWN</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>3193-3205</s1>
</fA20>
<fA21><s1>2012</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>27206</s2>
<s5>354000505474710090</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2013 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>43 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>13-0037501</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Proteomics : (Weinheim. Print)</s0>
</fA64>
<fA66 i1="01"><s0>DEU</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) papain-like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF-κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS-CoV PLpro in human promonocyte cells. PLpro significantly increased TGF-β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real-time PCR assays indicated PLpro upregulating TGF-β1-associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega-1. PLpro-activated ubiquitin proteasome pathway via upregulation of ubiquitin-conjugating enzyme E2-25k and proteasome subunit alpha type 5. Proteasome inhibitor MG-132 significantly reduced expression of TGF-β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatmentwith SB203580 and U0126 reduced PLpro-induced expression of TGF-β1, vimentin, and type I collagen. Results point to SARS-CoV PLpro triggering TGF-β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2-mediated signaling.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A02D10</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002B05C02C</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Virus syndrome respiratoire aigu sévère</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Protéomique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Proteomics</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Proteómica</s0>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Papain</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Papain</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Papain</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Ubiquitine</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Ubiquitin</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Ubiquitina</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Vimentine</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Vimentin</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Cysteine endopeptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Cysteine endopeptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Cysteine endopeptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Pathologie de l'appareil respiratoire</s0>
<s5>13</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Pathologie des poumons</s0>
<s5>16</s5>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>021</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000960 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 000960 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PascalFrancis
   |étape=   Curation
   |type=    RBID
   |clé=     Pascal:13-0037501
   |texte=   Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease

Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri