Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.
Identifieur interne : 002874 ( PubMed/Corpus ); précédent : 002873; suivant : 002875Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.
Auteurs : Eric C. Mossel ; Cheng Huang ; Krishna Narayanan ; Shinji Makino ; Robert B. Tesh ; C J PetersSource :
- Journal of virology [ 0022-538X ] ; 2005.
English descriptors
- KwdEn :
- Animals, Caco-2 Cells, Carboxypeptidases (genetics), Carboxypeptidases (physiology), Cats, Cell Line, Chlorocebus aethiops, Cricetinae, Herpestidae, Humans, Mice, Mink, Peptidyl-Dipeptidase A, Rabbits, Receptors, Virus (genetics), Receptors, Virus (physiology), SARS Virus (growth & development), SARS Virus (physiology), Swine, Tupaia, Vero Cells, Virus Replication.
- MESH :
- chemical , genetics : Carboxypeptidases, Receptors, Virus.
- chemical , physiology : Carboxypeptidases, Receptors, Virus.
- growth & development : SARS Virus.
- physiology : SARS Virus.
- Animals, Caco-2 Cells, Cats, Cell Line, Chlorocebus aethiops, Cricetinae, Herpestidae, Humans, Mice, Mink, Peptidyl-Dipeptidase A, Rabbits, Swine, Tupaia, Vero Cells, Virus Replication.
Abstract
Of 30 cell lines and primary cells examined, productive severe acute respiratory syndrome coronavirus (Urbani strain) (SARS-CoV) infection after low-multiplicity inoculation was detected in only six: three African green monkey kidney epithelial cell lines (Vero, Vero E6, and MA104), a human colon epithelial line (CaCo-2), a porcine kidney epithelial line [PK(15)], and mink lung epithelial cells (Mv 1 Lu). SARS-CoV produced a lytic infection in Vero, Vero E6, and MA104 cells, but there was no visible cytopathic effect in Caco-2, Mv 1 Lu, or PK(15) cells. Multistep growth kinetics were identical in Vero E6 and MA104 cells, with maximum titer reached 24 h postinoculation (hpi). Virus titer was maximal 96 hpi in CaCo-2 cells, and virus was continually produced from infected CaCo-2 cells for at least 6 weeks after infection. CaCo-2 was the only human cell type of 13 tested that supported efficient SARS-CoV replication. Expression of the SARS-CoV receptor, angiotensin-converting enzyme 2 (ACE2), resulted in SARS-CoV replication in all refractory cell lines examined. Titers achieved were variable and dependent upon the method of ACE2 expression.
DOI: 10.1128/JVI.79.6.3846-3850.2005
PubMed: 15731278
Links to Exploration step
pubmed:15731278Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.</title><author><name sortKey="Mossel, Eric C" sort="Mossel, Eric C" uniqKey="Mossel E" first="Eric C" last="Mossel">Eric C. Mossel</name><affiliation><nlm:affiliation>Department of Microbiology, Immunology, and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, CO 80523-1619, USA. ecmossel@colostate.edu</nlm:affiliation></affiliation></author><author><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name></author><author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name></author><author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name></author><author><name sortKey="Tesh, Robert B" sort="Tesh, Robert B" uniqKey="Tesh R" first="Robert B" last="Tesh">Robert B. Tesh</name></author><author><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15731278</idno><idno type="pmid">15731278</idno><idno type="doi">10.1128/JVI.79.6.3846-3850.2005</idno><idno type="wicri:Area/PubMed/Corpus">002874</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002874</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.</title><author><name sortKey="Mossel, Eric C" sort="Mossel, Eric C" uniqKey="Mossel E" first="Eric C" last="Mossel">Eric C. Mossel</name><affiliation><nlm:affiliation>Department of Microbiology, Immunology, and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, CO 80523-1619, USA. ecmossel@colostate.edu</nlm:affiliation></affiliation></author><author><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name></author><author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name></author><author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name></author><author><name sortKey="Tesh, Robert B" sort="Tesh, Robert B" uniqKey="Tesh R" first="Robert B" last="Tesh">Robert B. Tesh</name></author><author><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Caco-2 Cells</term><term>Carboxypeptidases (genetics)</term><term>Carboxypeptidases (physiology)</term><term>Cats</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cricetinae</term><term>Herpestidae</term><term>Humans</term><term>Mice</term><term>Mink</term><term>Peptidyl-Dipeptidase A</term><term>Rabbits</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (physiology)</term><term>SARS Virus (growth & development)</term><term>SARS Virus (physiology)</term><term>Swine</term><term>Tupaia</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carboxypeptidases</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Carboxypeptidases</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Caco-2 Cells</term><term>Cats</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cricetinae</term><term>Herpestidae</term><term>Humans</term><term>Mice</term><term>Mink</term><term>Peptidyl-Dipeptidase A</term><term>Rabbits</term><term>Swine</term><term>Tupaia</term><term>Vero Cells</term><term>Virus Replication</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Of 30 cell lines and primary cells examined, productive severe acute respiratory syndrome coronavirus (Urbani strain) (SARS-CoV) infection after low-multiplicity inoculation was detected in only six: three African green monkey kidney epithelial cell lines (Vero, Vero E6, and MA104), a human colon epithelial line (CaCo-2), a porcine kidney epithelial line [PK(15)], and mink lung epithelial cells (Mv 1 Lu). SARS-CoV produced a lytic infection in Vero, Vero E6, and MA104 cells, but there was no visible cytopathic effect in Caco-2, Mv 1 Lu, or PK(15) cells. Multistep growth kinetics were identical in Vero E6 and MA104 cells, with maximum titer reached 24 h postinoculation (hpi). Virus titer was maximal 96 hpi in CaCo-2 cells, and virus was continually produced from infected CaCo-2 cells for at least 6 weeks after infection. CaCo-2 was the only human cell type of 13 tested that supported efficient SARS-CoV replication. Expression of the SARS-CoV receptor, angiotensin-converting enzyme 2 (ACE2), resulted in SARS-CoV replication in all refractory cell lines examined. Titers achieved were variable and dependent upon the method of ACE2 expression.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15731278</PMID><DateCompleted><Year>2005</Year><Month>04</Month><Day>05</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-538X</ISSN><JournalIssue CitedMedium="Print"><Volume>79</Volume><Issue>6</Issue><PubDate><Year>2005</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.</ArticleTitle><Pagination><MedlinePgn>3846-50</MedlinePgn></Pagination><Abstract><AbstractText>Of 30 cell lines and primary cells examined, productive severe acute respiratory syndrome coronavirus (Urbani strain) (SARS-CoV) infection after low-multiplicity inoculation was detected in only six: three African green monkey kidney epithelial cell lines (Vero, Vero E6, and MA104), a human colon epithelial line (CaCo-2), a porcine kidney epithelial line [PK(15)], and mink lung epithelial cells (Mv 1 Lu). SARS-CoV produced a lytic infection in Vero, Vero E6, and MA104 cells, but there was no visible cytopathic effect in Caco-2, Mv 1 Lu, or PK(15) cells. Multistep growth kinetics were identical in Vero E6 and MA104 cells, with maximum titer reached 24 h postinoculation (hpi). Virus titer was maximal 96 hpi in CaCo-2 cells, and virus was continually produced from infected CaCo-2 cells for at least 6 weeks after infection. CaCo-2 was the only human cell type of 13 tested that supported efficient SARS-CoV replication. Expression of the SARS-CoV receptor, angiotensin-converting enzyme 2 (ACE2), resulted in SARS-CoV replication in all refractory cell lines examined. Titers achieved were variable and dependent upon the method of ACE2 expression.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mossel</LastName><ForeName>Eric C</ForeName><Initials>EC</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Immunology, and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, CO 80523-1619, USA. ecmossel@colostate.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Cheng</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Narayanan</LastName><ForeName>Krishna</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Makino</LastName><ForeName>Shinji</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Tesh</LastName><ForeName>Robert B</ForeName><Initials>RB</Initials></Author><Author ValidYN="Y"><LastName>Peters</LastName><ForeName>C J</ForeName><Initials>CJ</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>T32 AI007536</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>AI007536</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>AI29984</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21 AI029984</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 AI029984</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>N01 AI025489</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Virol</MedlineTA><NlmUniqueID>0113724</NlmUniqueID><ISSNLinking>0022-538X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011991">Receptors, Virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.-</RegistryNumber><NameOfSubstance UI="D002268">Carboxypeptidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.15.1</RegistryNumber><NameOfSubstance UI="D007703">Peptidyl-Dipeptidase A</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.17.-</RegistryNumber><NameOfSubstance UI="C413524">angiotensin converting enzyme 2</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" 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