[Preparation of specific monoclonal antibody against nucleocapsid protein of SARS coronavirus].
Identifieur interne : 000C95 ( Ncbi/Merge ); précédent : 000C94; suivant : 000C96[Preparation of specific monoclonal antibody against nucleocapsid protein of SARS coronavirus].
Auteurs : Ping Wang [République populaire de Chine] ; Qing-Sheng Huang ; Xiao-Ping Xue ; Ying-Feng Lei ; Hai-Tao Wang ; Jun-Ping Ren ; Tian-Bing Ding ; Zhi-Kai XuSource :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2005.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Anticorps monoclonaux (immunologie), Clonage moléculaire, Escherichia coli (génétique), Expression des gènes, Humains, Protéines nucléocapside (biosynthèse), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Souris, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Protéines nucléocapside.
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Escherichia coli, Protéines nucléocapside.
- immunologie : Anticorps monoclonaux, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- Analyse de séquence d'ADN, Animaux, Clonage moléculaire, Expression des gènes, Humains, Souris.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Cloning, Molecular, Escherichia coli (genetics), Gene Expression, Humans, Mice, Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), SARS Virus (immunology), Sequence Analysis, DNA, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , biosynthesis : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Monoclonal, Nucleocapsid Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : Escherichia coli.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- Animals, Cloning, Molecular, Gene Expression, Humans, Mice, Sequence Analysis, DNA.
Abstract
To express nucleocapsid(N) protein of SARS coronavirus and produce monoclonal antibody(mAb) to N protein.
PubMed: 15629083
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pubmed:15629083Le document en format XML
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sort="Lei, Ying Feng" uniqKey="Lei Y" first="Ying-Feng" last="Lei">Ying-Feng Lei</name></author><author><name sortKey="Wang, Hai Tao" sort="Wang, Hai Tao" uniqKey="Wang H" first="Hai-Tao" last="Wang">Hai-Tao Wang</name></author><author><name sortKey="Ren, Jun Ping" sort="Ren, Jun Ping" uniqKey="Ren J" first="Jun-Ping" last="Ren">Jun-Ping Ren</name></author><author><name sortKey="Ding, Tian Bing" sort="Ding, Tian Bing" uniqKey="Ding T" first="Tian-Bing" last="Ding">Tian-Bing Ding</name></author><author><name sortKey="Xu, Zhi Kai" sort="Xu, Zhi Kai" uniqKey="Xu Z" first="Zhi-Kai" last="Xu">Zhi-Kai Xu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15629083</idno><idno type="pmid">15629083</idno><idno type="wicri:Area/PubMed/Corpus">002970</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002970</idno><idno 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710032</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Qing Sheng" sort="Huang, Qing Sheng" uniqKey="Huang Q" first="Qing-Sheng" last="Huang">Qing-Sheng Huang</name></author><author><name sortKey="Xue, Xiao Ping" sort="Xue, Xiao Ping" uniqKey="Xue X" first="Xiao-Ping" last="Xue">Xiao-Ping Xue</name></author><author><name sortKey="Lei, Ying Feng" sort="Lei, Ying Feng" uniqKey="Lei Y" first="Ying-Feng" last="Lei">Ying-Feng Lei</name></author><author><name sortKey="Wang, Hai Tao" sort="Wang, Hai Tao" uniqKey="Wang H" first="Hai-Tao" last="Wang">Hai-Tao Wang</name></author><author><name sortKey="Ren, Jun Ping" sort="Ren, Jun Ping" uniqKey="Ren J" first="Jun-Ping" last="Ren">Jun-Ping Ren</name></author><author><name sortKey="Ding, Tian Bing" sort="Ding, Tian Bing" uniqKey="Ding T" first="Tian-Bing" last="Ding">Tian-Bing Ding</name></author><author><name sortKey="Xu, Zhi Kai" sort="Xu, Zhi Kai" uniqKey="Xu Z" first="Zhi-Kai" last="Xu">Zhi-Kai Xu</name></author></analytic><series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title><idno type="ISSN">1007-8738</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Cloning, Molecular</term><term>Escherichia coli (genetics)</term><term>Gene Expression</term><term>Humans</term><term>Mice</term><term>Nucleocapsid Proteins (biosynthesis)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>Sequence Analysis, DNA</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Anticorps monoclonaux (immunologie)</term><term>Clonage moléculaire</term><term>Escherichia coli (génétique)</term><term>Expression des gènes</term><term>Humains</term><term>Protéines nucléocapside (biosynthèse)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Souris</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cloning, Molecular</term><term>Gene Expression</term><term>Humans</term><term>Mice</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Clonage moléculaire</term><term>Expression des gènes</term><term>Humains</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To express nucleocapsid(N) protein of SARS coronavirus and produce monoclonal antibody(mAb) to N protein.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15629083</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2005</Year><Month>01</Month><Day>04</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-8738</ISSN><JournalIssue CitedMedium="Print"><Volume>21</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</Title><ISOAbbreviation>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Preparation of specific monoclonal antibody against nucleocapsid protein of SARS coronavirus].</ArticleTitle><Pagination><MedlinePgn>50-2</MedlinePgn></Pagination><Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To express nucleocapsid(N) protein of SARS coronavirus and produce monoclonal antibody(mAb) to N protein.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">N protein gene was amplified by RT-PCR. After being confirmed by DNA sequencing, the gene was subcloned into prokaryotic expression vector. N protein expressed in E.coli was recovered from SDS-PAGE gel and served as immunogen in the preparation of the mAb.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">DNA sequencing confirmed that the amplified fragment was N protein gene. SDS-PAGE analysis showed that the M(r) of the expressed protein was approximately 43 kd. This expressed protein could be further confirmed in Western blot by using serum from a convalescent SARS patient as primary antibody. Western blot analysis proved that three mAbs obtained could react specifically to the recombinant N protein.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The prepared recombinant N protein and mAbs against N protein lay the foundation for further development of early diagnosis assays for SARS coronavirus infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ping</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Qing-Sheng</ForeName><Initials>QS</Initials></Author><Author ValidYN="Y"><LastName>Xue</LastName><ForeName>Xiao-Ping</ForeName><Initials>XP</Initials></Author><Author ValidYN="Y"><LastName>Lei</LastName><ForeName>Ying-Feng</ForeName><Initials>YF</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hai-Tao</ForeName><Initials>HT</Initials></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Jun-Ping</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Tian-Bing</ForeName><Initials>TB</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Zhi-Kai</ForeName><Initials>ZK</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</MedlineTA><NlmUniqueID>101139110</NlmUniqueID><ISSNLinking>1007-8738</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>1</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>4</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>1</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15629083</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Ding, Tian Bing" sort="Ding, Tian Bing" uniqKey="Ding T" first="Tian-Bing" last="Ding">Tian-Bing Ding</name><name sortKey="Huang, Qing Sheng" sort="Huang, Qing Sheng" uniqKey="Huang Q" first="Qing-Sheng" last="Huang">Qing-Sheng Huang</name><name sortKey="Lei, Ying Feng" sort="Lei, Ying Feng" uniqKey="Lei Y" first="Ying-Feng" last="Lei">Ying-Feng Lei</name><name sortKey="Ren, Jun Ping" sort="Ren, Jun Ping" uniqKey="Ren J" first="Jun-Ping" last="Ren">Jun-Ping Ren</name><name sortKey="Wang, Hai Tao" sort="Wang, Hai Tao" uniqKey="Wang H" first="Hai-Tao" last="Wang">Hai-Tao Wang</name><name sortKey="Xu, Zhi Kai" sort="Xu, Zhi Kai" uniqKey="Xu Z" first="Zhi-Kai" last="Xu">Zhi-Kai Xu</name><name sortKey="Xue, Xiao Ping" sort="Xue, Xiao Ping" uniqKey="Xue X" first="Xiao-Ping" last="Xue">Xiao-Ping Xue</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Ping" sort="Wang, Ping" uniqKey="Wang P" first="Ping" last="Wang">Ping Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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