The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells
Identifieur interne : 000695 ( Pmc/Corpus ); précédent : 000694; suivant : 000696The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells
Auteurs : Yee-Joo Tan ; Puay-Yoke Tham ; Daphne Z. L. Chan ; Chih-Fong Chou ; Shuo Shen ; Burtram C. Fielding ; Timothy H. P. Tan ; Seng Gee Lim ; Wanjin HongSource :
- Journal of Virology [ 0022-538X ] ; 2005.
Abstract
Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aα, Bβ, and γ, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.
Url:
DOI: 10.1128/JVI.79.15.10083-10087.2005
PubMed: 16014971
PubMed Central: 1181587
Links to Exploration step
PMC:1181587Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells</title><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Chan, Daphne Z L" sort="Chan, Daphne Z L" uniqKey="Chan D" first="Daphne Z. L." last="Chan">Daphne Z. L. Chan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16014971</idno><idno type="pmc">1181587</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1181587</idno><idno type="RBID">PMC:1181587</idno><idno type="doi">10.1128/JVI.79.15.10083-10087.2005</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000695</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000695</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells</title><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Chan, Daphne Z L" sort="Chan, Daphne Z L" uniqKey="Chan D" first="Daphne Z. L." last="Chan">Daphne Z. L. Chan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aα, Bβ, and γ, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id><journal-id journal-id-type="publisher-id">jvi</journal-id><journal-title>Journal of Virology</journal-title><issn pub-type="ppub">0022-538X</issn><issn pub-type="epub">1098-5514</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16014971</article-id><article-id pub-id-type="pmc">1181587</article-id><article-id pub-id-type="publisher-id">0571-05</article-id><article-id pub-id-type="doi">10.1128/JVI.79.15.10083-10087.2005</article-id><article-categories><subj-group subj-group-type="heading"><subject>Cellular Response to Infection</subject></subj-group></article-categories><title-group><article-title>The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Tan</surname><given-names>Yee-Joo</given-names></name><xref ref-type="aff" rid="aff0"></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Tham</surname><given-names>Puay-Yoke</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Daphne Z. L.</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Chou</surname><given-names>Chih-Fong</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Shen</surname><given-names>Shuo</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Fielding</surname><given-names>Burtram C.</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Tan</surname><given-names>Timothy H. P.</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Lim</surname><given-names>Seng Gee</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Hong</surname><given-names>Wanjin</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib></contrib-group><aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673. Phone: 65-65869625. Fax: 65-67791117. E-mail: <email>mcbtanyj@imcb.a-star.edu.sg</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>8</month><year>2005</year></pub-date><volume>79</volume><issue>15</issue><fpage>10083</fpage><lpage>10087</lpage><history><date date-type="received"><day>20</day><month>3</month><year>2005</year></date><date date-type="accepted"><day>18</day><month>4</month><year>2005</year></date></history><copyright-statement>Copyright © 2005, American Society for Microbiology</copyright-statement><copyright-year>2005</copyright-year><abstract><p>Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aα, Bβ, and γ, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.</p></abstract><custom-meta-wrap><custom-meta><meta-name>PDF filename</meta-name><meta-value>zjv01505010083</meta-value></custom-meta></custom-meta-wrap></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000695 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000695 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé= PMC:1181587
|texte= The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:16014971" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |