SrasV1, Main, Merge, bibRecord, 004A99

Identification of Immunodominant Epitopes on the Membrane Protein of the Severe Acute Respiratory Syndrome-Associated Coronavirus

Identifieur interne : 004A99 ( Main/Merge ); précédent : 004A98; suivant : 004B00

Identification of Immunodominant Epitopes on the Membrane Protein of the Severe Acute Respiratory Syndrome-Associated Coronavirus

Auteurs : Yuxian He ; Yusen Zhou ; Pamela Siddiqui ; Jinkui Niu ; Shibo Jiang

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2005.

RBID : PMC:1234014

Descripteurs français

KwdFr :
- Animaux, Anticorps antiviraux (sang), Cartographie épitopique, Données de séquences moléculaires, Fragments peptidiques (immunologie), Humains, Immunisation, Lapins, Protéines de la matrice virale (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (diagnostic), Séquence d'acides aminés, Tests sérologiques, Virus du SRAS (immunologie), Épitopes immunodominants.
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Fragments peptidiques, Protéines de la matrice virale, Virus du SRAS.
- sang : Anticorps antiviraux.
- Animaux, Cartographie épitopique, Données de séquences moléculaires, Humains, Immunisation, Lapins, Souris, Souris de lignée BALB C, Séquence d'acides aminés, Tests sérologiques, Épitopes immunodominants.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Epitope Mapping, Humans, Immunization, Immunodominant Epitopes, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Peptide Fragments (immunology), Rabbits, SARS Virus (immunology), Serologic Tests, Severe Acute Respiratory Syndrome (diagnosis), Viral Matrix Proteins (immunology).
MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Peptide Fragments, Viral Matrix Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- Amino Acid Sequence, Animals, Epitope Mapping, Humans, Immunization, Immunodominant Epitopes, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Rabbits, Serologic Tests.

Abstract

Similar to other coronaviruses, the membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a major transmembrane glycoprotein with multiple biological functions. To date, limited information is available about its antigenic properties. In this study, we identified two major immunodominant epitopes on the M protein located in the extreme N-terminal region (residues 1 to 31) and the interior C-terminal region (residues 132 to 161), respectively, by Pepscan analyses against convalescent-phase sera from SARS patients and antisera from virus-immunized mice and rabbits. Synthetic peptides M1-31 derived from the N-terminal epitope and M132-161 derived from the C-terminal epitope were highly reactive with all of the convalescent-phase sera from 40 SARS patients but not with 30 control serum samples from healthy blood donors, suggesting their potential application for serologic diagnosis of SARS. We showed that both peptides (M1-31 and M132-161) were able to induce high titers of antibody responses in the immunized rabbits, highlighting their antigenicity and immunogenicity. These findings provide important information for developing SARS diagnostics and vaccines.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1234014

DOI: 10.1128/JCM.43.8.3718-3726.2005
PubMed: 16081901
PubMed Central: 1234014

Links to Exploration step

PMC:1234014

Le document en format XML

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<term>Humans</term>
<term>Immunization</term>
<term>Immunodominant Epitopes</term>
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<term>Mice, Inbred BALB C</term>
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<front><div type="abstract" xml:lang="en"><p>Similar to other coronaviruses, the membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a major transmembrane glycoprotein with multiple biological functions. To date, limited information is available about its antigenic properties. In this study, we identified two major immunodominant epitopes on the M protein located in the extreme N-terminal region (residues 1 to 31) and the interior C-terminal region (residues 132 to 161), respectively, by Pepscan analyses against convalescent-phase sera from SARS patients and antisera from virus-immunized mice and rabbits. Synthetic peptides M1-31 derived from the N-terminal epitope and M132-161 derived from the C-terminal epitope were highly reactive with all of the convalescent-phase sera from 40 SARS patients but not with 30 control serum samples from healthy blood donors, suggesting their potential application for serologic diagnosis of SARS. We showed that both peptides (M1-31 and M132-161) were able to induce high titers of antibody responses in the immunized rabbits, highlighting their antigenicity and immunogenicity. These findings provide important information for developing SARS diagnostics and vaccines.</p>
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Identification of Immunodominant Epitopes on the Membrane Protein of the Severe Acute Respiratory Syndrome-Associated Coronavirus

Identification of Immunodominant Epitopes on the Membrane Protein of the Severe Acute Respiratory Syndrome-Associated Coronavirus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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