[Construction and screening of human phage antibody library against SARS virus].
Identifieur interne : 000C94 ( Ncbi/Merge ); précédent : 000C93; suivant : 000C95[Construction and screening of human phage antibody library against SARS virus].
Auteurs : Xiao-Ping Kang [République populaire de Chine] ; Wei Xiong ; Guo-Hui Chang ; Qing-Yu ZhuSource :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Anticorps antiviraux, Bactériophages.
- immunologie : Anticorps antiviraux, Antigènes viraux, Virus du SRAS.
- Animaux, Banque de peptides, Bovins, Humains, Réaction de polymérisation en chaîne, Spécificité des anticorps.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Antibodies, Viral.
- chemical , immunology : Antibodies, Viral, Antigens, Viral.
- genetics : Bacteriophages.
- immunology : SARS Virus.
- Animals, Antibody Specificity, Cattle, Humans, Peptide Library, Polymerase Chain Reaction.
Abstract
To construct human phage antibody library against SARS virus.
PubMed: 15629082
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pubmed:15629082Le document en format XML
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Hui" sort="Chang, Guo Hui" uniqKey="Chang G" first="Guo-Hui" last="Chang">Guo-Hui Chang</name></author><author><name sortKey="Zhu, Qing Yu" sort="Zhu, Qing Yu" uniqKey="Zhu Q" first="Qing-Yu" last="Zhu">Qing-Yu Zhu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15629082</idno><idno type="pmid">15629082</idno><idno type="wicri:Area/PubMed/Corpus">002971</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002971</idno><idno type="wicri:Area/PubMed/Curation">002971</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002971</idno><idno type="wicri:Area/PubMed/Checkpoint">002384</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002384</idno><idno type="wicri:Area/Ncbi/Merge">000C94</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Construction and screening of human phage antibody library against SARS virus].</title><author><name sortKey="Kang, Xiao Ping" sort="Kang, Xiao Ping" uniqKey="Kang X" first="Xiao-Ping" last="Kang">Xiao-Ping Kang</name><affiliation wicri:level="1"><nlm:affiliation>The Army Center of Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Army Center of Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name></author><author><name sortKey="Chang, Guo Hui" sort="Chang, Guo Hui" uniqKey="Chang G" first="Guo-Hui" last="Chang">Guo-Hui Chang</name></author><author><name sortKey="Zhu, Qing Yu" sort="Zhu, Qing Yu" uniqKey="Zhu Q" first="Qing-Yu" last="Zhu">Qing-Yu Zhu</name></author></analytic><series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title><idno type="ISSN">1007-8738</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (genetics)</term><term>Antibodies, Viral (immunology)</term><term>Antibody Specificity</term><term>Antigens, Viral (immunology)</term><term>Bacteriophages (genetics)</term><term>Cattle</term><term>Humans</term><term>Peptide Library</term><term>Polymerase Chain Reaction</term><term>SARS Virus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (génétique)</term><term>Anticorps antiviraux (immunologie)</term><term>Antigènes viraux (immunologie)</term><term>Bactériophages (génétique)</term><term>Banque de peptides</term><term>Bovins</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term><term>Spécificité des anticorps</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteriophages</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Anticorps antiviraux</term><term>Bactériophages</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Antigènes viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Specificity</term><term>Cattle</term><term>Humans</term><term>Peptide Library</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Banque de peptides</term><term>Bovins</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term><term>Spécificité des anticorps</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To construct human phage antibody library against SARS virus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15629082</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2005</Year><Month>01</Month><Day>04</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-8738</ISSN><JournalIssue CitedMedium="Print"><Volume>21</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</Title><ISOAbbreviation>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Construction and screening of human phage antibody library against SARS virus].</ArticleTitle><Pagination><MedlinePgn>46-9</MedlinePgn></Pagination><Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To construct human phage antibody library against SARS virus.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Human Fab genes were amplified by RT-PCR from lymphocytes of a convalescent SARS patient and cloned into vector pComb3 to construct phage antibody library. Antibodies against SARS virus were screened by biopanning with immobilized virus antigen. The binding specificity of phage antibody to SARS virus was detected by ELISA.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">13 Ig genes were obtained by PCR. A human phage antibody library consisting of 1.3 x 10(6) clones was constructed by electrotransformation and 60 percent of the clones contained Fab genes. After three rounds of panning with SARS virus antigen, phage antibodies against SARS virus were specifically enriched. ELISA detection verified 10 positive phage antibodies that were highly specific for SARS virus.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Human phage antibody library against SARS virus has been constructed successfully, from which 10 anti-SARS virus antibodies were obtained.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Xiao-Ping</ForeName><Initials>XP</Initials><AffiliationInfo><Affiliation>The Army Center of Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiong</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Guo-Hui</ForeName><Initials>GH</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Qing-Yu</ForeName><Initials>QY</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</MedlineTA><NlmUniqueID>101139110</NlmUniqueID><ISSNLinking>1007-8738</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019151">Peptide Library</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000918" MajorTopicYN="N">Antibody Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001435" MajorTopicYN="N">Bacteriophages</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002417" MajorTopicYN="N">Cattle</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019151" MajorTopicYN="Y">Peptide Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>1</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>4</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>1</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15629082</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chang, Guo Hui" sort="Chang, Guo Hui" uniqKey="Chang G" first="Guo-Hui" last="Chang">Guo-Hui Chang</name><name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name><name sortKey="Zhu, Qing Yu" sort="Zhu, Qing Yu" uniqKey="Zhu Q" first="Qing-Yu" last="Zhu">Qing-Yu Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Kang, Xiao Ping" sort="Kang, Xiao Ping" uniqKey="Kang X" first="Xiao-Ping" last="Kang">Xiao-Ping Kang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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