Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease
Identifieur interne : 000027 ( PascalFrancis/Corpus ); précédent : 000026; suivant : 000028Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease
Auteurs : Shih-Wen Li ; Tsuey-Ching Yang ; LEI WAN ; Ying-Ju Lin ; Fuu-Jen Tsai ; Chien-Chen Lai ; Cheng-Wen LinSource :
- Proteomics : (Weinheim. Print) [ 1615-9853 ] ; 2012.
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Abstract
Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) papain-like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF-κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS-CoV PLpro in human promonocyte cells. PLpro significantly increased TGF-β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real-time PCR assays indicated PLpro upregulating TGF-β1-associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega-1. PLpro-activated ubiquitin proteasome pathway via upregulation of ubiquitin-conjugating enzyme E2-25k and proteasome subunit alpha type 5. Proteasome inhibitor MG-132 significantly reduced expression of TGF-β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatmentwith SB203580 and U0126 reduced PLpro-induced expression of TGF-β1, vimentin, and type I collagen. Results point to SARS-CoV PLpro triggering TGF-β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2-mediated signaling.
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Format Inist (serveur)
NO : | PASCAL 13-0037501 INIST |
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ET : | Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease |
AU : | LI (Shih-Wen); YANG (Tsuey-Ching); LEI WAN; LIN (Ying-Ju); TSAI (Fuu-Jen); LAI (Chien-Chen); LIN (Cheng-Wen) |
AF : | Department of Medical Laboratory Science and Biotechnology, China Medical University/Taichung/Taïwan (1 aut., 7 aut.); Institute of Molecular Biology, National Chung Hsing University/Taichung/Taïwan (1 aut., 6 aut.); Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University/Taipei/Taïwan (2 aut.); Department of Medical Genetics and Medical Research, China Medical University Hospital/Taichung/Taïwan (3 aut., 4 aut., 5 aut., 6 aut.); Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital/Taichung/Taïwan (7 aut.); Department of Biotechnology, Asia University/Taichung/Taïwan (7 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Proteomics : (Weinheim. Print); ISSN 1615-9853; Allemagne; Da. 2012; Vol. 12; No. 21; Pp. 3193-3205; Bibl. 43 ref. |
LA : | Anglais |
EA : | Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) papain-like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF-κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS-CoV PLpro in human promonocyte cells. PLpro significantly increased TGF-β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real-time PCR assays indicated PLpro upregulating TGF-β1-associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega-1. PLpro-activated ubiquitin proteasome pathway via upregulation of ubiquitin-conjugating enzyme E2-25k and proteasome subunit alpha type 5. Proteasome inhibitor MG-132 significantly reduced expression of TGF-β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatmentwith SB203580 and U0126 reduced PLpro-induced expression of TGF-β1, vimentin, and type I collagen. Results point to SARS-CoV PLpro triggering TGF-β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2-mediated signaling. |
CC : | 002A02D10; 002B05C02C |
FD : | Coronavirus; Virus syndrome respiratoire aigu sévère; Protéomique; Papain; Ubiquitine; Multicatalytic endopeptidase complex; Vimentine; Syndrome respiratoire aigu sévère |
FG : | Coronaviridae; Nidovirales; Virus; Cysteine endopeptidases; Peptidases; Hydrolases; Enzyme; Pathologie de l'appareil respiratoire; Virose; Infection; Pathologie des poumons |
ED : | Coronavirus; Severe acute respiratory syndrome virus; Proteomics; Papain; Ubiquitin; Multicatalytic endopeptidase complex; Vimentin; Severe acute respiratory syndrome |
EG : | Coronaviridae; Nidovirales; Virus; Cysteine endopeptidases; Peptidases; Hydrolases; Enzyme; Respiratory disease; Viral disease; Infection; Lung disease |
SD : | Coronavirus; Severe acute respiratory syndrome virus; Proteómica; Papain; Ubiquitina; Multicatalytic endopeptidase complex; Síndrome respiratorio agudo severo |
LO : | INIST-27206.354000505474710090 |
ID : | 13-0037501 |
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) papain-like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF-κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS-CoV PLpro in human promonocyte cells. PLpro significantly increased TGF-β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real-time PCR assays indicated PLpro upregulating TGF-β1-associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega-1. PLpro-activated ubiquitin proteasome pathway via upregulation of ubiquitin-conjugating enzyme E2-25k and proteasome subunit alpha type 5. Proteasome inhibitor MG-132 significantly reduced expression of TGF-β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatmentwith SB203580 and U0126 reduced PLpro-induced expression of TGF-β1, vimentin, and type I collagen. Results point to SARS-CoV PLpro triggering TGF-β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2-mediated signaling.</div>
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<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Ubiquitina</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Vimentine</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Vimentin</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Cysteine endopeptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Cysteine endopeptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Cysteine endopeptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Pathologie de l'appareil respiratoire</s0>
<s5>13</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Pathologie des poumons</s0>
<s5>16</s5>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>021</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 13-0037501 INIST</NO>
<ET>Correlation between TGF-β1 expression and proteomic profiling induced by severe acute respiratory syndrome coronavirus papain-like protease</ET>
<AU>LI (Shih-Wen); YANG (Tsuey-Ching); LEI WAN; LIN (Ying-Ju); TSAI (Fuu-Jen); LAI (Chien-Chen); LIN (Cheng-Wen)</AU>
<AF>Department of Medical Laboratory Science and Biotechnology, China Medical University/Taichung/Taïwan (1 aut., 7 aut.); Institute of Molecular Biology, National Chung Hsing University/Taichung/Taïwan (1 aut., 6 aut.); Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University/Taipei/Taïwan (2 aut.); Department of Medical Genetics and Medical Research, China Medical University Hospital/Taichung/Taïwan (3 aut., 4 aut., 5 aut., 6 aut.); Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital/Taichung/Taïwan (7 aut.); Department of Biotechnology, Asia University/Taichung/Taïwan (7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Proteomics : (Weinheim. Print); ISSN 1615-9853; Allemagne; Da. 2012; Vol. 12; No. 21; Pp. 3193-3205; Bibl. 43 ref.</SO>
<LA>Anglais</LA>
<EA>Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) papain-like protease (PLpro), a deubiquitinating enzyme, demonstrates inactivation of interferon (IFN) regulatory factor 3 and NF-κB, reduction of IFN induction, and suppression of type I IFN signaling pathway. This study investigates cytokine expression and proteomic change induced by SARS-CoV PLpro in human promonocyte cells. PLpro significantly increased TGF-β1 mRNA expression (greater than fourfold) and protein production (greater than threefold). Proteomic analysis, Western blot, and quantitative real-time PCR assays indicated PLpro upregulating TGF-β1-associated genes: HSP27, protein disulfide isomerase A3 precursor, glial fibrillary acidic protein, vimentin, retinal dehydrogenase 2, and glutathione transferase omega-1. PLpro-activated ubiquitin proteasome pathway via upregulation of ubiquitin-conjugating enzyme E2-25k and proteasome subunit alpha type 5. Proteasome inhibitor MG-132 significantly reduced expression of TGF-β1 and vimentin. PLpro upregulated HSP27, linking with activation of p38 MAPK and ERK1/2 signaling. Treatmentwith SB203580 and U0126 reduced PLpro-induced expression of TGF-β1, vimentin, and type I collagen. Results point to SARS-CoV PLpro triggering TGF-β1 production via ubiquitin proteasome, p38 MAPK, and ERK1/2-mediated signaling.</EA>
<CC>002A02D10; 002B05C02C</CC>
<FD>Coronavirus; Virus syndrome respiratoire aigu sévère; Protéomique; Papain; Ubiquitine; Multicatalytic endopeptidase complex; Vimentine; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Cysteine endopeptidases; Peptidases; Hydrolases; Enzyme; Pathologie de l'appareil respiratoire; Virose; Infection; Pathologie des poumons</FG>
<ED>Coronavirus; Severe acute respiratory syndrome virus; Proteomics; Papain; Ubiquitin; Multicatalytic endopeptidase complex; Vimentin; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Cysteine endopeptidases; Peptidases; Hydrolases; Enzyme; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Coronavirus; Severe acute respiratory syndrome virus; Proteómica; Papain; Ubiquitina; Multicatalytic endopeptidase complex; Síndrome respiratorio agudo severo</SD>
<LO>INIST-27206.354000505474710090</LO>
<ID>13-0037501</ID>
</server>
</inist>
</record>
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