Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay
Identifieur interne : 000A40 ( Pmc/Checkpoint ); précédent : 000A39; suivant : 000A41Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay
Auteurs : Hoon H. Sunwoo ; Arivazhagan Palaniyappan ; Advaita Ganguly ; Pravin K. Bhatnagar ; Dipankar Das ; Ayman O. S. El-Kadi ; Mavanur R. SureshSource :
- Journal of Virological Methods [ 0166-0934 ] ; 2012.
Abstract
► Two monoclonal antibodies against SARS CoV S1 antigen were generated by hybridomatechnology. ► Bispecific antibody (bsmAb) was developed by Quadroma technology. ► Mono- and bispecific antibodies were evaluated by sandiwichELISA for immunodiagnosis. ► We report that bsmAb-based ELISA is more sensitive than the mAb-based ELISA for detection of the S1 antigen.
Url:
DOI: 10.1016/j.jviromet.2012.09.006
PubMed: 22995576
PubMed Central: 7112864
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:7112864Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay</title><author><name sortKey="Sunwoo, Hoon H" sort="Sunwoo, Hoon H" uniqKey="Sunwoo H" first="Hoon H." last="Sunwoo">Hoon H. Sunwoo</name></author><author><name sortKey="Palaniyappan, Arivazhagan" sort="Palaniyappan, Arivazhagan" uniqKey="Palaniyappan A" first="Arivazhagan" last="Palaniyappan">Arivazhagan Palaniyappan</name></author><author><name sortKey="Ganguly, Advaita" sort="Ganguly, Advaita" uniqKey="Ganguly A" first="Advaita" last="Ganguly">Advaita Ganguly</name></author><author><name sortKey="Bhatnagar, Pravin K" sort="Bhatnagar, Pravin K" uniqKey="Bhatnagar P" first="Pravin K." last="Bhatnagar">Pravin K. Bhatnagar</name></author><author><name sortKey="Das, Dipankar" sort="Das, Dipankar" uniqKey="Das D" first="Dipankar" last="Das">Dipankar Das</name></author><author><name sortKey="El Kadi, Ayman O S" sort="El Kadi, Ayman O S" uniqKey="El Kadi A" first="Ayman O. S." last="El-Kadi">Ayman O. S. El-Kadi</name></author><author><name sortKey="Suresh, Mavanur R" sort="Suresh, Mavanur R" uniqKey="Suresh M" first="Mavanur R." last="Suresh">Mavanur R. Suresh</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22995576</idno><idno type="pmc">7112864</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112864</idno><idno type="RBID">PMC:7112864</idno><idno type="doi">10.1016/j.jviromet.2012.09.006</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">001234</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001234</idno><idno type="wicri:Area/Pmc/Curation">001234</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001234</idno><idno type="wicri:Area/Pmc/Checkpoint">000A40</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A40</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay</title><author><name sortKey="Sunwoo, Hoon H" sort="Sunwoo, Hoon H" uniqKey="Sunwoo H" first="Hoon H." last="Sunwoo">Hoon H. Sunwoo</name></author><author><name sortKey="Palaniyappan, Arivazhagan" sort="Palaniyappan, Arivazhagan" uniqKey="Palaniyappan A" first="Arivazhagan" last="Palaniyappan">Arivazhagan Palaniyappan</name></author><author><name sortKey="Ganguly, Advaita" sort="Ganguly, Advaita" uniqKey="Ganguly A" first="Advaita" last="Ganguly">Advaita Ganguly</name></author><author><name sortKey="Bhatnagar, Pravin K" sort="Bhatnagar, Pravin K" uniqKey="Bhatnagar P" first="Pravin K." last="Bhatnagar">Pravin K. Bhatnagar</name></author><author><name sortKey="Das, Dipankar" sort="Das, Dipankar" uniqKey="Das D" first="Dipankar" last="Das">Dipankar Das</name></author><author><name sortKey="El Kadi, Ayman O S" sort="El Kadi, Ayman O S" uniqKey="El Kadi A" first="Ayman O. S." last="El-Kadi">Ayman O. S. El-Kadi</name></author><author><name sortKey="Suresh, Mavanur R" sort="Suresh, Mavanur R" uniqKey="Suresh M" first="Mavanur R." last="Suresh">Mavanur R. Suresh</name></author></analytic><series><title level="j">Journal of Virological Methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p>► Two monoclonal antibodies against SARS CoV S1 antigen were generated by hybridomatechnology. ► Bispecific antibody (bsmAb) was developed by Quadroma technology. ► Mono- and bispecific antibodies were evaluated by sandiwichELISA for immunodiagnosis. ► We report that bsmAb-based ELISA is more sensitive than the mAb-based ELISA for detection of the S1 antigen.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Babcock, G J" uniqKey="Babcock G">G.J. Babcock</name></author><author><name sortKey="Esshaki, D J" uniqKey="Esshaki D">D.J. Esshaki</name></author><author><name sortKey="Thomas, W D" uniqKey="Thomas W">W.D. Thomas</name></author><author><name sortKey="Ambrosino, D M" uniqKey="Ambrosino D">D.M. Ambrosino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balboni, A" uniqKey="Balboni A">A. Balboni</name></author><author><name sortKey="Battilani, M" uniqKey="Battilani M">M. Battilani</name></author><author><name sortKey="Prosperi, S" uniqKey="Prosperi S">S. Prosperi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berry, J D" uniqKey="Berry J">J.D. Berry</name></author><author><name sortKey="Hay, K" uniqKey="Hay K">K. Hay</name></author><author><name sortKey="Rini, J M" uniqKey="Rini J">J.M. Rini</name></author><author><name sortKey="Yu, M" uniqKey="Yu M">M. Yu</name></author><author><name sortKey="Want, L" uniqKey="Want L">L. Want</name></author><author><name sortKey="Plummer, F A" uniqKey="Plummer F">F.A. Plummer</name></author><author><name sortKey="Corbett, C R" uniqKey="Corbett C">C.R. Corbett</name></author><author><name sortKey="Andonov, A" uniqKey="Andonov A">A. Andonov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Van Der Zee, R" uniqKey="Van Der Zee R">R. van der Zee</name></author><author><name sortKey="De Haan, C A" uniqKey="De Haan C">C.A. de Haan</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Colman, P M" uniqKey="Colman P">P.M. Colman</name></author><author><name sortKey="Lawrence, M C" uniqKey="Lawrence M">M.C. Lawrence</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gallagher, T M" uniqKey="Gallagher T">T.M. Gallagher</name></author><author><name sortKey="Buchmeier, M J" uniqKey="Buchmeier M">M.J. Buchmeier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gimenez, L G" uniqKey="Gimenez L">L.G. Giménez</name></author><author><name sortKey="Rojas, J" uniqKey="Rojas J">J. Rojas</name></author><author><name sortKey="Rojas, A" uniqKey="Rojas A">A. Rojas</name></author><author><name sortKey="Mendoza, J" uniqKey="Mendoza J">J. Mendoza</name></author><author><name sortKey="Camacho, A G" uniqKey="Camacho A">A.G. Camacho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, Q" uniqKey="He Q">Q. He</name></author><author><name sortKey="Du, Q" uniqKey="Du Q">Q. Du</name></author><author><name sortKey="Lau, S" uniqKey="Lau S">S. Lau</name></author><author><name sortKey="Manopo, I" uniqKey="Manopo I">I. Manopo</name></author><author><name sortKey="Lu, L" uniqKey="Lu L">L. Lu</name></author><author><name sortKey="Chan, S W" uniqKey="Chan S">S.W. Chan</name></author><author><name sortKey="Fenner, B J" uniqKey="Fenner B">B.J. Fenner</name></author><author><name sortKey="Kwang, J" uniqKey="Kwang J">J. Kwang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, Y" uniqKey="He Y">Y. He</name></author><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Heck, S" uniqKey="Heck S">S. Heck</name></author><author><name sortKey="Lustigman, S" uniqKey="Lustigman S">S. Lustigman</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ho, T Y" uniqKey="Ho T">T.Y. Ho</name></author><author><name sortKey="Wu, S L" uniqKey="Wu S">S.L. Wu</name></author><author><name sortKey="Cheng, S E" uniqKey="Cheng S">S.E. Cheng</name></author><author><name sortKey="Wei, Y C" uniqKey="Wei Y">Y.C. Wei</name></author><author><name sortKey="Huang, S P" uniqKey="Huang S">S.P. Huang</name></author><author><name sortKey="Hsiang, C Y" uniqKey="Hsiang C">C.Y. Hsiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hofmann, H" uniqKey="Hofmann H">H. Hofmann</name></author><author><name sortKey="Geier, M" uniqKey="Geier M">M. Geier</name></author><author><name sortKey="Marzi, A" uniqKey="Marzi A">A. Marzi</name></author><author><name sortKey="Krumbiegel, M" uniqKey="Krumbiegel M">M. Krumbiegel</name></author><author><name sortKey="Peipp, M" uniqKey="Peipp M">M. Peipp</name></author><author><name sortKey="Fey, G H" uniqKey="Fey G">G.H. Fey</name></author><author><name sortKey="Gramberg, T" uniqKey="Gramberg T">T. Gramberg</name></author><author><name sortKey="Pohlmann, S" uniqKey="Pohlmann S">S. Pohlmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huanga, Y" uniqKey="Huanga Y">Y. Huanga</name></author><author><name sortKey="Zhaoa, R" uniqKey="Zhaoa R">R. Zhaoa</name></author><author><name sortKey="Jia Luoa, J" uniqKey="Jia Luoa J">J. Jia Luoa</name></author><author><name sortKey="Xionga, S" uniqKey="Xionga S">S. Xionga</name></author><author><name sortKey="Shangguana, D" uniqKey="Shangguana D">D. Shangguana</name></author><author><name sortKey="Zhanga, H" uniqKey="Zhanga H">H. Zhanga</name></author><author><name sortKey="Liua, G" uniqKey="Liua G">G. Liua</name></author><author><name sortKey="Chena, Y" uniqKey="Chena Y">Y. Chena</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, I C" uniqKey="Huang I">I.C. Huang</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Lee, K H" uniqKey="Lee K">K.H. Lee</name></author><author><name sortKey="Ghiran, S" uniqKey="Ghiran S">S. Ghiran</name></author><author><name sortKey="Vasilieva, N" uniqKey="Vasilieva N">N. Vasilieva</name></author><author><name sortKey="Dermody, T S" uniqKey="Dermody T">T.S. Dermody</name></author><author><name sortKey="Harrison, S C" uniqKey="Harrison S">S.C. Harrison</name></author><author><name sortKey="Dormitzer, P R" uniqKey="Dormitzer P">P.R. Dormitzer</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author><author><name sortKey="Choe, H" uniqKey="Choe H">H. Choe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kammila, S" uniqKey="Kammila S">S. Kammila</name></author><author><name sortKey="Das, D" uniqKey="Das D">D. Das</name></author><author><name sortKey="Bhatnagar, P K" uniqKey="Bhatnagar P">P.K. Bhatnagar</name></author><author><name sortKey="Sunwoo, H H" uniqKey="Sunwoo H">H.H. Sunwoo</name></author><author><name sortKey="Zayas Zamora, G" uniqKey="Zayas Zamora G">G. Zayas-Zamora</name></author><author><name sortKey="King, M" uniqKey="King M">M. King</name></author><author><name sortKey="Suresh, M R" uniqKey="Suresh M">M.R. Suresh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kohler, G" uniqKey="Kohler G">G. Kohler</name></author><author><name sortKey="Milstein, C" uniqKey="Milstein C">C. Milstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krokhin, O" uniqKey="Krokhin O">O. Krokhin</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Andonov, A" uniqKey="Andonov A">A. Andonov</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name></author><author><name sortKey="Flick, R" uniqKey="Flick R">R. Flick</name></author><author><name sortKey="Jones, S" uniqKey="Jones S">S. Jones</name></author><author><name sortKey="Stroeher, U" uniqKey="Stroeher U">U. Stroeher</name></author><author><name sortKey="Bastien, N" uniqKey="Bastien N">N. Bastien</name></author><author><name sortKey="Dasuri, K V" uniqKey="Dasuri K">K.V. Dasuri</name></author><author><name sortKey="Cheng, K" uniqKey="Cheng K">K. Cheng</name></author><author><name sortKey="Simonsen, J N" uniqKey="Simonsen J">J.N. Simonsen</name></author><author><name sortKey="Perreault, H" uniqKey="Perreault H">H. Perreault</name></author><author><name sortKey="Wilkins, J" uniqKey="Wilkins J">J. Wilkins</name></author><author><name sortKey="Ens, W" uniqKey="Ens W">W. Ens</name></author><author><name sortKey="Plummer, F" uniqKey="Plummer F">F. Plummer</name></author><author><name sortKey="Standing, K G" uniqKey="Standing K">K.G. Standing</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laemmli, U K" uniqKey="Laemmli U">U.K. Laemmli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, S K" uniqKey="Lau S">S.K. Lau</name></author><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author><author><name sortKey="Li, K S" uniqKey="Li K">K.S. Li</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y. Huang</name></author><author><name sortKey="Tsoi, H W" uniqKey="Tsoi H">H.W. Tsoi</name></author><author><name sortKey="Wong, B H" uniqKey="Wong B">B.H. Wong</name></author><author><name sortKey="Wong, S S" uniqKey="Wong S">S.S. Wong</name></author><author><name sortKey="Leung, S Y" uniqKey="Leung S">S.Y. Leung</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, G" uniqKey="Li G">G. Li</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X. Chen</name></author><author><name sortKey="Xu, A" uniqKey="Xu A">A. Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name></author><author><name sortKey="Harrison, S C" uniqKey="Harrison S">S.C. Harrison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Moore, M J" uniqKey="Moore M">M.J. Moore</name></author><author><name sortKey="Vasilieva, N" uniqKey="Vasilieva N">N. Vasilieva</name></author><author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name></author><author><name sortKey="Wong, S K" uniqKey="Wong S">S.K. Wong</name></author><author><name sortKey="Berne, M A" uniqKey="Berne M">M.A. Berne</name></author><author><name sortKey="Somasundaran, M" uniqKey="Somasundaran M">M. Somasundaran</name></author><author><name sortKey="Sullivan, J L" uniqKey="Sullivan J">J.L. Sullivan</name></author><author><name sortKey="Luzuriaga, K" uniqKey="Luzuriaga K">K. Luzuriaga</name></author><author><name sortKey="Greenough, T C" uniqKey="Greenough T">T.C. Greenough</name></author><author><name sortKey="Choe, H" uniqKey="Choe H">H. Choe</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, C K" uniqKey="Li C">C.K. Li</name></author><author><name sortKey="Wu, H" uniqKey="Wu H">H. Wu</name></author><author><name sortKey="Yan, H" uniqKey="Yan H">H. Yan</name></author><author><name sortKey="Ma, S" uniqKey="Ma S">S. Ma</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Zhang, M" uniqKey="Zhang M">M. Zhang</name></author><author><name sortKey="Tang, X" uniqKey="Tang X">X. Tang</name></author><author><name sortKey="Temperton, N J" uniqKey="Temperton N">N.J. Temperton</name></author><author><name sortKey="Weiss, R A" uniqKey="Weiss R">R.A. Weiss</name></author><author><name sortKey="Brenchley, J M" uniqKey="Brenchley J">J.M. Brenchley</name></author><author><name sortKey="Douek, D C" uniqKey="Douek D">D.C. Douek</name></author><author><name sortKey="Mongkolsapaya, J" uniqKey="Mongkolsapaya J">J. Mongkolsapaya</name></author><author><name sortKey="Tran, B H" uniqKey="Tran B">B.H. Tran</name></author><author><name sortKey="Lin, C L" uniqKey="Lin C">C.L. Lin</name></author><author><name sortKey="Screaton, G R" uniqKey="Screaton G">G.R. Screaton</name></author><author><name sortKey="Hou, J L" uniqKey="Hou J">J.L. Hou</name></author><author><name sortKey="Mcmichael, A J" uniqKey="Mcmichael A">A.J. McMichael</name></author><author><name sortKey="Xu, X N" uniqKey="Xu X">X.N. Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y. Chen</name></author><author><name sortKey="Bai, B" uniqKey="Bai B">B. Bai</name></author><author><name sortKey="Hu, H" uniqKey="Hu H">H. Hu</name></author><author><name sortKey="Tao, L" uniqKey="Tao L">L. Tao</name></author><author><name sortKey="Yang, J" uniqKey="Yang J">J. Yang</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J. Chen</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Hu, Z" uniqKey="Hu Z">Z. Hu</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, L" uniqKey="Lu L">L. Lu</name></author><author><name sortKey="Manopo, I" uniqKey="Manopo I">I. Manopo</name></author><author><name sortKey="Leung, B P" uniqKey="Leung B">B.P. Leung</name></author><author><name sortKey="Chng, H H" uniqKey="Chng H">H.H. Chng</name></author><author><name sortKey="Ling, A E" uniqKey="Ling A">A.E. Ling</name></author><author><name sortKey="Chee, L L" uniqKey="Chee L">L.L. Chee</name></author><author><name sortKey="Ooi, E E" uniqKey="Ooi E">E.E. Ooi</name></author><author><name sortKey="Chan, S W" uniqKey="Chan S">S.W. Chan</name></author><author><name sortKey="Kwang, J" uniqKey="Kwang J">J. Kwang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M.A. Marra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palaniyappan, A" uniqKey="Palaniyappan A">A. Palaniyappan</name></author><author><name sortKey="Das, D" uniqKey="Das D">D. Das</name></author><author><name sortKey="Kammila, S" uniqKey="Kammila S">S. Kammila</name></author><author><name sortKey="Suresh, M R" uniqKey="Suresh M">M.R. Suresh</name></author><author><name sortKey="Sunwoo, H H" uniqKey="Sunwoo H">H.H. Sunwoo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Wong, O K" uniqKey="Wong O">O.K. Wong</name></author><author><name sortKey="Yam, W C" uniqKey="Yam W">W.C. Yam</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author><author><name sortKey="Lo, Y M" uniqKey="Lo Y">Y.M. Lo</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P.A. Rota</name></author><author><name sortKey="Oberste, M S" uniqKey="Oberste M">M.S. Oberste</name></author><author><name sortKey="Monroe, S S" uniqKey="Monroe S">S.S. Monroe</name></author><author><name sortKey="Nix, W A" uniqKey="Nix W">W.A. Nix</name></author><author><name sortKey="Campagnoli, R" uniqKey="Campagnoli R">R. Campagnoli</name></author><author><name sortKey="Icenogle, J P" uniqKey="Icenogle J">J.P. Icenogle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sainz, B" uniqKey="Sainz B">B. Sainz</name></author><author><name sortKey="Rausch, J M" uniqKey="Rausch J">J.M. Rausch</name></author><author><name sortKey="Gallaher, W R" uniqKey="Gallaher W">W.R. Gallaher</name></author><author><name sortKey="Garry, R F" uniqKey="Garry R">R.F. Garry</name></author><author><name sortKey="Wimley, W C" uniqKey="Wimley W">W.C. Wimley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Siu, Y L" uniqKey="Siu Y">Y.L. Siu</name></author><author><name sortKey="Teoh, K T" uniqKey="Teoh K">K.T. Teoh</name></author><author><name sortKey="Lo, J" uniqKey="Lo J">J. Lo</name></author><author><name sortKey="Chan, C M" uniqKey="Chan C">C.M. Chan</name></author><author><name sortKey="Kien, F" uniqKey="Kien F">F. Kien</name></author><author><name sortKey="Escriou, N" uniqKey="Escriou N">N. Escriou</name></author><author><name sortKey="Tsao, S W" uniqKey="Tsao S">S.W. Tsao</name></author><author><name sortKey="Nicholls, J M" uniqKey="Nicholls J">J.M. Nicholls</name></author><author><name sortKey="Altmeyer, R" uniqKey="Altmeyer R">R. Altmeyer</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Bruzzone, R" uniqKey="Bruzzone R">R. Bruzzone</name></author><author><name sortKey="Nal, B" uniqKey="Nal B">B. Nal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Spiga, O" uniqKey="Spiga O">O. Spiga</name></author><author><name sortKey="Bernini, A" uniqKey="Bernini A">A. Bernini</name></author><author><name sortKey="Ciutti, A" uniqKey="Ciutti A">A. Ciutti</name></author><author><name sortKey="Chiellini, S" uniqKey="Chiellini S">S. Chiellini</name></author><author><name sortKey="Menciassi, N" uniqKey="Menciassi N">N. Menciassi</name></author><author><name sortKey="Finetti, F" uniqKey="Finetti F">F. Finetti</name></author><author><name sortKey="Causarono, V" uniqKey="Causarono V">V. Causarono</name></author><author><name sortKey="Anselmi, F" uniqKey="Anselmi F">F. Anselmi</name></author><author><name sortKey="Prischi, F" uniqKey="Prischi F">F. Prischi</name></author><author><name sortKey="Niccolai, N" uniqKey="Niccolai N">N. Niccolai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Murakami, A" uniqKey="Murakami A">A. Murakami</name></author><author><name sortKey="Tamin, A" uniqKey="Tamin A">A. Tamin</name></author><author><name sortKey="Matthews, L J" uniqKey="Matthews L">L.J. Matthews</name></author><author><name sortKey="Wong, S K" uniqKey="Wong S">S.K. Wong</name></author><author><name sortKey="Moore, M J" uniqKey="Moore M">M.J. Moore</name></author><author><name sortKey="Tallarico, A S" uniqKey="Tallarico A">A.S. Tallarico</name></author><author><name sortKey="Olurinde, M" uniqKey="Olurinde M">M. Olurinde</name></author><author><name sortKey="Choe, H" uniqKey="Choe H">H. Choe</name></author><author><name sortKey="Anderson, L J" uniqKey="Anderson L">L.J. Anderson</name></author><author><name sortKey="Bellini, W J" uniqKey="Bellini W">W.J. Bellini</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name></author><author><name sortKey="Marasco, W A" uniqKey="Marasco W">W.A. Marasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tan, Y J" uniqKey="Tan Y">Y.J. Tan</name></author><author><name sortKey="Goh, P Y" uniqKey="Goh P">P.Y. Goh</name></author><author><name sortKey="Fielding, B C" uniqKey="Fielding B">B.C. Fielding</name></author><author><name sortKey="Shen, S" uniqKey="Shen S">S. Shen</name></author><author><name sortKey="Chou, C F" uniqKey="Chou C">C.F. Chou</name></author><author><name sortKey="Fu, J L" uniqKey="Fu J">J.L. Fu</name></author><author><name sortKey="Leong, H N" uniqKey="Leong H">H.N. Leong</name></author><author><name sortKey="Leo, Y S" uniqKey="Leo Y">Y.S. Leo</name></author><author><name sortKey="Ooi, E E" uniqKey="Ooi E">E.E. Ooi</name></author><author><name sortKey="Ling, A E" uniqKey="Ling A">A.E. Ling</name></author><author><name sortKey="Lim, S G" uniqKey="Lim S">S.G. Lim</name></author><author><name sortKey="Hong, W" uniqKey="Hong W">W. Hong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, X L" uniqKey="Tang X">X.L. Tang</name></author><author><name sortKey="Peppler, M S" uniqKey="Peppler M">M.S. Peppler</name></author><author><name sortKey="Irvin, R T" uniqKey="Irvin R">R.T. Irvin</name></author><author><name sortKey="Suresh, M R" uniqKey="Suresh M">M.R. Suresh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Towbin, H" uniqKey="Towbin H">H. Towbin</name></author><author><name sortKey="Staehelin, T" uniqKey="Staehelin T">T. Staehelin</name></author><author><name sortKey="Gordon, J" uniqKey="Gordon J">J. Gordon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tripet, B" uniqKey="Tripet B">B. Tripet</name></author><author><name sortKey="Howard, M W" uniqKey="Howard M">M.W. Howard</name></author><author><name sortKey="Jobling, M" uniqKey="Jobling M">M. Jobling</name></author><author><name sortKey="Holmes, R K" uniqKey="Holmes R">R.K. Holmes</name></author><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name></author><author><name sortKey="Hodges, R S" uniqKey="Hodges R">R.S. Hodges</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Li, C" uniqKey="Li C">C. Li</name></author><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q. Zhang</name></author><author><name sortKey="Wang, T" uniqKey="Wang T">T. Wang</name></author><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Guan, W" uniqKey="Guan W">W. Guan</name></author><author><name sortKey="Yu, J" uniqKey="Yu J">J. Yu</name></author><author><name sortKey="Liang, M" uniqKey="Liang M">M. Liang</name></author><author><name sortKey="Li, D" uniqKey="Li D">D. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author><author><name sortKey="Lau, S K" uniqKey="Lau S">S.K. Lau</name></author><author><name sortKey="Wong, B H" uniqKey="Wong B">B.H. Wong</name></author><author><name sortKey="Tsoi, H W" uniqKey="Tsoi H">H.W. Tsoi</name></author><author><name sortKey="Fung, A M" uniqKey="Fung A">A.M. Fung</name></author><author><name sortKey="Kao, R Y" uniqKey="Kao R">R.Y. Kao</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiao, X" uniqKey="Xiao X">X. Xiao</name></author><author><name sortKey="Chakraborti, S" uniqKey="Chakraborti S">S. Chakraborti</name></author><author><name sortKey="Dimitrov, A S" uniqKey="Dimitrov A">A.S. Dimitrov</name></author><author><name sortKey="Gramatikoff, K" uniqKey="Gramatikoff K">K. Gramatikoff</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, X H" uniqKey="Yang X">X.H. Yang</name></author><author><name sortKey="Deng, W" uniqKey="Deng W">W. Deng</name></author><author><name sortKey="Tong, Z" uniqKey="Tong Z">Z. Tong</name></author><author><name sortKey="Liu, Y X" uniqKey="Liu Y">Y.X. Liu</name></author><author><name sortKey="Zhang, L F" uniqKey="Zhang L">L.F. Zhang</name></author><author><name sortKey="Zhu, H" uniqKey="Zhu H">H. Zhu</name></author><author><name sortKey="Gao, H" uniqKey="Gao H">H. Gao</name></author><author><name sortKey="Huang, L" uniqKey="Huang L">L. Huang</name></author><author><name sortKey="Liu, Y L" uniqKey="Liu Y">Y.L. Liu</name></author><author><name sortKey="Ma, C M" uniqKey="Ma C">C.M. Ma</name></author><author><name sortKey="Xu, Y F" uniqKey="Xu Y">Y.F. Xu</name></author><author><name sortKey="Ding, M X" uniqKey="Ding M">M.X. Ding</name></author><author><name sortKey="Deng, H K" uniqKey="Deng H">H.K. Deng</name></author><author><name sortKey="Qin, C" uniqKey="Qin C">C. Qin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ying, W" uniqKey="Ying W">W. Ying</name></author><author><name sortKey="Hao, Y" uniqKey="Hao Y">Y. Hao</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name></author><author><name sortKey="Peng, W" uniqKey="Peng W">W. Peng</name></author><author><name sortKey="Qin, E" uniqKey="Qin E">E. Qin</name></author><author><name sortKey="Cai, Y" uniqKey="Cai Y">Y. Cai</name></author><author><name sortKey="Wei, K" uniqKey="Wei K">K. Wei</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Chang, G" uniqKey="Chang G">G. Chang</name></author><author><name sortKey="Sun, W" uniqKey="Sun W">W. Sun</name></author><author><name sortKey="Dai, S" uniqKey="Dai S">S. Dai</name></author><author><name sortKey="Li, X" uniqKey="Li X">X. Li</name></author><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y. Zhu</name></author><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Wu, S" uniqKey="Wu S">S. Wu</name></author><author><name sortKey="Guo, L" uniqKey="Guo L">L. Guo</name></author><author><name sortKey="Dai, J" uniqKey="Dai J">J. Dai</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Wan, P" uniqKey="Wan P">P. Wan</name></author><author><name sortKey="Chen, T" uniqKey="Chen T">T. Chen</name></author><author><name sortKey="Du, C" uniqKey="Du C">C. Du</name></author><author><name sortKey="Li, D" uniqKey="Li D">D. Li</name></author><author><name sortKey="Wan, J" uniqKey="Wan J">J. Wan</name></author><author><name sortKey="Kuai, X" uniqKey="Kuai X">X. Kuai</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Shi, R" uniqKey="Shi R">R. Shi</name></author><author><name sortKey="Wei, H" uniqKey="Wei H">H. Wei</name></author><author><name sortKey="Cao, C" uniqKey="Cao C">C. Cao</name></author><author><name sortKey="Yu, M" uniqKey="Yu M">M. Yu</name></author><author><name sortKey="Liu, H" uniqKey="Liu H">H. Liu</name></author><author><name sortKey="Dong, F" uniqKey="Dong F">F. Dong</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X. Zhang</name></author><author><name sortKey="Qian, X" uniqKey="Qian X">X. Qian</name></author><author><name sortKey="Zhu, Q" uniqKey="Zhu Q">Q. Zhu</name></author><author><name sortKey="He, F" uniqKey="He F">F. He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yip, C W" uniqKey="Yip C">C.W. Yip</name></author><author><name sortKey="Hon, C C" uniqKey="Hon C">C.C. Hon</name></author><author><name sortKey="Shi, M" uniqKey="Shi M">M. Shi</name></author><author><name sortKey="Lam, T T" uniqKey="Lam T">T.T. Lam</name></author><author><name sortKey="Chow, K Y" uniqKey="Chow K">K.Y. Chow</name></author><author><name sortKey="Zeng, F" uniqKey="Zeng F">F. Zeng</name></author><author><name sortKey="Leung, F C" uniqKey="Leung F">F.C. Leung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, F" uniqKey="Yu F">F. Yu</name></author><author><name sortKey="Le, M Q" uniqKey="Le M">M.Q. Le</name></author><author><name sortKey="Inoue, S" uniqKey="Inoue S">S. Inoue</name></author><author><name sortKey="Hasebe, F" uniqKey="Hasebe F">F. Hasebe</name></author><author><name sortKey="Parquet" uniqKey="Parquet">Parquet</name></author><author><name sortKey="Mdel, C" uniqKey="Mdel C">C. Mdel</name></author><author><name sortKey="Morikawa, S" uniqKey="Morikawa S">S. Morikawa</name></author><author><name sortKey="Morita, K" uniqKey="Morita K">K. Morita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, J" uniqKey="Yuan J">J. Yuan</name></author><author><name sortKey="Hon, C C" uniqKey="Hon C">C.C. Hon</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Xu, G" uniqKey="Xu G">G. Xu</name></author><author><name sortKey="Zhang, H" uniqKey="Zhang H">H. Zhang</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P. Zhou</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Lam, T T" uniqKey="Lam T">T.T. Lam</name></author><author><name sortKey="Leung, F C" uniqKey="Leung F">F.C. Leung</name></author><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z. Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, T" uniqKey="Zhou T">T. Zhou</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Luo, D" uniqKey="Luo D">D. Luo</name></author><author><name sortKey="Rowe, T" uniqKey="Rowe T">T. Rowe</name></author><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z. Wang</name></author><author><name sortKey="Hogan, R J" uniqKey="Hogan R">R.J. Hogan</name></author><author><name sortKey="Qiu, S" uniqKey="Qiu S">S. Qiu</name></author><author><name sortKey="Bunzel, R J" uniqKey="Bunzel R">R.J. Bunzel</name></author><author><name sortKey="Huang, G" uniqKey="Huang G">G. Huang</name></author><author><name sortKey="Mishra, V" uniqKey="Mishra V">V. Mishra</name></author><author><name sortKey="Voss, T G" uniqKey="Voss T">T.G. Voss</name></author><author><name sortKey="Kimberly, R" uniqKey="Kimberly R">R. Kimberly</name></author><author><name sortKey="Luo, M" uniqKey="Luo M">M. Luo</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol Methods</journal-id><journal-id journal-id-type="iso-abbrev">J. Virol. Methods</journal-id><journal-title-group><journal-title>Journal of Virological Methods</journal-title></journal-title-group><issn pub-type="ppub">0166-0934</issn><issn pub-type="epub">1879-0984</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22995576</article-id><article-id pub-id-type="pmc">7112864</article-id><article-id pub-id-type="publisher-id">S0166-0934(12)00317-5</article-id><article-id pub-id-type="doi">10.1016/j.jviromet.2012.09.006</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Sunwoo</surname><given-names>Hoon H.</given-names></name><email>hsunwoo@ualberta.ca</email><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author"><name><surname>Palaniyappan</surname><given-names>Arivazhagan</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ganguly</surname><given-names>Advaita</given-names></name></contrib><contrib contrib-type="author"><name><surname>Bhatnagar</surname><given-names>Pravin K.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Das</surname><given-names>Dipankar</given-names></name></contrib><contrib contrib-type="author"><name><surname>El-Kadi</surname><given-names>Ayman O.S.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Suresh</surname><given-names>Mavanur R.</given-names></name></contrib></contrib-group><aff>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E1</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author at: 3142G Katz Group Centre for Pharmacy and Health Research, Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11361 – 87 Avenue, Edmonton, Alberta, Canada T6G 2E1. Tel.: +1 780 492 0547; fax: +1 780 492 1217. <email>hsunwoo@ualberta.ca</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>17</day><month>9</month><year>2012</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>1</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>17</day><month>9</month><year>2012</year></pub-date><volume>187</volume><issue>1</issue><fpage>72</fpage><lpage>78</lpage><history><date date-type="received"><day>22</day><month>3</month><year>2012</year></date><date date-type="rev-recd"><day>21</day><month>8</month><year>2012</year></date><date date-type="accepted"><day>5</day><month>9</month><year>2012</year></date></history><permissions><copyright-statement>Copyright © 2012 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2012</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract abstract-type="graphical"><title>Highlights</title><p>► Two monoclonal antibodies against SARS CoV S1 antigen were generated by hybridomatechnology. ► Bispecific antibody (bsmAb) was developed by Quadroma technology. ► Mono- and bispecific antibodies were evaluated by sandiwichELISA for immunodiagnosis. ► We report that bsmAb-based ELISA is more sensitive than the mAb-based ELISA for detection of the S1 antigen.</p></abstract><abstract><p>The severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein is known to mediate receptor interaction and immune recognition and thus it is considered as a major target for vaccine design. The spike protein plays an important role in virus entry, virus receptor interactions, and virus tropism. Sensitive diagnosis of SARS is essential for the control of the disease in humans. Recombinant SARS-CoV S1 antigen was produced and purified for the development of monoclonal and bi-specific monoclonal antibodies. The hybridomas secreting anti-S1 antibodies, F26G18 and P136.8D12, were fused respectively with the YP4 hybridoma to generate quadromas. The sandwich ELISA was formed by using F26G18 as a coating antibody and biotinylated F26G18 as a detection antibody with a detection limit of 0.037 μg/ml (<italic>p</italic> < 0.02). The same detection limit was found with P136.8D12 as a coating antibody and biotinylated F26G18 as a detection antibody. The sensitivity was improved (detection limit of 0.019 μg/ml), however, when using bi-specific monoclonal antibody (F157) as the detection antibody. In conclusion, the method described in this study allows sensitive detection of a recombinant SARS spike protein by sandwich ELISA with bi-specific monoclonal antibody and could be used for the diagnosis of patients suspected with SARS.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS-CoV</kwd><kwd>Spike protein</kwd><kwd>Bi-specific monoclonal antibodies</kwd><kwd>Quadroma</kwd><kwd>ELISA</kwd></kwd-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Bhatnagar, Pravin K" sort="Bhatnagar, Pravin K" uniqKey="Bhatnagar P" first="Pravin K." last="Bhatnagar">Pravin K. Bhatnagar</name><name sortKey="Das, Dipankar" sort="Das, Dipankar" uniqKey="Das D" first="Dipankar" last="Das">Dipankar Das</name><name sortKey="El Kadi, Ayman O S" sort="El Kadi, Ayman O S" uniqKey="El Kadi A" first="Ayman O. S." last="El-Kadi">Ayman O. S. El-Kadi</name><name sortKey="Ganguly, Advaita" sort="Ganguly, Advaita" uniqKey="Ganguly A" first="Advaita" last="Ganguly">Advaita Ganguly</name><name sortKey="Palaniyappan, Arivazhagan" sort="Palaniyappan, Arivazhagan" uniqKey="Palaniyappan A" first="Arivazhagan" last="Palaniyappan">Arivazhagan Palaniyappan</name><name sortKey="Sunwoo, Hoon H" sort="Sunwoo, Hoon H" uniqKey="Sunwoo H" first="Hoon H." last="Sunwoo">Hoon H. Sunwoo</name><name sortKey="Suresh, Mavanur R" sort="Suresh, Mavanur R" uniqKey="Suresh M" first="Mavanur R." last="Suresh">Mavanur R. Suresh</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A40 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000A40 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:7112864
|texte= Quantitative and sensitive detection of the SARS-CoV spike protein using bispecific monoclonal antibody-based enzyme-linked immunoassay
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:22995576" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |