SrasV1, PascalFrancis, Corpus, bibRecord, 000795

A universal microarray for detection of SARS coronavirus

Identifieur interne : 000795 ( PascalFrancis/Corpus ); précédent : 000794; suivant : 000796

A universal microarray for detection of SARS coronavirus

Auteurs : Wei-Hong Long ; Hua-Sheng Xiao ; Xiao-Mei Gu ; Qing-Hua Zhang ; Hong-Jun Yang ; Guo-Ping Zhao ; Jian-Hua Liu

Source :

Journal of virological methods [ 0166-0934 ] ; 2004.

RBID : Pascal:04-0535876

Descripteurs français

Pascal (Inist)
- Détection, Microbiologie, Méthode, Virologie, Syndrome respiratoire aigu sévère, Virus syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Detection, Method, Microbiology, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Virology.

Abstract

Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0166-0934`
A02	`01`			`@0 JVMEDH`
A03		`1`		`@0 J. virol. methods`
A05				`@2 121`
A06				`@2 1`
A08	`01`	`1`	`ENG`	`@1 A universal microarray for detection of SARS coronavirus`
A11	`01`	`1`		`@1 LONG (Wei-Hong)`
A11	`02`	`1`		`@1 XIAO (Hua-Sheng)`
A11	`03`	`1`		`@1 GU (Xiao-Mei)`
A11	`04`	`1`		`@1 ZHANG (Qing-Hua)`
A11	`05`	`1`		`@1 YANG (Hong-Jun)`
A11	`06`	`1`		`@1 ZHAO (Guo-Ping)`
A11	`07`	`1`		`@1 LIU (Jian-Hua)`
A14	`01`			`@1 School of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road @2 Shanghai 200030 @3 CHN @Z 1 aut. @Z 7 aut.`
A14	`02`			`@1 Shanghai BioChip Co. Ltd., 151 Li-Bing Road @2 Shanghai 201203 @3 CHN @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut.`
A20				`@1 57-63`
A21				`@1 2004`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 18295 @5 354000120328020080`
A44				`@0 0000 @1 © 2004 INIST-CNRS. All rights reserved.`
A45				`@0 15 ref.`
A47	`01`	`1`		`@0 04-0535876`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of virological methods`
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C01	`01`		`ENG`	@0 Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.
C02	`01`	`X`		`@0 002A05C09`
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C03	`01`	`X`	`ENG`	`@0 Detection @5 05`
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C03	`02`	`X`	`FRE`	`@0 Microbiologie @5 06`
C03	`02`	`X`	`ENG`	`@0 Microbiology @5 06`
C03	`02`	`X`	`SPA`	`@0 Microbiología @5 06`
C03	`03`	`X`	`FRE`	`@0 Méthode @5 07`
C03	`03`	`X`	`ENG`	`@0 Method @5 07`
C03	`03`	`X`	`SPA`	`@0 Método @5 07`
C03	`04`	`X`	`FRE`	`@0 Virologie @5 08`
C03	`04`	`X`	`ENG`	`@0 Virology @5 08`
C03	`04`	`X`	`SPA`	`@0 Virología @5 08`
C03	`05`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
C03	`05`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`05`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
C03	`06`	`X`	`FRE`	`@0 Virus syndrome respiratoire aigu sévère @4 CD @5 96`
C03	`06`	`X`	`ENG`	`@0 Severe acute respiratory syndrome virus @4 CD @5 96`
C03	`06`	`X`	`SPA`	`@0 Severe acute respiratory syndrome virus @4 CD @5 96`
C07	`01`	`X`	`FRE`	`@0 Virose @2 NM`
C07	`01`	`X`	`ENG`	`@0 Viral disease @2 NM`
C07	`01`	`X`	`SPA`	`@0 Virosis @2 NM`
C07	`02`	`X`	`FRE`	`@0 Infection @2 NM`
C07	`02`	`X`	`ENG`	`@0 Infection @2 NM`
C07	`02`	`X`	`SPA`	`@0 Infección @2 NM`
N21				`@1 306`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 04-0535876 INIST
ET :	A universal microarray for detection of SARS coronavirus
AU :	LONG (Wei-Hong); XIAO (Hua-Sheng); GU (Xiao-Mei); ZHANG (Qing-Hua); YANG (Hong-Jun); ZHAO (Guo-Ping); LIU (Jian-Hua)
AF :	School of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road/Shanghai 200030/Chine (1 aut., 7 aut.); Shanghai BioChip Co. Ltd., 151 Li-Bing Road/Shanghai 201203/Chine (2 aut., 3 aut., 4 aut., 5 aut., 6 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of virological methods; ISSN 0166-0934; Coden JVMEDH; Pays-Bas; Da. 2004; Vol. 121; No. 1; Pp. 57-63; Bibl. 15 ref.
LA :	Anglais
EA :	Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.
CC :	002A05C09
FD :	Détection; Microbiologie; Méthode; Virologie; Syndrome respiratoire aigu sévère; Virus syndrome respiratoire aigu sévère
FG :	Virose; Infection
ED :	Detection; Microbiology; Method; Virology; Severe acute respiratory syndrome; Severe acute respiratory syndrome virus
EG :	Viral disease; Infection
SD :	Detección; Microbiología; Método; Virología; Síndrome respiratorio agudo severo; Severe acute respiratory syndrome virus
LO :	INIST-18295.354000120328020080
ID :	04-0535876

Links to Exploration step

Pascal:04-0535876

Le document en format XML

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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.</div>
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<fC01 i1="01" l="ENG"><s0>Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.</s0>
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<server><NO>PASCAL 04-0535876 INIST</NO>
<ET>A universal microarray for detection of SARS coronavirus</ET>
<AU>LONG (Wei-Hong); XIAO (Hua-Sheng); GU (Xiao-Mei); ZHANG (Qing-Hua); YANG (Hong-Jun); ZHAO (Guo-Ping); LIU (Jian-Hua)</AU>
<AF>School of Life Science and Technology, Shanghai JiaoTong University, 1954 Hua-Shan Road/Shanghai 200030/Chine (1 aut., 7 aut.); Shanghai BioChip Co. Ltd., 151 Li-Bing Road/Shanghai 201203/Chine (2 aut., 3 aut., 4 aut., 5 aut., 6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virological methods; ISSN 0166-0934; Coden JVMEDH; Pays-Bas; Da. 2004; Vol. 121; No. 1; Pp. 57-63; Bibl. 15 ref.</SO>
<LA>Anglais</LA>
<EA>Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (SARS-CoV). There are many point mutations among SARS-CoV genome sequences. Previous studies suggested that the mutations are correlated closely with the SARS epidemic. It was found that the bases of six nucleotide positions (nt9404, nt9479, nt 19838, nt21721, nt22222 and nt27827) with high-mutation rate have an important relationship with the SARS epidemic. For viral detection as well as genotyping, a universal microarray system was developed that combines RT-PCR and ligase detection reaction (LDR). The Zip Codes attached covalently to a slide remain constant and their complementary Zip Codes (cZip Codes) can be used for tagging target sequence, making the microarrays universal. The discriminating oligonucleotides contain on the 5' end "cZip Codes" that are used to direct LDR product to specific Zip Codes attached covalently to a slide. Since Zip Codes have no homology to either the target sequence or to other sequences in the genomes of both human host and SARS-CoV, there was no false signal due to mismatch hybridizations. 20 samples assayed with the universal microarray were confirmed by DNA sequencing, demonstrating that this microarray system is a promising diagnostic tool for detection and genotyping of the SARS-CoV.</EA>
<CC>002A05C09</CC>
<FD>Détection; Microbiologie; Méthode; Virologie; Syndrome respiratoire aigu sévère; Virus syndrome respiratoire aigu sévère</FD>
<FG>Virose; Infection</FG>
<ED>Detection; Microbiology; Method; Virology; Severe acute respiratory syndrome; Severe acute respiratory syndrome virus</ED>
<EG>Viral disease; Infection</EG>
<SD>Detección; Microbiología; Método; Virología; Síndrome respiratorio agudo severo; Severe acute respiratory syndrome virus</SD>
<LO>INIST-18295.354000120328020080</LO>
<ID>04-0535876</ID>
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A universal microarray for detection of SARS coronavirus

A universal microarray for detection of SARS coronavirus

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