Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays.
Identifieur interne : 000542 ( Ncbi/Merge ); précédent : 000541; suivant : 000543Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays.
Auteurs : Leo L M. Poon [Hong Kong] ; Kwok Hung Chan ; On Kei Wong ; Timothy K W. Cheung ; Iris Ng ; Bojian Zheng ; Wing Hong Seto ; Kwok Yung Yuen ; Yi Guan ; Joseph S M. PeirisSource :
- Clinical chemistry [ 0009-9147 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Fosse nasale, Fèces, Pharynx, Syndrome respiratoire aigu sévère.
- Humains, Prélèvement biologique, RT-PCR.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Feces, Nasal Cavity, Pharynx, Severe Acute Respiratory Syndrome.
- Humans, Reverse Transcriptase Polymerase Chain Reaction, Specimen Handling.
Abstract
A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens.
DOI: 10.1373/clinchem.2003.023663
PubMed: 14709637
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Cheung</name></author><author><name sortKey="Ng, Iris" sort="Ng, Iris" uniqKey="Ng I" first="Iris" last="Ng">Iris Ng</name></author><author><name sortKey="Zheng, Bojian" sort="Zheng, Bojian" uniqKey="Zheng B" first="Bojian" last="Zheng">Bojian Zheng</name></author><author><name sortKey="Seto, Wing Hong" sort="Seto, Wing Hong" uniqKey="Seto W" first="Wing Hong" last="Seto">Wing Hong Seto</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok Yung" last="Yuen">Kwok Yung Yuen</name></author><author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name></author><author><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S M" last="Peiris">Joseph S M. Peiris</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:14709637</idno><idno type="pmid">14709637</idno><idno type="doi">10.1373/clinchem.2003.023663</idno><idno type="wicri:Area/PubMed/Corpus">003010</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003010</idno><idno type="wicri:Area/PubMed/Curation">003010</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003010</idno><idno type="wicri:Area/PubMed/Checkpoint">002D56</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D56</idno><idno type="wicri:Area/Ncbi/Merge">000542</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays.</title><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L M" last="Poon">Leo L M. Poon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong SAR. llmpoon@hkucc.hku.hk</nlm:affiliation><country wicri:rule="url">Hong Kong</country></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name></author><author><name sortKey="Wong, On Kei" sort="Wong, On Kei" uniqKey="Wong O" first="On Kei" last="Wong">On Kei Wong</name></author><author><name sortKey="Cheung, Timothy K W" sort="Cheung, Timothy K W" uniqKey="Cheung T" first="Timothy K W" last="Cheung">Timothy K W. Cheung</name></author><author><name sortKey="Ng, Iris" sort="Ng, Iris" uniqKey="Ng I" first="Iris" last="Ng">Iris Ng</name></author><author><name sortKey="Zheng, Bojian" sort="Zheng, Bojian" uniqKey="Zheng B" first="Bojian" last="Zheng">Bojian Zheng</name></author><author><name sortKey="Seto, Wing Hong" sort="Seto, Wing Hong" uniqKey="Seto W" first="Wing Hong" last="Seto">Wing Hong Seto</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok Yung" last="Yuen">Kwok Yung Yuen</name></author><author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name></author><author><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S M" last="Peiris">Joseph S M. Peiris</name></author></analytic><series><title level="j">Clinical chemistry</title><idno type="ISSN">0009-9147</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Feces (virology)</term><term>Humans</term><term>Nasal Cavity (virology)</term><term>Pharynx (virology)</term><term>RNA, Viral (analysis)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Specimen Handling</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Fosse nasale (virologie)</term><term>Fèces (virologie)</term><term>Humains</term><term>Pharynx (virologie)</term><term>Prélèvement biologique</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fosse nasale</term><term>Fèces</term><term>Pharynx</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term><term>Nasal Cavity</term><term>Pharynx</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Specimen Handling</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Prélèvement biologique</term><term>RT-PCR</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14709637</PMID><DateCompleted><Year>2004</Year><Month>02</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>04</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0009-9147</ISSN><JournalIssue CitedMedium="Print"><Volume>50</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Clinical chemistry</Title><ISOAbbreviation>Clin. Chem.</ISOAbbreviation></Journal><ArticleTitle>Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays.</ArticleTitle><Pagination><MedlinePgn>67-72</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">RNA samples isolated from nasopharyngeal aspirate (NPA; n = 170) and stool (n = 44) were reverse-transcribed and tested by our in-house conventional RT-PCR assay. We selected 98 NPA and 37 stool samples collected at different times after the onset of disease and tested them in a real-time quantitative RT-PCR specific for the open reading frame (ORF) 1b region of SARS CoV. Detection rates for the conventional and real-time quantitative RT-PCR assays were compared. To investigate the nature of viral RNA molecules in these clinical samples, we determined copy numbers of ORF 1b and nucleocapsid (N) gene sequences of SARS CoV.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The quantitative real-time RT-PCR assay was more sensitive than the conventional RT-PCR assay for detecting SARS CoV in samples collected early in the course of the disease. Real-time assays targeted at the ORF 1b region and the N gene revealed that copy numbers of ORF 1b and N gene sequences in clinical samples were similar.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">NPA and stool samples can be used for early diagnosis of SARS. The real-time quantitative RT-PCR assay for SARS CoV is potentially useful for early detection of SARS CoV. Our results suggest that genomic RNA is the predominant viral RNA species in clinical samples.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Poon</LastName><ForeName>Leo L M</ForeName><Initials>LL</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong SAR. llmpoon@hkucc.hku.hk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Kwok Hung</ForeName><Initials>KH</Initials></Author><Author ValidYN="Y"><LastName>Wong</LastName><ForeName>On Kei</ForeName><Initials>OK</Initials></Author><Author ValidYN="Y"><LastName>Cheung</LastName><ForeName>Timothy K W</ForeName><Initials>TK</Initials></Author><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>Iris</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Bojian</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Seto</LastName><ForeName>Wing Hong</ForeName><Initials>WH</Initials></Author><Author ValidYN="Y"><LastName>Yuen</LastName><ForeName>Kwok Yung</ForeName><Initials>KY</Initials></Author><Author ValidYN="Y"><LastName>Guan</LastName><ForeName>Yi</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Peiris</LastName><ForeName>Joseph S M</ForeName><Initials>JS</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Acronym>WT_</Acronym><Agency>Wellcome Trust</Agency><Country>United Kingdom</Country></Grant><Grant><GrantID>A195357</GrantID><Agency>PHS HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, 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MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013048" MajorTopicYN="N">Specimen Handling</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate 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IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Hong Kong</li></country></list><tree><noCountry><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok Hung" last="Chan">Kwok Hung Chan</name><name sortKey="Cheung, Timothy K W" sort="Cheung, Timothy K W" uniqKey="Cheung T" first="Timothy K W" last="Cheung">Timothy K W. Cheung</name><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name><name sortKey="Ng, Iris" sort="Ng, Iris" uniqKey="Ng I" first="Iris" last="Ng">Iris Ng</name><name sortKey="Peiris, Joseph S M" sort="Peiris, Joseph S M" uniqKey="Peiris J" first="Joseph S M" last="Peiris">Joseph S M. Peiris</name><name sortKey="Seto, Wing Hong" sort="Seto, Wing Hong" uniqKey="Seto W" first="Wing Hong" last="Seto">Wing Hong Seto</name><name sortKey="Wong, On Kei" sort="Wong, On Kei" uniqKey="Wong O" first="On Kei" last="Wong">On Kei Wong</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok Yung" last="Yuen">Kwok Yung Yuen</name><name sortKey="Zheng, Bojian" sort="Zheng, Bojian" uniqKey="Zheng B" first="Bojian" last="Zheng">Bojian Zheng</name></noCountry><country name="Hong Kong"><noRegion><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L M" last="Poon">Leo L M. 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