Identification of immunodominant epitopes on the membrane protein of the severe acute respiratory syndrome-associated coronavirus.
Identifieur interne : 002605 ( PubMed/Corpus ); précédent : 002604; suivant : 002606Identification of immunodominant epitopes on the membrane protein of the severe acute respiratory syndrome-associated coronavirus.
Auteurs : Yuxian He ; Yusen Zhou ; Pamela Siddiqui ; Jinkui Niu ; Shibo JiangSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2005.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Epitope Mapping, Humans, Immunization, Immunodominant Epitopes, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Peptide Fragments (immunology), Rabbits, SARS Virus (immunology), Serologic Tests, Severe Acute Respiratory Syndrome (diagnosis), Viral Matrix Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Peptide Fragments, Viral Matrix Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- Amino Acid Sequence, Animals, Epitope Mapping, Humans, Immunization, Immunodominant Epitopes, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Rabbits, Serologic Tests.
Abstract
Similar to other coronaviruses, the membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a major transmembrane glycoprotein with multiple biological functions. To date, limited information is available about its antigenic properties. In this study, we identified two major immunodominant epitopes on the M protein located in the extreme N-terminal region (residues 1 to 31) and the interior C-terminal region (residues 132 to 161), respectively, by Pepscan analyses against convalescent-phase sera from SARS patients and antisera from virus-immunized mice and rabbits. Synthetic peptides M1-31 derived from the N-terminal epitope and M132-161 derived from the C-terminal epitope were highly reactive with all of the convalescent-phase sera from 40 SARS patients but not with 30 control serum samples from healthy blood donors, suggesting their potential application for serologic diagnosis of SARS. We showed that both peptides (M1-31 and M132-161) were able to induce high titers of antibody responses in the immunized rabbits, highlighting their antigenicity and immunogenicity. These findings provide important information for developing SARS diagnostics and vaccines.
DOI: 10.1128/JCM.43.8.3718-3726.2005
PubMed: 16081901
Links to Exploration step
pubmed:16081901Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of immunodominant epitopes on the membrane protein of the severe acute respiratory syndrome-associated coronavirus.</title><author><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name><affiliation><nlm:affiliation>Viral Immunology Laboratory, Lindsley F. Kimball Research Institute, New York Blood Center, 310 East 67th St., New York, NY 10021, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></author><author><name sortKey="Siddiqui, Pamela" sort="Siddiqui, Pamela" uniqKey="Siddiqui P" first="Pamela" last="Siddiqui">Pamela Siddiqui</name></author><author><name sortKey="Niu, Jinkui" sort="Niu, Jinkui" uniqKey="Niu J" first="Jinkui" last="Niu">Jinkui Niu</name></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16081901</idno><idno type="pmid">16081901</idno><idno type="doi">10.1128/JCM.43.8.3718-3726.2005</idno><idno type="wicri:Area/PubMed/Corpus">002605</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002605</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of immunodominant epitopes on the membrane protein of the severe acute respiratory syndrome-associated coronavirus.</title><author><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name><affiliation><nlm:affiliation>Viral Immunology Laboratory, Lindsley F. Kimball Research Institute, New York Blood Center, 310 East 67th St., New York, NY 10021, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></author><author><name sortKey="Siddiqui, Pamela" sort="Siddiqui, Pamela" uniqKey="Siddiqui P" first="Pamela" last="Siddiqui">Pamela Siddiqui</name></author><author><name sortKey="Niu, Jinkui" sort="Niu, Jinkui" uniqKey="Niu J" first="Jinkui" last="Niu">Jinkui Niu</name></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Epitope Mapping</term><term>Humans</term><term>Immunization</term><term>Immunodominant Epitopes</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Peptide Fragments (immunology)</term><term>Rabbits</term><term>SARS Virus (immunology)</term><term>Serologic Tests</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Viral Matrix Proteins (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Peptide Fragments</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Epitope Mapping</term><term>Humans</term><term>Immunization</term><term>Immunodominant Epitopes</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Rabbits</term><term>Serologic Tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Similar to other coronaviruses, the membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a major transmembrane glycoprotein with multiple biological functions. To date, limited information is available about its antigenic properties. In this study, we identified two major immunodominant epitopes on the M protein located in the extreme N-terminal region (residues 1 to 31) and the interior C-terminal region (residues 132 to 161), respectively, by Pepscan analyses against convalescent-phase sera from SARS patients and antisera from virus-immunized mice and rabbits. Synthetic peptides M1-31 derived from the N-terminal epitope and M132-161 derived from the C-terminal epitope were highly reactive with all of the convalescent-phase sera from 40 SARS patients but not with 30 control serum samples from healthy blood donors, suggesting their potential application for serologic diagnosis of SARS. We showed that both peptides (M1-31 and M132-161) were able to induce high titers of antibody responses in the immunized rabbits, highlighting their antigenicity and immunogenicity. These findings provide important information for developing SARS diagnostics and vaccines.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16081901</PMID><DateCompleted><Year>2005</Year><Month>09</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>43</Volume><Issue>8</Issue><PubDate><Year>2005</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Identification of immunodominant epitopes on the membrane protein of the severe acute respiratory syndrome-associated coronavirus.</ArticleTitle><Pagination><MedlinePgn>3718-26</MedlinePgn></Pagination><Abstract><AbstractText>Similar to other coronaviruses, the membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a major transmembrane glycoprotein with multiple biological functions. To date, limited information is available about its antigenic properties. In this study, we identified two major immunodominant epitopes on the M protein located in the extreme N-terminal region (residues 1 to 31) and the interior C-terminal region (residues 132 to 161), respectively, by Pepscan analyses against convalescent-phase sera from SARS patients and antisera from virus-immunized mice and rabbits. Synthetic peptides M1-31 derived from the N-terminal epitope and M132-161 derived from the C-terminal epitope were highly reactive with all of the convalescent-phase sera from 40 SARS patients but not with 30 control serum samples from healthy blood donors, suggesting their potential application for serologic diagnosis of SARS. We showed that both peptides (M1-31 and M132-161) were able to induce high titers of antibody responses in the immunized rabbits, highlighting their antigenicity and immunogenicity. These findings provide important information for developing SARS diagnostics and vaccines.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName><ForeName>Yuxian</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Viral Immunology Laboratory, Lindsley F. Kimball Research Institute, New York Blood Center, 310 East 67th St., New York, NY 10021, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yusen</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Siddiqui</LastName><ForeName>Pamela</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Niu</LastName><ForeName>Jinkui</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Shibo</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, 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