Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay.
Identifieur interne : 001302 ( PubMed/Corpus ); précédent : 001301; suivant : 001303Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay.
Auteurs : Hoon H. Sunwoo ; Arivazhagan Palaniyappan ; Advaita Ganguly ; Pravin K. Bhatnagar ; Dipankar Das ; Ayman O S. El-Kadi ; Mavanur R. SureshSource :
- Journal of virological methods [ 1879-0984 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Antibodies, Bispecific (immunology), Antibodies, Monoclonal (immunology), Antibodies, Viral (immunology), Cell Line, Enzyme-Linked Immunosorbent Assay, Female, Humans, Membrane Glycoproteins (analysis), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (analysis), Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , analysis : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Bispecific, Antibodies, Monoclonal, Antibodies, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Recombinant Proteins, Viral Envelope Proteins, Viral Vaccines.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Enzyme-Linked Immunosorbent Assay, Female, Humans, Mice, Mice, Inbred BALB C, Sensitivity and Specificity, Spike Glycoprotein, Coronavirus.
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein is known to mediate receptor interaction and immune recognition and thus it is considered as a major target for vaccine design. The spike protein plays an important role in virus entry, virus receptor interactions, and virus tropism. Sensitive diagnosis of SARS is essential for the control of the disease in humans. Recombinant SARS-CoV S1 antigen was produced and purified for the development of monoclonal and bi-specific monoclonal antibodies. The hybridomas secreting anti-S1 antibodies, F26G18 and P136.8D12, were fused respectively with the YP4 hybridoma to generate quadromas. The sandwich ELISA was formed by using F26G18 as a coating antibody and biotinylated F26G18 as a detection antibody with a detection limit of 0.037μg/ml (p<0.02). The same detection limit was found with P136.8D12 as a coating antibody and biotinylated F26G18 as a detection antibody. The sensitivity was improved (detection limit of 0.019μg/ml), however, when using bi-specific monoclonal antibody (F157) as the detection antibody. In conclusion, the method described in this study allows sensitive detection of a recombinant SARS spike protein by sandwich ELISA with bi-specific monoclonal antibody and could be used for the diagnosis of patients suspected with SARS.
DOI: 10.1016/j.jviromet.2012.09.006
PubMed: 22995576
Links to Exploration step
pubmed:22995576Le document en format XML
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El-Kadi</name></author><author><name sortKey="Suresh, Mavanur R" sort="Suresh, Mavanur R" uniqKey="Suresh M" first="Mavanur R" last="Suresh">Mavanur R. Suresh</name></author></analytic><series><title level="j">Journal of virological methods</title><idno type="eISSN">1879-0984</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Bispecific (immunology)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Cell Line</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Humans</term><term>Membrane Glycoproteins (analysis)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Nucleocapsid Proteins (analysis)</term><term>Nucleocapsid Proteins (immunology)</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (analysis)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Bispecific</term><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Sensitivity and Specificity</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein is known to mediate receptor interaction and immune recognition and thus it is considered as a major target for vaccine design. The spike protein plays an important role in virus entry, virus receptor interactions, and virus tropism. Sensitive diagnosis of SARS is essential for the control of the disease in humans. Recombinant SARS-CoV S1 antigen was produced and purified for the development of monoclonal and bi-specific monoclonal antibodies. The hybridomas secreting anti-S1 antibodies, F26G18 and P136.8D12, were fused respectively with the YP4 hybridoma to generate quadromas. The sandwich ELISA was formed by using F26G18 as a coating antibody and biotinylated F26G18 as a detection antibody with a detection limit of 0.037μg/ml (p<0.02). The same detection limit was found with P136.8D12 as a coating antibody and biotinylated F26G18 as a detection antibody. The sensitivity was improved (detection limit of 0.019μg/ml), however, when using bi-specific monoclonal antibody (F157) as the detection antibody. In conclusion, the method described in this study allows sensitive detection of a recombinant SARS spike protein by sandwich ELISA with bi-specific monoclonal antibody and could be used for the diagnosis of patients suspected with SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22995576</PMID><DateCompleted><Year>2013</Year><Month>06</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0984</ISSN><JournalIssue CitedMedium="Internet"><Volume>187</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of virological methods</Title><ISOAbbreviation>J. Virol. Methods</ISOAbbreviation></Journal><ArticleTitle>Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay.</ArticleTitle><Pagination><MedlinePgn>72-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jviromet.2012.09.006</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0166-0934(12)00317-5</ELocationID><Abstract><AbstractText>The severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein is known to mediate receptor interaction and immune recognition and thus it is considered as a major target for vaccine design. The spike protein plays an important role in virus entry, virus receptor interactions, and virus tropism. Sensitive diagnosis of SARS is essential for the control of the disease in humans. Recombinant SARS-CoV S1 antigen was produced and purified for the development of monoclonal and bi-specific monoclonal antibodies. The hybridomas secreting anti-S1 antibodies, F26G18 and P136.8D12, were fused respectively with the YP4 hybridoma to generate quadromas. The sandwich ELISA was formed by using F26G18 as a coating antibody and biotinylated F26G18 as a detection antibody with a detection limit of 0.037μg/ml (p<0.02). The same detection limit was found with P136.8D12 as a coating antibody and biotinylated F26G18 as a detection antibody. The sensitivity was improved (detection limit of 0.019μg/ml), however, when using bi-specific monoclonal antibody (F157) as the detection antibody. In conclusion, the method described in this study allows sensitive detection of a recombinant SARS spike protein by sandwich ELISA with bi-specific monoclonal antibody and could be used for the diagnosis of patients suspected with SARS.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sunwoo</LastName><ForeName>Hoon H</ForeName><Initials>HH</Initials><AffiliationInfo><Affiliation>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11361-87 Avenue, Edmonton, Alberta, Canada. hsunwoo@ualberta.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Palaniyappan</LastName><ForeName>Arivazhagan</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Ganguly</LastName><ForeName>Advaita</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bhatnagar</LastName><ForeName>Pravin K</ForeName><Initials>PK</Initials></Author><Author ValidYN="Y"><LastName>Das</LastName><ForeName>Dipankar</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>El-Kadi</LastName><ForeName>Ayman O S</ForeName><Initials>AO</Initials></Author><Author ValidYN="Y"><LastName>Suresh</LastName><ForeName>Mavanur R</ForeName><Initials>MR</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>U01 AI061233</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>5U01AI061233-05</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Virol Methods</MedlineTA><NlmUniqueID>8005839</NlmUniqueID><ISSNLinking>0166-0934</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018033">Antibodies, 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PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22995576</ArticleId><ArticleId IdType="pii">S0166-0934(12)00317-5</ArticleId><ArticleId IdType="doi">10.1016/j.jviromet.2012.09.006</ArticleId><ArticleId IdType="pmc">PMC7112864</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proteomics. 2004 Feb;4(2):492-504</Citation><ArticleIdList><ArticleId IdType="pubmed">14760722</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2001 Jan 20;279(2):371-4</Citation><ArticleIdList><ArticleId IdType="pubmed">11162792</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Recognit. 2008 Mar-Apr;21(2):122-31</Citation><ArticleIdList><ArticleId IdType="pubmed">18383098</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2003 Dec 26;312(4):1159-64</Citation><ArticleIdList><ArticleId IdType="pubmed">14651994</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2003 Oct 10;310(1):78-83</Citation><ArticleIdList><ArticleId IdType="pubmed">14511651</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Infect Dis. 2004 Jan 15;38(2):297-9</Citation><ArticleIdList><ArticleId IdType="pubmed">14699466</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Methods Mol Biol. 2011;665:369-82</Citation><ArticleIdList><ArticleId IdType="pubmed">21116811</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Microbiol. 2004 Apr;42(4):1570-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15071006</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2008 Nov;82(22):11318-30</Citation><ArticleIdList><ArticleId IdType="pubmed">18753196</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 Jul 31;349(5):508-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12890855</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4</Citation><ArticleIdList><ArticleId IdType="pubmed">388439</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2003 Aug;77(16):8801-11</Citation><ArticleIdList><ArticleId IdType="pubmed">12885899</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Virol. 2003 Dec;28(3):233-8</Citation><ArticleIdList><ArticleId IdType="pubmed">14522060</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>MAbs. 2010 Jan-Feb;2(1):53-66</Citation><ArticleIdList><ArticleId IdType="pubmed">20168090</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 1975 Aug 7;256(5517):495-7</Citation><ArticleIdList><ArticleId IdType="pubmed">1172191</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1948-51</Citation><ArticleIdList><ArticleId IdType="pubmed">12748314</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Jan 23;313(4):938-47</Citation><ArticleIdList><ArticleId IdType="pubmed">14706633</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Microbiol. 2005 Jul;43(7):3054-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16000415</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation><ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2004 May 14;279(20):20836-49</Citation><ArticleIdList><ArticleId IdType="pubmed">14996844</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochemistry. 2005 Jan 25;44(3):947-58</Citation><ArticleIdList><ArticleId IdType="pubmed">15654751</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Jul;78(13):7217-26</Citation><ArticleIdList><ArticleId IdType="pubmed">15194798</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>New Microbiol. 2012 Jan;35(1):1-16</Citation><ArticleIdList><ArticleId IdType="pubmed">22378548</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunology. 2007 Dec;122(4):496-502</Citation><ArticleIdList><ArticleId IdType="pubmed">17680799</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2006 Jun;80(12):5757-67</Citation><ArticleIdList><ArticleId IdType="pubmed">16731915</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Poult Sci. 2012 Mar;91(3):636-42</Citation><ArticleIdList><ArticleId IdType="pubmed">22334738</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol Methods. 2008 Sep;152(1-2):77-84</Citation><ArticleIdList><ArticleId IdType="pubmed">18620761</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Genet Evol. 2009 Dec;9(6):1185-96</Citation><ArticleIdList><ArticleId IdType="pubmed">19800030</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol. 2008 Oct 15;181(8):5490-500</Citation><ArticleIdList><ArticleId IdType="pubmed">18832706</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Diagn Lab Immunol. 2004 Mar;11(2):362-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15013989</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol Methods. 2005 Jul;127(1):46-53</Citation><ArticleIdList><ArticleId IdType="pubmed">15893565</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2006 Feb 10;281(6):3198-203</Citation><ArticleIdList><ArticleId IdType="pubmed">16339146</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Vaccine Immunol. 2009 Feb;16(2):241-5</Citation><ArticleIdList><ArticleId IdType="pubmed">19038782</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14040-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16169905</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2005 Sep 16;309(5742):1864-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16166518</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2010 Apr;91(Pt 4):1058-62</Citation><ArticleIdList><ArticleId IdType="pubmed">20016037</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Diagn Lab Immunol. 2004 Jul;11(4):752-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15242951</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Vaccine Immunol. 2007 Feb;14(2):146-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17202310</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Jul 9;319(4):1216-21</Citation><ArticleIdList><ArticleId IdType="pubmed">15194496</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Rev Mol Cell Biol. 2003 Apr;4(4):309-19</Citation><ArticleIdList><ArticleId IdType="pubmed">12671653</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol J. 2010 May 17;7:99</Citation><ArticleIdList><ArticleId IdType="pubmed">20478047</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Cell Proteomics. 2003 May;2(5):346-56</Citation><ArticleIdList><ArticleId IdType="pubmed">12775768</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Comp Med. 2007 Oct;57(5):450-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17974127</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2536-41</Citation><ArticleIdList><ArticleId IdType="pubmed">14983044</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 May;78(9):4552-60</Citation><ArticleIdList><ArticleId 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