SrasV1, Main, Exploration, bibRecordById, pubmed:16014971

The severe acute respiratory syndrome coronavirus 3a protein up-regulates expression of fibrinogen in lung epithelial cells

Identifieur interne : 004D35 ( Main/Exploration ); précédent : 004D34; suivant : 004D36

The severe acute respiratory syndrome coronavirus 3a protein up-regulates expression of fibrinogen in lung epithelial cells

Auteurs : Yee-Joo Tan [Singapour] ; Puay-Yoke Tham [Singapour] ; Daphne Z. L. Chan [Singapour] ; Chih-Fong Chou [Singapour] ; SHUO SHEN [Singapour] ; Burtram C. Fielding [Singapour] ; Timothy H. P. Tan [Singapour] ; SENG GEE LIM [Singapour] ; WANJIN HONG [Singapour]

Source :

Journal of virology [ 0022-538X ] ; 2005.

RBID : Pascal:05-0333803

Descripteurs français

KwdFr :
- ARN messager (analyse), Animaux, Cellules Vero, Cellules épithéliales (métabolisme), Cellules épithéliales (virologie), Fibrinogène (génétique), Fibrinogène (métabolisme), Humains, Lignée cellulaire, Protéines virales (métabolisme), Réaction de polymérisation en chaîne, Régulation positive, Technique de Western, Virus du SRAS.
MESH :
- analyse : ARN messager.
- génétique : Fibrinogène.
- métabolisme : Cellules épithéliales, Fibrinogène, Protéines virales.
- virologie : Cellules épithéliales.
Pascal (Inist)
- Animaux, Cellules Vero, Coronavirus, Humains, Lignée cellulaire, Protéine, Réaction de polymérisation en chaîne, Régulation, Fibrinogène, Cellule épithéliale, Microbiologie, Régulation positive, Technique de Western, Virologie, Syndrome respiratoire aigu sévère, Virus du SRAS.

English descriptors

KwdEn :
- Animals, Blotting, Western, Cell Line, Chlorocebus aethiops, Coronavirus, Epithelial Cells (metabolism), Epithelial Cells (virology), Epithelial cell, Fibrinogen, Fibrinogen (genetics), Fibrinogen (metabolism), Humans, Microbiology, Polymerase Chain Reaction, Protein, RNA, Messenger (analysis), Regulation(control), SARS Virus, Severe acute respiratory syndrome, Up-Regulation, Vero Cells, Viral Proteins (metabolism), Virology.
MESH :
- chemical , analysis : RNA, Messenger.
- chemical , genetics : Fibrinogen.
- metabolism : Epithelial Cells, Fibrinogen, Viral Proteins.
- virology : Epithelial Cells.
- Animals, Blotting, Western, Cell Line, Chlorocebus aethiops, Humans, Polymerase Chain Reaction, SARS Virus, Up-Regulation, Vero Cells.

Abstract

Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aa, Bβ, and γ, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1181587

Affiliations:

Singapour

Le document en format XML

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<term>Cell Line</term>
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<term>Coronavirus</term>
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<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Blotting, Western</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Polymerase Chain Reaction</term>
<term>SARS Virus</term>
<term>Up-Regulation</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Coronavirus</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Protéine</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Régulation</term>
<term>Fibrinogène</term>
<term>Cellule épithéliale</term>
<term>Microbiologie</term>
<term>Régulation positive</term>
<term>Technique de Western</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aa, Bβ, and γ, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.</div>
</front>
</TEI>
<affiliations><list><country><li>Singapour</li>
</country>
</list>
<tree><country name="Singapour"><noRegion><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name>
</noRegion>
<name sortKey="Chan, Daphne Z L" sort="Chan, Daphne Z L" uniqKey="Chan D" first="Daphne Z. L." last="Chan">Daphne Z. L. Chan</name>
<name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name>
<name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name>
<name sortKey="Seng Gee Lim" sort="Seng Gee Lim" uniqKey="Seng Gee Lim" last="Seng Gee Lim">SENG GEE LIM</name>
<name sortKey="Shuo Shen" sort="Shuo Shen" uniqKey="Shuo Shen" last="Shuo Shen">SHUO SHEN</name>
<name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name>
<name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name>
<name sortKey="Wanjin Hong" sort="Wanjin Hong" uniqKey="Wanjin Hong" last="Wanjin Hong">WANJIN HONG</name>
</country>
</tree>
</affiliations>
</record>

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The severe acute respiratory syndrome coronavirus 3a protein up-regulates expression of fibrinogen in lung epithelial cells

The severe acute respiratory syndrome coronavirus 3a protein up-regulates expression of fibrinogen in lung epithelial cells

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