Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.
Identifieur interne : 002D40 ( Ncbi/Merge ); précédent : 002D39; suivant : 002D41Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages.
Auteurs : Peng Zhou [Singapour] ; Yok Teng Chionh [Singapour] ; Sergio Erdal Irac [Singapour] ; Matae Ahn [Singapour] ; Justin Han Jia Ng [Singapour] ; Even Fossum [Norvège] ; Bjarne Bogen [Norvège] ; Florent Ginhoux [Singapour] ; Aaron T. Irving [Singapour] ; Charles-Antoine Dutertre [Singapour] ; Lin-Fa Wang [Singapour]Source :
- Scientific reports [ 2045-2322 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Cellules dendritiques (immunologie), Cellules dendritiques (métabolisme), Chiroptera (immunologie), Chiroptera (métabolisme), Cytokines (métabolisme), Immunité, Immunité innée, Immunophénotypage, Lymphocytes T (immunologie), Lymphocytes T (métabolisme), Macrophages (immunologie), Macrophages (métabolisme), Marqueurs biologiques, Phagocytose, Phénotype.
- MESH :
- immunologie : Cellules dendritiques, Chiroptera, Lymphocytes T, Macrophages.
- métabolisme : Cellules dendritiques, Chiroptera, Cytokines, Lymphocytes T, Macrophages.
- Animaux, Immunité, Immunité innée, Immunophénotypage, Marqueurs biologiques, Phagocytose, Phénotype.
English descriptors
- KwdEn :
- Animals, Biomarkers, Chiroptera (immunology), Chiroptera (metabolism), Cytokines (metabolism), Dendritic Cells (immunology), Dendritic Cells (metabolism), Immunity, Immunity, Innate, Immunophenotyping, Macrophages (immunology), Macrophages (metabolism), Phagocytosis, Phenotype, T-Lymphocytes (immunology), T-Lymphocytes (metabolism).
- MESH :
- chemical , metabolism : Cytokines.
- chemical : Biomarkers.
- immunology : Chiroptera, Dendritic Cells, Macrophages, T-Lymphocytes.
- metabolism : Chiroptera, Dendritic Cells, Macrophages, T-Lymphocytes.
- Animals, Immunity, Immunity, Innate, Immunophenotyping, Phagocytosis, Phenotype.
Abstract
Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.
DOI: 10.1038/srep38597
PubMed: 27934903
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pubmed:27934903Le document en format XML
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<term>Dendritic Cells (immunology)</term>
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<term>Cytokines (métabolisme)</term>
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<term>Dendritic Cells</term>
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<term>T-Lymphocytes</term>
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<front><div type="abstract" xml:lang="en">Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.</div>
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<Abstract><AbstractText>Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.</AbstractText>
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<Initials>B</Initials>
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