SrasV1, Main, Exploration, bibRecordById, PMC:446234

Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing

Identifieur interne : 005B26 ( Main/Exploration ); précédent : 005B25; suivant : 005B27

Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing

Auteurs : T. Vinayagamoorthy [Canada] ; Kirk Mulatz [Canada] ; Roger Hodkinson [Canada]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.

RBID : Pascal:04-0407702

Descripteurs français

KwdFr :
- Analyse de séquence d'ADN, Plasmides, Réaction de polymérisation en chaîne, Transformation génétique, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
MESH :
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
Pascal (Inist)
- Analyse de séquence d'ADN, Coronavirus, Identification, Plasmides, Réaction de polymérisation en chaîne, Séquençage, Microbiologie, Syndrome respiratoire aigu sévère, Transformation génétique.

English descriptors

KwdEn :
- Coronavirus, Identification, Microbiology, Plasmids, Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Sequence Analysis, DNA, Sequencing, Severe acute respiratory syndrome, Transformation, Genetic.
MESH :
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- Plasmids, Polymerase Chain Reaction, Sequence Analysis, DNA, Transformation, Genetic.

Abstract

The recent severe acute respiratory syndrome (SARS) outbreak resulted in calls for an accurate diagnostic test that can be used not only for routine testing but also for generating nucleotide sequences to monitor the epidemic. Although the identity of the SARS coronavirus (SARS-CoV) genome was confirmed by DNA sequencing, it is impractical to sequence the entire 29-kb SARS-CoV genome on a routine basis. Therefore, alternative assay methods such as the enzyme-linked immunosorbent assay and PCR have been pursued for routine testing, primarily to resolve probable cases. We report here a modification of standard DNA sequencing technology for accurate identification of SARS-CoV in routine testing. Instead of requiring the sequencing of the whole SARS-CoV genome, our modification enables the simultaneous sequencing of three regions of the SARS-CoV genome, the spike protein-encoding gene (35 nucleotides), gene M (43 nucleotides), and gene N (45 nucleotides), in a single electropherogram. Comparing these nucleotide sequences to DNA databank entries (National Institutes of Health) conclusively identified them as SARS-CoV sequences.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC446234

Affiliations:

Canada

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<front><div type="abstract" xml:lang="en">The recent severe acute respiratory syndrome (SARS) outbreak resulted in calls for an accurate diagnostic test that can be used not only for routine testing but also for generating nucleotide sequences to monitor the epidemic. Although the identity of the SARS coronavirus (SARS-CoV) genome was confirmed by DNA sequencing, it is impractical to sequence the entire 29-kb SARS-CoV genome on a routine basis. Therefore, alternative assay methods such as the enzyme-linked immunosorbent assay and PCR have been pursued for routine testing, primarily to resolve probable cases. We report here a modification of standard DNA sequencing technology for accurate identification of SARS-CoV in routine testing. Instead of requiring the sequencing of the whole SARS-CoV genome, our modification enables the simultaneous sequencing of three regions of the SARS-CoV genome, the spike protein-encoding gene (35 nucleotides), gene M (43 nucleotides), and gene N (45 nucleotides), in a single electropherogram. Comparing these nucleotide sequences to DNA databank entries (National Institutes of Health) conclusively identified them as SARS-CoV sequences.</div>
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Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing

Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration SRAS
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