Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays.
Identifieur interne : 000542 ( Ncbi/Curation ); précédent : 000541; suivant : 000543Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays.
Auteurs : Leo L M. Poon [Hong Kong] ; Kwok Hung Chan ; On Kei Wong ; Timothy K W. Cheung ; Iris Ng ; Bojian Zheng ; Wing Hong Seto ; Kwok Yung Yuen ; Yi Guan ; Joseph S M. PeirisSource :
- Clinical chemistry [ 0009-9147 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Fosse nasale, Fèces, Pharynx, Syndrome respiratoire aigu sévère.
- Humains, Prélèvement biologique, RT-PCR.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Feces, Nasal Cavity, Pharynx, Severe Acute Respiratory Syndrome.
- Humans, Reverse Transcriptase Polymerase Chain Reaction, Specimen Handling.
Abstract
A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens.
DOI: 10.1373/clinchem.2003.023663
PubMed: 14709637
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pubmed:14709637Le document en format XML
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<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name>
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<author><name sortKey="Wong, On Kei" sort="Wong, On Kei" uniqKey="Wong O" first="On Kei" last="Wong">On Kei Wong</name>
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<author><name sortKey="Cheung, Timothy K W" sort="Cheung, Timothy K W" uniqKey="Cheung T" first="Timothy K W" last="Cheung">Timothy K W. Cheung</name>
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<author><name sortKey="Ng, Iris" sort="Ng, Iris" uniqKey="Ng I" first="Iris" last="Ng">Iris Ng</name>
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<author><name sortKey="Zheng, Bojian" sort="Zheng, Bojian" uniqKey="Zheng B" first="Bojian" last="Zheng">Bojian Zheng</name>
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<series><title level="j">Clinical chemistry</title>
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<term>Reverse Transcriptase Polymerase Chain Reaction</term>
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<term>Pharynx (virologie)</term>
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<front><div type="abstract" xml:lang="en">A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens.</div>
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