The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells
Identifieur interne : 001057 ( Ncbi/Merge ); précédent : 001056; suivant : 001058The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells
Auteurs : Yee-Joo Tan ; Puay-Yoke Tham ; Daphne Z. L. Chan ; Chih-Fong Chou ; Shuo Shen ; Burtram C. Fielding ; Timothy H. P. Tan ; Seng Gee Lim ; Wanjin HongSource :
- Journal of Virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- ARN messager (analyse), Animaux, Cellules Vero, Cellules épithéliales (métabolisme), Cellules épithéliales (virologie), Fibrinogène (génétique), Fibrinogène (métabolisme), Humains, Lignée cellulaire, Protéines virales (métabolisme), Réaction de polymérisation en chaîne, Régulation positive, Technique de Western, Virus du SRAS.
- MESH :
- analyse : ARN messager.
- génétique : Fibrinogène.
- métabolisme : Cellules épithéliales, Fibrinogène, Protéines virales.
- virologie : Cellules épithéliales.
- Animaux, Cellules Vero, Humains, Lignée cellulaire, Réaction de polymérisation en chaîne, Régulation positive, Technique de Western, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Messenger.
- chemical , genetics : Fibrinogen.
- metabolism : Epithelial Cells, Fibrinogen, Viral Proteins.
- virology : Epithelial Cells.
- Animals, Blotting, Western, Cell Line, Chlorocebus aethiops, Humans, Polymerase Chain Reaction, SARS Virus, Up-Regulation, Vero Cells.
Abstract
Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aα, Bβ, and γ, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.
Url:
DOI: 10.1128/JVI.79.15.10083-10087.2005
PubMed: 16014971
PubMed Central: 1181587
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PMC:1181587Le document en format XML
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L." last="Chan">Daphne Z. L. Chan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Epithelial Cells (metabolism)</term><term>Epithelial Cells (virology)</term><term>Fibrinogen (genetics)</term><term>Fibrinogen (metabolism)</term><term>Humans</term><term>Polymerase Chain Reaction</term><term>RNA, Messenger (analysis)</term><term>SARS Virus</term><term>Up-Regulation</term><term>Vero Cells</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (analyse)</term><term>Animaux</term><term>Cellules Vero</term><term>Cellules épithéliales (métabolisme)</term><term>Cellules épithéliales (virologie)</term><term>Fibrinogène (génétique)</term><term>Fibrinogène (métabolisme)</term><term>Humains</term><term>Lignée cellulaire</term><term>Protéines virales (métabolisme)</term><term>Réaction de polymérisation en chaîne</term><term>Régulation positive</term><term>Technique de Western</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fibrinogen</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fibrinogène</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term><term>Fibrinogen</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules épithéliales</term><term>Fibrinogène</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Polymerase Chain Reaction</term><term>SARS Virus</term><term>Up-Regulation</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Humains</term><term>Lignée cellulaire</term><term>Réaction de polymérisation en chaîne</term><term>Régulation positive</term><term>Technique de Western</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aα, Bβ, and γ, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.</p></div></front></TEI><double pmid="16014971"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells</title><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Chan, Daphne Z L" sort="Chan, Daphne Z L" uniqKey="Chan D" first="Daphne Z. L." last="Chan">Daphne Z. L. Chan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16014971</idno><idno type="pmc">1181587</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1181587</idno><idno type="RBID">PMC:1181587</idno><idno type="doi">10.1128/JVI.79.15.10083-10087.2005</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000695</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000695</idno><idno type="wicri:Area/Pmc/Curation">000695</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000695</idno><idno type="wicri:Area/Pmc/Checkpoint">001209</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001209</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Severe Acute Respiratory Syndrome Coronavirus 3a Protein Up-Regulates Expression of Fibrinogen in Lung Epithelial Cells</title><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Chan, Daphne Z L" sort="Chan, Daphne Z L" uniqKey="Chan D" first="Daphne Z. L." last="Chan">Daphne Z. L. Chan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><affiliation><nlm:aff id="aff0">Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673</nlm:aff><wicri:noCountry code="subfield">Singapore 138673</wicri:noCountry></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aα, Bβ, and γ, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.</p></div></front></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The severe acute respiratory syndrome coronavirus 3a protein up-regulates expression of fibrinogen in lung epithelial cells.</title><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Proteos, Singapore. mcbtanyj@imcb.a-star.edu.sg</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Institute of Molecular and Cell Biology, Proteos</wicri:regionArea><wicri:noRegion>Proteos</wicri:noRegion></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name></author><author><name sortKey="Chan, Daphne Z L" sort="Chan, Daphne Z L" uniqKey="Chan D" first="Daphne Z L" last="Chan">Daphne Z L. Chan</name></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name></author><author><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C" last="Fielding">Burtram C. Fielding</name></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H P" last="Tan">Timothy H P. Tan</name></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16014971</idno><idno type="pmid">16014971</idno><idno type="doi">10.1128/JVI.79.15.10083-10087.2005</idno><idno type="wicri:Area/PubMed/Corpus">002643</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002643</idno><idno type="wicri:Area/PubMed/Curation">002643</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002643</idno><idno type="wicri:Area/PubMed/Checkpoint">002417</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002417</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The severe acute respiratory syndrome coronavirus 3a protein up-regulates expression of fibrinogen in lung epithelial cells.</title><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Proteos, Singapore. mcbtanyj@imcb.a-star.edu.sg</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Institute of Molecular and Cell Biology, Proteos</wicri:regionArea><wicri:noRegion>Proteos</wicri:noRegion></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name></author><author><name sortKey="Chan, Daphne Z L" sort="Chan, Daphne Z L" uniqKey="Chan D" first="Daphne Z L" last="Chan">Daphne Z L. Chan</name></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name></author><author><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C" last="Fielding">Burtram C. Fielding</name></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H P" last="Tan">Timothy H P. Tan</name></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Epithelial Cells (metabolism)</term><term>Epithelial Cells (virology)</term><term>Fibrinogen (genetics)</term><term>Fibrinogen (metabolism)</term><term>Humans</term><term>Polymerase Chain Reaction</term><term>RNA, Messenger (analysis)</term><term>SARS Virus</term><term>Up-Regulation</term><term>Vero Cells</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (analyse)</term><term>Animaux</term><term>Cellules Vero</term><term>Cellules épithéliales (métabolisme)</term><term>Cellules épithéliales (virologie)</term><term>Fibrinogène (génétique)</term><term>Fibrinogène (métabolisme)</term><term>Humains</term><term>Lignée cellulaire</term><term>Protéines virales (métabolisme)</term><term>Réaction de polymérisation en chaîne</term><term>Régulation positive</term><term>Technique de Western</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fibrinogen</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fibrinogène</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term><term>Fibrinogen</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules épithéliales</term><term>Fibrinogène</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Polymerase Chain Reaction</term><term>SARS Virus</term><term>Up-Regulation</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Humains</term><term>Lignée cellulaire</term><term>Réaction de polymérisation en chaîne</term><term>Régulation positive</term><term>Technique de Western</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aalpha, Bbeta, and gamma, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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