Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays.
Identifieur interne : 000542 ( Ncbi/Checkpoint ); précédent : 000541; suivant : 000543Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays.
Auteurs : Leo L M. Poon [Hong Kong] ; Kwok Hung Chan ; On Kei Wong ; Timothy K W. Cheung ; Iris Ng ; Bojian Zheng ; Wing Hong Seto ; Kwok Yung Yuen ; Yi Guan ; Joseph S M. PeirisSource :
- Clinical chemistry [ 0009-9147 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Fosse nasale, Fèces, Pharynx, Syndrome respiratoire aigu sévère.
- Humains, Prélèvement biologique, RT-PCR.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Feces, Nasal Cavity, Pharynx, Severe Acute Respiratory Syndrome.
- Humans, Reverse Transcriptase Polymerase Chain Reaction, Specimen Handling.
Abstract
A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens.
DOI: 10.1373/clinchem.2003.023663
PubMed: 14709637
Affiliations:
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pubmed:14709637Le document en format XML
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Cheung</name></author><author><name sortKey="Ng, Iris" sort="Ng, Iris" uniqKey="Ng I" first="Iris" last="Ng">Iris Ng</name></author><author><name sortKey="Zheng, Bojian" sort="Zheng, Bojian" uniqKey="Zheng B" first="Bojian" last="Zheng">Bojian Zheng</name></author><author><name sortKey="Seto, Wing Hong" sort="Seto, Wing Hong" uniqKey="Seto W" first="Wing Hong" last="Seto">Wing Hong Seto</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok Yung" last="Yuen">Kwok Yung Yuen</name></author><author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name></author><author><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S M" last="Peiris">Joseph S M. 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Cheung</name></author><author><name sortKey="Ng, Iris" sort="Ng, Iris" uniqKey="Ng I" first="Iris" last="Ng">Iris Ng</name></author><author><name sortKey="Zheng, Bojian" sort="Zheng, Bojian" uniqKey="Zheng B" first="Bojian" last="Zheng">Bojian Zheng</name></author><author><name sortKey="Seto, Wing Hong" sort="Seto, Wing Hong" uniqKey="Seto W" first="Wing Hong" last="Seto">Wing Hong Seto</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok Yung" last="Yuen">Kwok Yung Yuen</name></author><author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name></author><author><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S M" last="Peiris">Joseph S M. 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