A universal microarray for detection of SARS coronavirus
Identifieur interne : 000195 ( PascalFrancis/Curation ); précédent : 000194; suivant : 000196A universal microarray for detection of SARS coronavirus
Auteurs : Wei-Hong Long [République populaire de Chine] ; Hua-Sheng Xiao [République populaire de Chine] ; Xiao-Mei Gu [République populaire de Chine] ; Qing-Hua Zhang [République populaire de Chine] ; Hong-Jun Yang [République populaire de Chine] ; Guo-Ping Zhao [République populaire de Chine] ; Jian-Hua Liu [République populaire de Chine]Source :
- Journal of virological methods [ 0166-0934 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.
pA |
|
---|
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000795
Links to Exploration step
Pascal:04-0535876Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">A universal microarray for detection of SARS coronavirus</title>
<author><name sortKey="Long, Wei Hong" sort="Long, Wei Hong" uniqKey="Long W" first="Wei-Hong" last="Long">Wei-Hong Long</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road</s1>
<s2>Shanghai 200030</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Xiao, Hua Sheng" sort="Xiao, Hua Sheng" uniqKey="Xiao H" first="Hua-Sheng" last="Xiao">Hua-Sheng Xiao</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Gu, Xiao Mei" sort="Gu, Xiao Mei" uniqKey="Gu X" first="Xiao-Mei" last="Gu">Xiao-Mei Gu</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Zhang, Qing Hua" sort="Zhang, Qing Hua" uniqKey="Zhang Q" first="Qing-Hua" last="Zhang">Qing-Hua Zhang</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Yang, Hong Jun" sort="Yang, Hong Jun" uniqKey="Yang H" first="Hong-Jun" last="Yang">Hong-Jun Yang</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Zhao, Guo Ping" sort="Zhao, Guo Ping" uniqKey="Zhao G" first="Guo-Ping" last="Zhao">Guo-Ping Zhao</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Liu, Jian Hua" sort="Liu, Jian Hua" uniqKey="Liu J" first="Jian-Hua" last="Liu">Jian-Hua Liu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road</s1>
<s2>Shanghai 200030</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">04-0535876</idno>
<date when="2004">2004</date>
<idno type="stanalyst">PASCAL 04-0535876 INIST</idno>
<idno type="RBID">Pascal:04-0535876</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000795</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000195</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">A universal microarray for detection of SARS coronavirus</title>
<author><name sortKey="Long, Wei Hong" sort="Long, Wei Hong" uniqKey="Long W" first="Wei-Hong" last="Long">Wei-Hong Long</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road</s1>
<s2>Shanghai 200030</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Xiao, Hua Sheng" sort="Xiao, Hua Sheng" uniqKey="Xiao H" first="Hua-Sheng" last="Xiao">Hua-Sheng Xiao</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Gu, Xiao Mei" sort="Gu, Xiao Mei" uniqKey="Gu X" first="Xiao-Mei" last="Gu">Xiao-Mei Gu</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Zhang, Qing Hua" sort="Zhang, Qing Hua" uniqKey="Zhang Q" first="Qing-Hua" last="Zhang">Qing-Hua Zhang</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Yang, Hong Jun" sort="Yang, Hong Jun" uniqKey="Yang H" first="Hong-Jun" last="Yang">Hong-Jun Yang</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Zhao, Guo Ping" sort="Zhao, Guo Ping" uniqKey="Zhao G" first="Guo-Ping" last="Zhao">Guo-Ping Zhao</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Liu, Jian Hua" sort="Liu, Jian Hua" uniqKey="Liu J" first="Jian-Hua" last="Liu">Jian-Hua Liu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road</s1>
<s2>Shanghai 200030</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of virological methods</title>
<title level="j" type="abbreviated">J. virol. methods</title>
<idno type="ISSN">0166-0934</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of virological methods</title>
<title level="j" type="abbreviated">J. virol. methods</title>
<idno type="ISSN">0166-0934</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Detection</term>
<term>Method</term>
<term>Microbiology</term>
<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Virology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Détection</term>
<term>Microbiologie</term>
<term>Méthode</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0166-0934</s0>
</fA01>
<fA02 i1="01"><s0>JVMEDH</s0>
</fA02>
<fA03 i2="1"><s0>J. virol. methods</s0>
</fA03>
<fA05><s2>121</s2>
</fA05>
<fA06><s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>A universal microarray for detection of SARS coronavirus</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LONG (Wei-Hong)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>XIAO (Hua-Sheng)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>GU (Xiao-Mei)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>ZHANG (Qing-Hua)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>YANG (Hong-Jun)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>ZHAO (Guo-Ping)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>LIU (Jian-Hua)</s1>
</fA11>
<fA14 i1="01"><s1>School of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road</s1>
<s2>Shanghai 200030</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Shanghai BioChip Co. Ltd., 151 Li-Bing Road</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA20><s1>57-63</s1>
</fA20>
<fA21><s1>2004</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>18295</s2>
<s5>354000120328020080</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2004 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>15 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>04-0535876</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of virological methods</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C09</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Détection</s0>
<s5>05</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Detection</s0>
<s5>05</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Detección</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>06</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>06</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Méthode</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Method</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Método</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Virologie</s0>
<s5>08</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Virology</s0>
<s5>08</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Virología</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Virus syndrome respiratoire aigu sévère</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Severe acute respiratory syndrome virus</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Viral disease</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Virosis</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fN21><s1>306</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000195 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 000195 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PascalFrancis |étape= Curation |type= RBID |clé= Pascal:04-0535876 |texte= A universal microarray for detection of SARS coronavirus }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |