SrasV1, PascalFrancis, Corpus, bibRecord, 000573

Detection of severe acute respiratory syndrome coronavirus in the brain : Potential role of the chemokine mig in pathogenesis

Identifieur interne : 000573 ( PascalFrancis/Corpus ); précédent : 000572; suivant : 000574

Detection of severe acute respiratory syndrome coronavirus in the brain : Potential role of the chemokine mig in pathogenesis

Auteurs : JUN XU ; SHUQING ZHONG ; JINGHUA LIU ; LI LI ; YONG LI ; XINWEI WU ; ZHIJIE LI ; PENG DENG ; JINGQIANG ZHANG ; NANSHAN ZHONG ; YANQING DING ; YONG JIANG

Source :

Clinical infectious diseases [ 1058-4838 ] ; 2005.

RBID : Pascal:06-0000179

Descripteurs français

Pascal (Inist)
- Syndrome respiratoire aigu sévère, Encéphale, Cerveau, Chimiokine, Coronavirus, Pathogénie.

English descriptors

KwdEn :
- Brain, Chemokine, Coronavirus, Encephalon, Pathogenesis, Severe acute respiratory syndrome.

Abstract

Background. Previous studies have shown that common human coronavirus might be neurotropic, although it was first isolated as a pathogen of the respiratory tract. We noticed that a few patients with severe acute respiratory syndrome (SARS) experienced central nervous symptoms during the course of illness. In the present study, we isolated a SARS coronavirus strain from a brain tissue specimen obtained from a patient with SARS with significant central nervous symptoms. Methods. Using transmission electronic microscopy and nested reverse transcription-polymerase chain reaction, the causative pathogen was identified in cultures of a brain tissue specimen obtained from the patient with SARS. Histopathologic examination of the brain tissue was performed using the methods of immunohistochemistry analysis and double immunofluorescence staining. Fifteen cytokines and chemokines were detected in the blood of the patient with SARS by means of a bead-based multiassay system. Results. A fragment specific for SARS human coronavirus was amplified from cultures of the brain suspension, and transmission electronic microscopy revealed the presence of an enveloped virus morphologically compatible with a coronavirus isolated in the cultures. Pathologic examination of the brain tissue revealed necrosis of neuron cells and broad hyperplasia of gliocytes. Immunostaining demonstrated that monokine induced by interferon-γ (Mig) was expressed in gliocytes with the infiltration of CD68⁺ monocytes/macrophages and CD3⁺ T lymphocytes in the brain mesenchyme. Cytokine/chemokine assay revealed that levels of interferon-γ-inducible protein 10 and Mig in the blood were highly elevated, although the levels of other cytokines and chemokines were close to normal. Conclusions. This study provides direct evidence that SARS human coronavirus is capable of infecting the central nervous system, and that Mig might be involved in the brain immunopathology of SARS.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 1058-4838`
A02	`01`			`@0 CIDIEL`
A03		`1`		`@0 Clin. infect. dis.`
A05				`@2 41`
A06				`@2 8`
A08	`01`	`1`	`ENG`	`@1 Detection of severe acute respiratory syndrome coronavirus in the brain : Potential role of the chemokine mig in pathogenesis`
A11	`01`	`1`		`@1 JUN XU`
A11	`02`	`1`		`@1 SHUQING ZHONG`
A11	`03`	`1`		`@1 JINGHUA LIU`
A11	`04`	`1`		`@1 LI LI`
A11	`05`	`1`		`@1 YONG LI`
A11	`06`	`1`		`@1 XINWEI WU`
A11	`07`	`1`		`@1 ZHIJIE LI`
A11	`08`	`1`		`@1 PENG DENG`
A11	`09`	`1`		`@1 JINGQIANG ZHANG`
A11	`10`	`1`		`@1 NANSHAN ZHONG`
A11	`11`	`1`		`@1 YANQING DING`
A11	`12`	`1`		`@1 YONG JIANG`
A14	`01`			`@1 Guangzhou Institute of Respiratory Diseases, Southern Medical University @3 CHN @Z 1 aut. @Z 2 aut. @Z 4 aut. @Z 5 aut. @Z 10 aut.`
A14	`02`			`@1 Key Laboratory of Functional Proteomics of Guangdong Province, Southern Medical University @3 CHN @Z 3 aut. @Z 7 aut. @Z 8 aut. @Z 11 aut. @Z 12 aut.`
A14	`03`			`@1 Guangzhou Center for Diseases Control and Prevention, Sun Yatsen University @2 Guangzhou @3 CHN @Z 6 aut.`
A14	`04`			`@1 Electronic Microscope Center, Sun Yatsen University @2 Guangzhou @3 CHN @Z 9 aut.`
A20				`@1 1089-1096`
A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 18407 @5 354000131931080020`
A44				`@0 0000 @1 © 2006 INIST-CNRS. All rights reserved.`
A45				`@0 30 ref.`
A47	`01`	`1`		`@0 06-0000179`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Clinical infectious diseases`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Background. Previous studies have shown that common human coronavirus might be neurotropic, although it was first isolated as a pathogen of the respiratory tract. We noticed that a few patients with severe acute respiratory syndrome (SARS) experienced central nervous symptoms during the course of illness. In the present study, we isolated a SARS coronavirus strain from a brain tissue specimen obtained from a patient with SARS with significant central nervous symptoms. Methods. Using transmission electronic microscopy and nested reverse transcription-polymerase chain reaction, the causative pathogen was identified in cultures of a brain tissue specimen obtained from the patient with SARS. Histopathologic examination of the brain tissue was performed using the methods of immunohistochemistry analysis and double immunofluorescence staining. Fifteen cytokines and chemokines were detected in the blood of the patient with SARS by means of a bead-based multiassay system. Results. A fragment specific for SARS human coronavirus was amplified from cultures of the brain suspension, and transmission electronic microscopy revealed the presence of an enveloped virus morphologically compatible with a coronavirus isolated in the cultures. Pathologic examination of the brain tissue revealed necrosis of neuron cells and broad hyperplasia of gliocytes. Immunostaining demonstrated that monokine induced by interferon-γ (Mig) was expressed in gliocytes with the infiltration of CD68⁺ monocytes/macrophages and CD3⁺ T lymphocytes in the brain mesenchyme. Cytokine/chemokine assay revealed that levels of interferon-γ-inducible protein 10 and Mig in the blood were highly elevated, although the levels of other cytokines and chemokines were close to normal. Conclusions. This study provides direct evidence that SARS human coronavirus is capable of infecting the central nervous system, and that Mig might be involved in the brain immunopathology of SARS.
C02	`01`	`X`		`@0 002B05C02C`
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C03	`01`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 01`
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C03	`02`	`X`	`FRE`	`@0 Encéphale @5 07`
C03	`02`	`X`	`ENG`	`@0 Encephalon @5 07`
C03	`02`	`X`	`SPA`	`@0 Encéfalo @5 07`
C03	`03`	`X`	`FRE`	`@0 Cerveau @5 08`
C03	`03`	`X`	`ENG`	`@0 Brain @5 08`
C03	`03`	`X`	`SPA`	`@0 Cerebro @5 08`
C03	`04`	`X`	`FRE`	`@0 Chimiokine @5 09`
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C03	`04`	`X`	`SPA`	`@0 Quimioquina @5 09`
C03	`05`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 10`
C03	`05`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 10`
C03	`05`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 10`
C03	`06`	`X`	`FRE`	`@0 Pathogénie @5 13`
C03	`06`	`X`	`ENG`	`@0 Pathogenesis @5 13`
C03	`06`	`X`	`SPA`	`@0 Patogenia @5 13`
C07	`01`	`X`	`FRE`	`@0 Virose`
C07	`01`	`X`	`ENG`	`@0 Viral disease`
C07	`01`	`X`	`SPA`	`@0 Virosis`
C07	`02`	`X`	`FRE`	`@0 Infection`
C07	`02`	`X`	`ENG`	`@0 Infection`
C07	`02`	`X`	`SPA`	`@0 Infección`
C07	`03`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`03`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`03`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`04`	`X`	`FRE`	`@0 Nidovirales @2 NW`
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C07	`07`	`X`	`FRE`	`@0 Poumon pathologie @5 38`
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C07	`07`	`X`	`SPA`	`@0 Pulmón patología @5 38`
N21				`@1 002`
N44	`01`			`@1 OTO`
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Format Inist (serveur)

NO :	PASCAL 06-0000179 INIST
ET :	Detection of severe acute respiratory syndrome coronavirus in the brain : Potential role of the chemokine mig in pathogenesis
AU :	JUN XU; SHUQING ZHONG; JINGHUA LIU; LI LI; YONG LI; XINWEI WU; ZHIJIE LI; PENG DENG; JINGQIANG ZHANG; NANSHAN ZHONG; YANQING DING; YONG JIANG
AF :	Guangzhou Institute of Respiratory Diseases, Southern Medical University/Chine (1 aut., 2 aut., 4 aut., 5 aut., 10 aut.); Key Laboratory of Functional Proteomics of Guangdong Province, Southern Medical University/Chine (3 aut., 7 aut., 8 aut., 11 aut., 12 aut.); Guangzhou Center for Diseases Control and Prevention, Sun Yatsen University/Guangzhou/Chine (6 aut.); Electronic Microscope Center, Sun Yatsen University/Guangzhou/Chine (9 aut.)
DT :	Publication en série; Niveau analytique
SO :	Clinical infectious diseases; ISSN 1058-4838; Coden CIDIEL; Etats-Unis; Da. 2005; Vol. 41; No. 8; Pp. 1089-1096; Bibl. 30 ref.
LA :	Anglais
EA :	Background. Previous studies have shown that common human coronavirus might be neurotropic, although it was first isolated as a pathogen of the respiratory tract. We noticed that a few patients with severe acute respiratory syndrome (SARS) experienced central nervous symptoms during the course of illness. In the present study, we isolated a SARS coronavirus strain from a brain tissue specimen obtained from a patient with SARS with significant central nervous symptoms. Methods. Using transmission electronic microscopy and nested reverse transcription-polymerase chain reaction, the causative pathogen was identified in cultures of a brain tissue specimen obtained from the patient with SARS. Histopathologic examination of the brain tissue was performed using the methods of immunohistochemistry analysis and double immunofluorescence staining. Fifteen cytokines and chemokines were detected in the blood of the patient with SARS by means of a bead-based multiassay system. Results. A fragment specific for SARS human coronavirus was amplified from cultures of the brain suspension, and transmission electronic microscopy revealed the presence of an enveloped virus morphologically compatible with a coronavirus isolated in the cultures. Pathologic examination of the brain tissue revealed necrosis of neuron cells and broad hyperplasia of gliocytes. Immunostaining demonstrated that monokine induced by interferon-γ (Mig) was expressed in gliocytes with the infiltration of CD68⁺ monocytes/macrophages and CD3⁺ T lymphocytes in the brain mesenchyme. Cytokine/chemokine assay revealed that levels of interferon-γ-inducible protein 10 and Mig in the blood were highly elevated, although the levels of other cytokines and chemokines were close to normal. Conclusions. This study provides direct evidence that SARS human coronavirus is capable of infecting the central nervous system, and that Mig might be involved in the brain immunopathology of SARS.
CC :	002B05C02C
FD :	Syndrome respiratoire aigu sévère; Encéphale; Cerveau; Chimiokine; Coronavirus; Pathogénie
FG :	Virose; Infection; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Poumon pathologie
ED :	Severe acute respiratory syndrome; Encephalon; Brain; Chemokine; Coronavirus; Pathogenesis
EG :	Viral disease; Infection; Coronaviridae; Nidovirales; Virus; Respiratory disease; Lung disease
SD :	Síndrome respiratorio agudo severo; Encéfalo; Cerebro; Quimioquina; Coronavirus; Patogenia
LO :	INIST-18407.354000131931080020
ID :	06-0000179

Links to Exploration step

Pascal:06-0000179

Le document en format XML

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<author><name sortKey="Jun Xu" sort="Jun Xu" uniqKey="Jun Xu" last="Jun Xu">JUN XU</name>
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<author><name sortKey="Li Li" sort="Li Li" uniqKey="Li Li" last="Li Li">LI LI</name>
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<author><name sortKey="Zhijie Li" sort="Zhijie Li" uniqKey="Zhijie Li" last="Zhijie Li">ZHIJIE LI</name>
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<author><name sortKey="Peng Deng" sort="Peng Deng" uniqKey="Peng Deng" last="Peng Deng">PENG DENG</name>
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<author><name sortKey="Jingqiang Zhang" sort="Jingqiang Zhang" uniqKey="Jingqiang Zhang" last="Jingqiang Zhang">JINGQIANG ZHANG</name>
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<author><name sortKey="Nanshan Zhong" sort="Nanshan Zhong" uniqKey="Nanshan Zhong" last="Nanshan Zhong">NANSHAN ZHONG</name>
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<author><name sortKey="Yanqing Ding" sort="Yanqing Ding" uniqKey="Yanqing Ding" last="Yanqing Ding">YANQING DING</name>
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<author><name sortKey="Yong Jiang" sort="Yong Jiang" uniqKey="Yong Jiang" last="Yong Jiang">YONG JIANG</name>
<affiliation><inist:fA14 i1="02"><s1>Key Laboratory of Functional Proteomics of Guangdong Province, Southern Medical University</s1>
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<series><title level="j" type="main">Clinical infectious diseases</title>
<title level="j" type="abbreviated">Clin. infect. dis.</title>
<idno type="ISSN">1058-4838</idno>
<imprint><date when="2005">2005</date>
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<seriesStmt><title level="j" type="main">Clinical infectious diseases</title>
<title level="j" type="abbreviated">Clin. infect. dis.</title>
<idno type="ISSN">1058-4838</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Brain</term>
<term>Chemokine</term>
<term>Coronavirus</term>
<term>Encephalon</term>
<term>Pathogenesis</term>
<term>Severe acute respiratory syndrome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
<term>Encéphale</term>
<term>Cerveau</term>
<term>Chimiokine</term>
<term>Coronavirus</term>
<term>Pathogénie</term>
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<front><div type="abstract" xml:lang="en">Background. Previous studies have shown that common human coronavirus might be neurotropic, although it was first isolated as a pathogen of the respiratory tract. We noticed that a few patients with severe acute respiratory syndrome (SARS) experienced central nervous symptoms during the course of illness. In the present study, we isolated a SARS coronavirus strain from a brain tissue specimen obtained from a patient with SARS with significant central nervous symptoms. Methods. Using transmission electronic microscopy and nested reverse transcription-polymerase chain reaction, the causative pathogen was identified in cultures of a brain tissue specimen obtained from the patient with SARS. Histopathologic examination of the brain tissue was performed using the methods of immunohistochemistry analysis and double immunofluorescence staining. Fifteen cytokines and chemokines were detected in the blood of the patient with SARS by means of a bead-based multiassay system. Results. A fragment specific for SARS human coronavirus was amplified from cultures of the brain suspension, and transmission electronic microscopy revealed the presence of an enveloped virus morphologically compatible with a coronavirus isolated in the cultures. Pathologic examination of the brain tissue revealed necrosis of neuron cells and broad hyperplasia of gliocytes. Immunostaining demonstrated that monokine induced by interferon-γ (Mig) was expressed in gliocytes with the infiltration of CD68<sup>+</sup>
 monocytes/macrophages and CD3<sup>+</sup>
 T lymphocytes in the brain mesenchyme. Cytokine/chemokine assay revealed that levels of interferon-γ-inducible protein 10 and Mig in the blood were highly elevated, although the levels of other cytokines and chemokines were close to normal. Conclusions. This study provides direct evidence that SARS human coronavirus is capable of infecting the central nervous system, and that Mig might be involved in the brain immunopathology of SARS.</div>
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<fA11 i1="01" i2="1"><s1>JUN XU</s1>
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<fA11 i1="02" i2="1"><s1>SHUQING ZHONG</s1>
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<fA11 i1="03" i2="1"><s1>JINGHUA LIU</s1>
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<fA11 i1="04" i2="1"><s1>LI LI</s1>
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<fA11 i1="05" i2="1"><s1>YONG LI</s1>
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<fA11 i1="07" i2="1"><s1>ZHIJIE LI</s1>
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<fA11 i1="08" i2="1"><s1>PENG DENG</s1>
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<fA11 i1="09" i2="1"><s1>JINGQIANG ZHANG</s1>
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<fA11 i1="10" i2="1"><s1>NANSHAN ZHONG</s1>
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<fA11 i1="11" i2="1"><s1>YANQING DING</s1>
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<fA11 i1="12" i2="1"><s1>YONG JIANG</s1>
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<fA14 i1="01"><s1>Guangzhou Institute of Respiratory Diseases, Southern Medical University</s1>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
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<fA14 i1="02"><s1>Key Laboratory of Functional Proteomics of Guangdong Province, Southern Medical University</s1>
<s3>CHN</s3>
<sZ>3 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
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<fA14 i1="03"><s1>Guangzhou Center for Diseases Control and Prevention, Sun Yatsen University</s1>
<s2>Guangzhou</s2>
<s3>CHN</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Electronic Microscope Center, Sun Yatsen University</s1>
<s2>Guangzhou</s2>
<s3>CHN</s3>
<sZ>9 aut.</sZ>
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 monocytes/macrophages and CD3<sup>+</sup>
 T lymphocytes in the brain mesenchyme. Cytokine/chemokine assay revealed that levels of interferon-γ-inducible protein 10 and Mig in the blood were highly elevated, although the levels of other cytokines and chemokines were close to normal. Conclusions. This study provides direct evidence that SARS human coronavirus is capable of infecting the central nervous system, and that Mig might be involved in the brain immunopathology of SARS.</s0>
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<server><NO>PASCAL 06-0000179 INIST</NO>
<ET>Detection of severe acute respiratory syndrome coronavirus in the brain : Potential role of the chemokine mig in pathogenesis</ET>
<AU>JUN XU; SHUQING ZHONG; JINGHUA LIU; LI LI; YONG LI; XINWEI WU; ZHIJIE LI; PENG DENG; JINGQIANG ZHANG; NANSHAN ZHONG; YANQING DING; YONG JIANG</AU>
<AF>Guangzhou Institute of Respiratory Diseases, Southern Medical University/Chine (1 aut., 2 aut., 4 aut., 5 aut., 10 aut.); Key Laboratory of Functional Proteomics of Guangdong Province, Southern Medical University/Chine (3 aut., 7 aut., 8 aut., 11 aut., 12 aut.); Guangzhou Center for Diseases Control and Prevention, Sun Yatsen University/Guangzhou/Chine (6 aut.); Electronic Microscope Center, Sun Yatsen University/Guangzhou/Chine (9 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Clinical infectious diseases; ISSN 1058-4838; Coden CIDIEL; Etats-Unis; Da. 2005; Vol. 41; No. 8; Pp. 1089-1096; Bibl. 30 ref.</SO>
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<EA>Background. Previous studies have shown that common human coronavirus might be neurotropic, although it was first isolated as a pathogen of the respiratory tract. We noticed that a few patients with severe acute respiratory syndrome (SARS) experienced central nervous symptoms during the course of illness. In the present study, we isolated a SARS coronavirus strain from a brain tissue specimen obtained from a patient with SARS with significant central nervous symptoms. Methods. Using transmission electronic microscopy and nested reverse transcription-polymerase chain reaction, the causative pathogen was identified in cultures of a brain tissue specimen obtained from the patient with SARS. Histopathologic examination of the brain tissue was performed using the methods of immunohistochemistry analysis and double immunofluorescence staining. Fifteen cytokines and chemokines were detected in the blood of the patient with SARS by means of a bead-based multiassay system. Results. A fragment specific for SARS human coronavirus was amplified from cultures of the brain suspension, and transmission electronic microscopy revealed the presence of an enveloped virus morphologically compatible with a coronavirus isolated in the cultures. Pathologic examination of the brain tissue revealed necrosis of neuron cells and broad hyperplasia of gliocytes. Immunostaining demonstrated that monokine induced by interferon-γ (Mig) was expressed in gliocytes with the infiltration of CD68<sup>+</sup>
 monocytes/macrophages and CD3<sup>+</sup>
 T lymphocytes in the brain mesenchyme. Cytokine/chemokine assay revealed that levels of interferon-γ-inducible protein 10 and Mig in the blood were highly elevated, although the levels of other cytokines and chemokines were close to normal. Conclusions. This study provides direct evidence that SARS human coronavirus is capable of infecting the central nervous system, and that Mig might be involved in the brain immunopathology of SARS.</EA>
<CC>002B05C02C</CC>
<FD>Syndrome respiratoire aigu sévère; Encéphale; Cerveau; Chimiokine; Coronavirus; Pathogénie</FD>
<FG>Virose; Infection; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Poumon pathologie</FG>
<ED>Severe acute respiratory syndrome; Encephalon; Brain; Chemokine; Coronavirus; Pathogenesis</ED>
<EG>Viral disease; Infection; Coronaviridae; Nidovirales; Virus; Respiratory disease; Lung disease</EG>
<SD>Síndrome respiratorio agudo severo; Encéfalo; Cerebro; Quimioquina; Coronavirus; Patogenia</SD>
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Detection of severe acute respiratory syndrome coronavirus in the brain : Potential role of the chemokine mig in pathogenesis

Detection of severe acute respiratory syndrome coronavirus in the brain : Potential role of the chemokine mig in pathogenesis

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