Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay
Identifieur interne : 001C80 ( Main/Exploration ); précédent : 001C79; suivant : 001C81Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay
Auteurs : Hoon H. Sunwoo ; Arivazhagan Palaniyappan ; Advaita Ganguly ; Pravin K. Bhatnagar ; Dipankar Das ; Ayman O. S. El-Kadi ; Mavanur R. SureshSource :
- Journal of Virological Methods [ 0166-0934 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps bispécifiques (immunologie), Anticorps monoclonaux (immunologie), Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (analyse), Glycoprotéines membranaires (immunologie), Humains, Lignée cellulaire, Protéines de l'enveloppe virale (analyse), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (analyse), Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Sensibilité et spécificité, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Test ELISA, Vaccins antiviraux (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- analyse : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside.
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Virus du SRAS.
- immunologie : Anticorps antiviraux, Anticorps bispécifiques, Anticorps monoclonaux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines recombinantes, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Sensibilité et spécificité, Souris, Souris de lignée BALB C, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Bispecific (immunology), Antibodies, Monoclonal (immunology), Antibodies, Viral (immunology), Cell Line, Enzyme-Linked Immunosorbent Assay, Female, Humans, Membrane Glycoproteins (analysis), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (analysis), Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , analysis : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Bispecific, Antibodies, Monoclonal, Antibodies, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Recombinant Proteins, Viral Envelope Proteins, Viral Vaccines.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Enzyme-Linked Immunosorbent Assay, Female, Humans, Mice, Mice, Inbred BALB C, Sensitivity and Specificity, Spike Glycoprotein, Coronavirus.
Abstract
► Two monoclonal antibodies against SARS CoV S1 antigen were generated by hybridomatechnology. ► Bispecific antibody (bsmAb) was developed by Quadroma technology. ► Mono- and bispecific antibodies were evaluated by sandiwichELISA for immunodiagnosis. ► We report that bsmAb-based ELISA is more sensitive than the mAb-based ELISA for detection of the S1 antigen.
Url:
DOI: 10.1016/j.jviromet.2012.09.006
PubMed: 22995576
PubMed Central: 7112864
Affiliations:
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Le document en format XML
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<term>Enzyme-Linked Immunosorbent Assay</term>
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<term>Humans</term>
<term>Membrane Glycoproteins (analysis)</term>
<term>Membrane Glycoproteins (immunology)</term>
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<term>Mice, Inbred BALB C</term>
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<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (analyse)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
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<term>Lignée cellulaire</term>
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<term>Protéines de l'enveloppe virale (immunologie)</term>
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<term>Protéines nucléocapside (immunologie)</term>
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<term>Recombinant Proteins</term>
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<term>Anticorps bispécifiques</term>
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<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines nucléocapside</term>
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<front><div type="abstract" xml:lang="en"><title>Highlights</title>
<p>► Two monoclonal antibodies against SARS CoV S1 antigen were generated by hybridomatechnology. ► Bispecific antibody (bsmAb) was developed by Quadroma technology. ► Mono- and bispecific antibodies were evaluated by sandiwichELISA for immunodiagnosis. ► We report that bsmAb-based ELISA is more sensitive than the mAb-based ELISA for detection of the S1 antigen.</p>
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</back>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Bhatnagar, Pravin K" sort="Bhatnagar, Pravin K" uniqKey="Bhatnagar P" first="Pravin K." last="Bhatnagar">Pravin K. Bhatnagar</name>
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<name sortKey="El Kadi, Ayman O S" sort="El Kadi, Ayman O S" uniqKey="El Kadi A" first="Ayman O. S." last="El-Kadi">Ayman O. S. El-Kadi</name>
<name sortKey="Ganguly, Advaita" sort="Ganguly, Advaita" uniqKey="Ganguly A" first="Advaita" last="Ganguly">Advaita Ganguly</name>
<name sortKey="Palaniyappan, Arivazhagan" sort="Palaniyappan, Arivazhagan" uniqKey="Palaniyappan A" first="Arivazhagan" last="Palaniyappan">Arivazhagan Palaniyappan</name>
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</affiliations>
</record>
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