Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.
Identifieur interne : 004B33 ( Main/Merge ); précédent : 004B32; suivant : 004B34Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.
Auteurs : Eric C. Mossel [États-Unis] ; Cheng Huang ; Krishna Narayanan ; Shinji Makino ; Robert B. Tesh ; C J PetersSource :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Carboxypeptidases (génétique), Carboxypeptidases (physiologie), Cellules Caco-2, Cellules Vero, Chats, Cricetinae, Herpestidae, Humains, Lapins, Lignée cellulaire, Peptidyl-Dipeptidase A, Récepteurs viraux (génétique), Récepteurs viraux (physiologie), Réplication virale, Souris, Suidae, Tupaia, Virus du SRAS (croissance et développement), Virus du SRAS (physiologie), Visons.
- MESH :
- croissance et développement : Virus du SRAS.
- génétique : Carboxypeptidases, Récepteurs viraux.
- physiologie : Carboxypeptidases, Récepteurs viraux, Virus du SRAS.
- Animaux, Cellules Caco-2, Cellules Vero, Chats, Cricetinae, Herpestidae, Humains, Lapins, Lignée cellulaire, Peptidyl-Dipeptidase A, Réplication virale, Souris, Suidae, Tupaia, Visons.
English descriptors
- KwdEn :
- Animals, Caco-2 Cells, Carboxypeptidases (genetics), Carboxypeptidases (physiology), Cats, Cell Line, Chlorocebus aethiops, Cricetinae, Herpestidae, Humans, Mice, Mink, Peptidyl-Dipeptidase A, Rabbits, Receptors, Virus (genetics), Receptors, Virus (physiology), SARS Virus (growth & development), SARS Virus (physiology), Swine, Tupaia, Vero Cells, Virus Replication.
- MESH :
- chemical , genetics : Carboxypeptidases, Receptors, Virus.
- chemical , physiology : Carboxypeptidases, Receptors, Virus.
- growth & development : SARS Virus.
- physiology : SARS Virus.
- Animals, Caco-2 Cells, Cats, Cell Line, Chlorocebus aethiops, Cricetinae, Herpestidae, Humans, Mice, Mink, Peptidyl-Dipeptidase A, Rabbits, Swine, Tupaia, Vero Cells, Virus Replication.
Abstract
Of 30 cell lines and primary cells examined, productive severe acute respiratory syndrome coronavirus (Urbani strain) (SARS-CoV) infection after low-multiplicity inoculation was detected in only six: three African green monkey kidney epithelial cell lines (Vero, Vero E6, and MA104), a human colon epithelial line (CaCo-2), a porcine kidney epithelial line [PK(15)], and mink lung epithelial cells (Mv 1 Lu). SARS-CoV produced a lytic infection in Vero, Vero E6, and MA104 cells, but there was no visible cytopathic effect in Caco-2, Mv 1 Lu, or PK(15) cells. Multistep growth kinetics were identical in Vero E6 and MA104 cells, with maximum titer reached 24 h postinoculation (hpi). Virus titer was maximal 96 hpi in CaCo-2 cells, and virus was continually produced from infected CaCo-2 cells for at least 6 weeks after infection. CaCo-2 was the only human cell type of 13 tested that supported efficient SARS-CoV replication. Expression of the SARS-CoV receptor, angiotensin-converting enzyme 2 (ACE2), resulted in SARS-CoV replication in all refractory cell lines examined. Titers achieved were variable and dependent upon the method of ACE2 expression.
DOI: 10.1128/JVI.79.6.3846-3850.2005
PubMed: 15731278
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pubmed:15731278Le document en format XML
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Mossel</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology, Immunology, and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, CO 80523-1619, USA. ecmossel@colostate.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, Immunology, and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, CO 80523-1619</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name></author><author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name></author><author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name></author><author><name sortKey="Tesh, Robert B" sort="Tesh, Robert B" uniqKey="Tesh R" first="Robert B" last="Tesh">Robert B. 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Mossel</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology, Immunology, and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, CO 80523-1619, USA. ecmossel@colostate.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, Immunology, and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, CO 80523-1619</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name></author><author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name></author><author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name></author><author><name sortKey="Tesh, Robert B" sort="Tesh, Robert B" uniqKey="Tesh R" first="Robert B" last="Tesh">Robert B. Tesh</name></author><author><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Caco-2 Cells</term><term>Carboxypeptidases (genetics)</term><term>Carboxypeptidases (physiology)</term><term>Cats</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cricetinae</term><term>Herpestidae</term><term>Humans</term><term>Mice</term><term>Mink</term><term>Peptidyl-Dipeptidase A</term><term>Rabbits</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (physiology)</term><term>SARS Virus (growth & development)</term><term>SARS Virus (physiology)</term><term>Swine</term><term>Tupaia</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Carboxypeptidases (génétique)</term><term>Carboxypeptidases (physiologie)</term><term>Cellules Caco-2</term><term>Cellules Vero</term><term>Chats</term><term>Cricetinae</term><term>Herpestidae</term><term>Humains</term><term>Lapins</term><term>Lignée cellulaire</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (physiologie)</term><term>Réplication virale</term><term>Souris</term><term>Suidae</term><term>Tupaia</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (physiologie)</term><term>Visons</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carboxypeptidases</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Carboxypeptidases</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Carboxypeptidases</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Carboxypeptidases</term><term>Récepteurs viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Caco-2 Cells</term><term>Cats</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cricetinae</term><term>Herpestidae</term><term>Humans</term><term>Mice</term><term>Mink</term><term>Peptidyl-Dipeptidase A</term><term>Rabbits</term><term>Swine</term><term>Tupaia</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Caco-2</term><term>Cellules Vero</term><term>Chats</term><term>Cricetinae</term><term>Herpestidae</term><term>Humains</term><term>Lapins</term><term>Lignée cellulaire</term><term>Peptidyl-Dipeptidase A</term><term>Réplication virale</term><term>Souris</term><term>Suidae</term><term>Tupaia</term><term>Visons</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Of 30 cell lines and primary cells examined, productive severe acute respiratory syndrome coronavirus (Urbani strain) (SARS-CoV) infection after low-multiplicity inoculation was detected in only six: three African green monkey kidney epithelial cell lines (Vero, Vero E6, and MA104), a human colon epithelial line (CaCo-2), a porcine kidney epithelial line [PK(15)], and mink lung epithelial cells (Mv 1 Lu). SARS-CoV produced a lytic infection in Vero, Vero E6, and MA104 cells, but there was no visible cytopathic effect in Caco-2, Mv 1 Lu, or PK(15) cells. Multistep growth kinetics were identical in Vero E6 and MA104 cells, with maximum titer reached 24 h postinoculation (hpi). Virus titer was maximal 96 hpi in CaCo-2 cells, and virus was continually produced from infected CaCo-2 cells for at least 6 weeks after infection. CaCo-2 was the only human cell type of 13 tested that supported efficient SARS-CoV replication. Expression of the SARS-CoV receptor, angiotensin-converting enzyme 2 (ACE2), resulted in SARS-CoV replication in all refractory cell lines examined. Titers achieved were variable and dependent upon the method of ACE2 expression.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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