SrasV1, Ncbi, Merge, bibRecord, 002D40

Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.

Identifieur interne : 002D40 ( Ncbi/Merge ); précédent : 002D39; suivant : 002D41

Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.

Auteurs : Peng Zhou [Singapour] ; Yok Teng Chionh [Singapour] ; Sergio Erdal Irac [Singapour] ; Matae Ahn [Singapour] ; Justin Han Jia Ng [Singapour] ; Even Fossum [Norvège] ; Bjarne Bogen [Norvège] ; Florent Ginhoux [Singapour] ; Aaron T. Irving [Singapour] ; Charles-Antoine Dutertre [Singapour] ; Lin-Fa Wang [Singapour]

Source :

Scientific reports [ 2045-2322 ] ; 2016.

RBID : pubmed:27934903

Descripteurs français

KwdFr :
- Animaux, Cellules dendritiques (immunologie), Cellules dendritiques (métabolisme), Chiroptera (immunologie), Chiroptera (métabolisme), Cytokines (métabolisme), Immunité, Immunité innée, Immunophénotypage, Lymphocytes T (immunologie), Lymphocytes T (métabolisme), Macrophages (immunologie), Macrophages (métabolisme), Marqueurs biologiques, Phagocytose, Phénotype.
MESH :
- immunologie : Cellules dendritiques, Chiroptera, Lymphocytes T, Macrophages.
- métabolisme : Cellules dendritiques, Chiroptera, Cytokines, Lymphocytes T, Macrophages.
- Animaux, Immunité, Immunité innée, Immunophénotypage, Marqueurs biologiques, Phagocytose, Phénotype.

English descriptors

KwdEn :
- Animals, Biomarkers, Chiroptera (immunology), Chiroptera (metabolism), Cytokines (metabolism), Dendritic Cells (immunology), Dendritic Cells (metabolism), Immunity, Immunity, Innate, Immunophenotyping, Macrophages (immunology), Macrophages (metabolism), Phagocytosis, Phenotype, T-Lymphocytes (immunology), T-Lymphocytes (metabolism).
MESH :
- chemical , metabolism : Cytokines.
- chemical : Biomarkers.
- immunology : Chiroptera, Dendritic Cells, Macrophages, T-Lymphocytes.
- metabolism : Chiroptera, Dendritic Cells, Macrophages, T-Lymphocytes.
- Animals, Immunity, Immunity, Innate, Immunophenotyping, Phagocytosis, Phenotype.

Abstract

Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.

DOI: 10.1038/srep38597
PubMed: 27934903

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 000B78
to stream PubMed, to step Curation: 000B78
to stream PubMed, to step Checkpoint: 000B72

Links to Exploration step

pubmed:27934903

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.</title>
<author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chionh, Yok Teng" sort="Chionh, Yok Teng" uniqKey="Chionh Y" first="Yok Teng" last="Chionh">Yok Teng Chionh</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Irac, Sergio Erdal" sort="Irac, Sergio Erdal" uniqKey="Irac S" first="Sergio Erdal" last="Irac">Sergio Erdal Irac</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ahn, Matae" sort="Ahn, Matae" uniqKey="Ahn M" first="Matae" last="Ahn">Matae Ahn</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jia Ng, Justin Han" sort="Jia Ng, Justin Han" uniqKey="Jia Ng J" first="Justin Han" last="Jia Ng">Justin Han Jia Ng</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fossum, Even" sort="Fossum, Even" uniqKey="Fossum E" first="Even" last="Fossum">Even Fossum</name>
<affiliation wicri:level="1"><nlm:affiliation>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo, Norway.</nlm:affiliation>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bogen, Bjarne" sort="Bogen, Bjarne" uniqKey="Bogen B" first="Bjarne" last="Bogen">Bjarne Bogen</name>
<affiliation wicri:level="1"><nlm:affiliation>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo, Norway.</nlm:affiliation>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ginhoux, Florent" sort="Ginhoux, Florent" uniqKey="Ginhoux F" first="Florent" last="Ginhoux">Florent Ginhoux</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Irving, Aaron T" sort="Irving, Aaron T" uniqKey="Irving A" first="Aaron T" last="Irving">Aaron T. Irving</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dutertre, Charles Antoine" sort="Dutertre, Charles Antoine" uniqKey="Dutertre C" first="Charles-Antoine" last="Dutertre">Charles-Antoine Dutertre</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:27934903</idno>
<idno type="pmid">27934903</idno>
<idno type="doi">10.1038/srep38597</idno>
<idno type="wicri:Area/PubMed/Corpus">000B78</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000B78</idno>
<idno type="wicri:Area/PubMed/Curation">000B78</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000B78</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000B72</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000B72</idno>
<idno type="wicri:Area/Ncbi/Merge">002D40</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.</title>
<author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chionh, Yok Teng" sort="Chionh, Yok Teng" uniqKey="Chionh Y" first="Yok Teng" last="Chionh">Yok Teng Chionh</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Irac, Sergio Erdal" sort="Irac, Sergio Erdal" uniqKey="Irac S" first="Sergio Erdal" last="Irac">Sergio Erdal Irac</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ahn, Matae" sort="Ahn, Matae" uniqKey="Ahn M" first="Matae" last="Ahn">Matae Ahn</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jia Ng, Justin Han" sort="Jia Ng, Justin Han" uniqKey="Jia Ng J" first="Justin Han" last="Jia Ng">Justin Han Jia Ng</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fossum, Even" sort="Fossum, Even" uniqKey="Fossum E" first="Even" last="Fossum">Even Fossum</name>
<affiliation wicri:level="1"><nlm:affiliation>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo, Norway.</nlm:affiliation>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bogen, Bjarne" sort="Bogen, Bjarne" uniqKey="Bogen B" first="Bjarne" last="Bogen">Bjarne Bogen</name>
<affiliation wicri:level="1"><nlm:affiliation>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo, Norway.</nlm:affiliation>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ginhoux, Florent" sort="Ginhoux, Florent" uniqKey="Ginhoux F" first="Florent" last="Ginhoux">Florent Ginhoux</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Irving, Aaron T" sort="Irving, Aaron T" uniqKey="Irving A" first="Aaron T" last="Irving">Aaron T. Irving</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dutertre, Charles Antoine" sort="Dutertre, Charles Antoine" uniqKey="Dutertre C" first="Charles-Antoine" last="Dutertre">Charles-Antoine Dutertre</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore</wicri:regionArea>
<wicri:noRegion>169857 Singapore</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Biomarkers</term>
<term>Chiroptera (immunology)</term>
<term>Chiroptera (metabolism)</term>
<term>Cytokines (metabolism)</term>
<term>Dendritic Cells (immunology)</term>
<term>Dendritic Cells (metabolism)</term>
<term>Immunity</term>
<term>Immunity, Innate</term>
<term>Immunophenotyping</term>
<term>Macrophages (immunology)</term>
<term>Macrophages (metabolism)</term>
<term>Phagocytosis</term>
<term>Phenotype</term>
<term>T-Lymphocytes (immunology)</term>
<term>T-Lymphocytes (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules dendritiques (immunologie)</term>
<term>Cellules dendritiques (métabolisme)</term>
<term>Chiroptera (immunologie)</term>
<term>Chiroptera (métabolisme)</term>
<term>Cytokines (métabolisme)</term>
<term>Immunité</term>
<term>Immunité innée</term>
<term>Immunophénotypage</term>
<term>Lymphocytes T (immunologie)</term>
<term>Lymphocytes T (métabolisme)</term>
<term>Macrophages (immunologie)</term>
<term>Macrophages (métabolisme)</term>
<term>Marqueurs biologiques</term>
<term>Phagocytose</term>
<term>Phénotype</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biomarkers</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cellules dendritiques</term>
<term>Chiroptera</term>
<term>Lymphocytes T</term>
<term>Macrophages</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Chiroptera</term>
<term>Dendritic Cells</term>
<term>Macrophages</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chiroptera</term>
<term>Dendritic Cells</term>
<term>Macrophages</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules dendritiques</term>
<term>Chiroptera</term>
<term>Cytokines</term>
<term>Lymphocytes T</term>
<term>Macrophages</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Immunity</term>
<term>Immunity, Innate</term>
<term>Immunophenotyping</term>
<term>Phagocytosis</term>
<term>Phenotype</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Immunité</term>
<term>Immunité innée</term>
<term>Immunophénotypage</term>
<term>Marqueurs biologiques</term>
<term>Phagocytose</term>
<term>Phénotype</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27934903</PMID>
<DateCompleted><Year>2018</Year>
<Month>06</Month>
<Day>06</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>6</Volume>
<PubDate><Year>2016</Year>
<Month>12</Month>
<Day>09</Day>
</PubDate>
</JournalIssue>
<Title>Scientific reports</Title>
<ISOAbbreviation>Sci Rep</ISOAbbreviation>
</Journal>
<ArticleTitle>Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.</ArticleTitle>
<Pagination><MedlinePgn>38597</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/srep38597</ELocationID>
<Abstract><AbstractText>Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Peng</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chionh</LastName>
<ForeName>Yok Teng</ForeName>
<Initials>YT</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Irac</LastName>
<ForeName>Sergio Erdal</ForeName>
<Initials>SE</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ahn</LastName>
<ForeName>Matae</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jia Ng</LastName>
<ForeName>Justin Han</ForeName>
<Initials>JH</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fossum</LastName>
<ForeName>Even</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo, Norway.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bogen</LastName>
<ForeName>Bjarne</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>K.G. Jebsen Center for Research on Influenza Vaccines, Oslo University Hospital, University of Oslo, 0027 Oslo, Norway.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Center for Immune Regulation, Institute of Immunology, Oslo University Hospital Rikshospitalet, University of Oslo, 0424 Oslo, Norway.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ginhoux</LastName>
<ForeName>Florent</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, BIOPOLIS, 138648 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Irving</LastName>
<ForeName>Aaron T</ForeName>
<Initials>AT</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dutertre</LastName>
<ForeName>Charles-Antoine</ForeName>
<Initials>CA</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, BIOPOLIS, 138648 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Lin-Fa</ForeName>
<Initials>LF</Initials>
<AffiliationInfo><Affiliation>Programme in Emerging Infectious Disease, Duke-NUS Medical School, 8 College Road, 169857 Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>12</Month>
<Day>09</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Sci Rep</MedlineTA>
<NlmUniqueID>101563288</NlmUniqueID>
<ISSNLinking>2045-2322</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016207">Cytokines</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002685" MajorTopicYN="N">Chiroptera</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003713" MajorTopicYN="N">Dendritic Cells</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007109" MajorTopicYN="Y">Immunity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016130" MajorTopicYN="N">Immunophenotyping</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008264" MajorTopicYN="N">Macrophages</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010587" MajorTopicYN="N">Phagocytosis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>03</Month>
<Day>11</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>11</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>12</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2016</Year>
<Month>12</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>6</Month>
<Day>7</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">27934903</ArticleId>
<ArticleId IdType="pii">srep38597</ArticleId>
<ArticleId IdType="doi">10.1038/srep38597</ArticleId>
<ArticleId IdType="pmc">PMC5146944</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Nature. 2005 Dec 1;438(7068):575-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16319873</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Immunol. 2013;120:69-103</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24070381</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virus Res. 2009 Oct;145(1):54-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19540275</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Immunol. 1995;13:251-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7612223</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Aug 06;9(8):e103875</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25100081</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunity. 2013 May 23;38(5):970-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23706669</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2009 Dec 11;4(12):e8266</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20011515</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Genet. 2013 Oct;29(10):569-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23810203</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Comp Pathol. 2007 May;136(4):266-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17498518</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Immunol. 2016 Jan;17(1):34-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26681460</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Virol. 2011 Dec;1(6):649-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22440923</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 1998 Jul;16(11-12):1122-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9682368</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2000 Aug;81(Pt 8):1927-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10900029</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Immunol. 2015 Feb;45(2):624-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25410055</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Microbiol Rev. 2006 Jul;19(3):531-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16847084</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Scand J Immunol. 2012 Apr;75(4):379-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21955209</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 1996 Oct-Dec;2(4):321-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8969248</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 1996 Nov 1;184(5):1953-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8920882</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2001 May 1;29(9):e45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11328886</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Immunol. 2005 Nov;6(11):1123-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16200070</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2011;6(7):e22488</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21811620</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2011 Nov;204 Suppl 3:S840-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21987760</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 2010 Nov 22;207(12):2703-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20975040</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2011 Mar 1;186(5):3138-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21278349</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Nov 28;503(7477):535-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24172901</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Cell Dev Biol. 2015 May;41:9-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25957517</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2015 Dec;21(12):1508-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26552008</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2013 Jan 25;339(6118):456-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23258410</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunity. 2000 Nov;13(5):715-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11114383</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunol Rev. 2007 Oct;219:187-203</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17850490</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Dec;87(24):13889-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24067951</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2011;6(11):e28131</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22140523</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Immunol. 2000;18:767-811</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10837075</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbes Infect. 2002 Feb;4(2):145-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11880045</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunol Rev. 2005 Oct;207:191-205</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16181337</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Immunol. 2014 Jun;14(6):392-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24854589</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Immunol. 2015;33:643-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25861979</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 1992 Dec 1;176(6):1693-702</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1460426</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 1988 Nov 15;141(10):3383-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3053898</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Immunol. 2014 Sep-Oct;291(1-2):3-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25262488</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Norvège</li>
<li>Singapour</li>
</country>
<region><li>Østlandet</li>
</region>
<settlement><li>Oslo</li>
</settlement>
</list>
<tree><country name="Singapour"><noRegion><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name>
</noRegion>
<name sortKey="Ahn, Matae" sort="Ahn, Matae" uniqKey="Ahn M" first="Matae" last="Ahn">Matae Ahn</name>
<name sortKey="Chionh, Yok Teng" sort="Chionh, Yok Teng" uniqKey="Chionh Y" first="Yok Teng" last="Chionh">Yok Teng Chionh</name>
<name sortKey="Dutertre, Charles Antoine" sort="Dutertre, Charles Antoine" uniqKey="Dutertre C" first="Charles-Antoine" last="Dutertre">Charles-Antoine Dutertre</name>
<name sortKey="Ginhoux, Florent" sort="Ginhoux, Florent" uniqKey="Ginhoux F" first="Florent" last="Ginhoux">Florent Ginhoux</name>
<name sortKey="Irac, Sergio Erdal" sort="Irac, Sergio Erdal" uniqKey="Irac S" first="Sergio Erdal" last="Irac">Sergio Erdal Irac</name>
<name sortKey="Irving, Aaron T" sort="Irving, Aaron T" uniqKey="Irving A" first="Aaron T" last="Irving">Aaron T. Irving</name>
<name sortKey="Jia Ng, Justin Han" sort="Jia Ng, Justin Han" uniqKey="Jia Ng J" first="Justin Han" last="Jia Ng">Justin Han Jia Ng</name>
<name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name>
</country>
<country name="Norvège"><region name="Østlandet"><name sortKey="Fossum, Even" sort="Fossum, Even" uniqKey="Fossum E" first="Even" last="Fossum">Even Fossum</name>
</region>
<name sortKey="Bogen, Bjarne" sort="Bogen, Bjarne" uniqKey="Bogen B" first="Bjarne" last="Bogen">Bjarne Bogen</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002D40 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 002D40 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Ncbi
   |étape=   Merge
   |type=    RBID
   |clé=     pubmed:27934903
   |texte=   Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i   -Sk "pubmed:27934903" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.

Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki