Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing
Identifieur interne : 005F87 ( Main/Merge ); précédent : 005F86; suivant : 005F88Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing
Auteurs : T. Vinayagamoorthy [Canada] ; Kirk Mulatz [Canada] ; Roger Hodkinson [Canada]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
The recent severe acute respiratory syndrome (SARS) outbreak resulted in calls for an accurate diagnostic test that can be used not only for routine testing but also for generating nucleotide sequences to monitor the epidemic. Although the identity of the SARS coronavirus (SARS-CoV) genome was confirmed by DNA sequencing, it is impractical to sequence the entire 29-kb SARS-CoV genome on a routine basis. Therefore, alternative assay methods such as the enzyme-linked immunosorbent assay and PCR have been pursued for routine testing, primarily to resolve probable cases. We report here a modification of standard DNA sequencing technology for accurate identification of SARS-CoV in routine testing. Instead of requiring the sequencing of the whole SARS-CoV genome, our modification enables the simultaneous sequencing of three regions of the SARS-CoV genome, the spike protein-encoding gene (35 nucleotides), gene M (43 nucleotides), and gene N (45 nucleotides), in a single electropherogram. Comparing these nucleotide sequences to DNA databank entries (National Institutes of Health) conclusively identified them as SARS-CoV sequences.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000840
- to stream PascalFrancis, to step Curation: 000150
- to stream PascalFrancis, to step Checkpoint: 000836
Links to Exploration step
Pascal:04-0407702Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing</title>
<author><name sortKey="Vinayagamoorthy, T" sort="Vinayagamoorthy, T" uniqKey="Vinayagamoorthy T" first="T." last="Vinayagamoorthy">T. Vinayagamoorthy</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Bio-ID Diagnostic Inc.</s1>
<s2>Saskatoon, Saskatchewan, S7N 4N1</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Bio-ID Diagnostic Inc.</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mulatz, Kirk" sort="Mulatz, Kirk" uniqKey="Mulatz K" first="Kirk" last="Mulatz">Kirk Mulatz</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Bio-ID Diagnostic Inc.</s1>
<s2>Saskatoon, Saskatchewan, S7N 4N1</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Bio-ID Diagnostic Inc.</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hodkinson, Roger" sort="Hodkinson, Roger" uniqKey="Hodkinson R" first="Roger" last="Hodkinson">Roger Hodkinson</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Bio-ID Diagnostic Inc.</s1>
<s2>Saskatoon, Saskatchewan, S7N 4N1</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Bio-ID Diagnostic Inc.</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">04-0407702</idno>
<date when="2004">2004</date>
<idno type="stanalyst">PASCAL 04-0407702 INIST</idno>
<idno type="RBID">Pascal:04-0407702</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000840</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000150</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000836</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000836</idno>
<idno type="wicri:doubleKey">0095-1137:2004:Vinayagamoorthy T:identification:of:the</idno>
<idno type="wicri:Area/Main/Merge">005F87</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing</title>
<author><name sortKey="Vinayagamoorthy, T" sort="Vinayagamoorthy, T" uniqKey="Vinayagamoorthy T" first="T." last="Vinayagamoorthy">T. Vinayagamoorthy</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Bio-ID Diagnostic Inc.</s1>
<s2>Saskatoon, Saskatchewan, S7N 4N1</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Bio-ID Diagnostic Inc.</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mulatz, Kirk" sort="Mulatz, Kirk" uniqKey="Mulatz K" first="Kirk" last="Mulatz">Kirk Mulatz</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Bio-ID Diagnostic Inc.</s1>
<s2>Saskatoon, Saskatchewan, S7N 4N1</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Bio-ID Diagnostic Inc.</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hodkinson, Roger" sort="Hodkinson, Roger" uniqKey="Hodkinson R" first="Roger" last="Hodkinson">Roger Hodkinson</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Bio-ID Diagnostic Inc.</s1>
<s2>Saskatoon, Saskatchewan, S7N 4N1</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Bio-ID Diagnostic Inc.</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Identification</term>
<term>Microbiology</term>
<term>Sequencing</term>
<term>Severe acute respiratory syndrome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Identification</term>
<term>Séquençage</term>
<term>Microbiologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The recent severe acute respiratory syndrome (SARS) outbreak resulted in calls for an accurate diagnostic test that can be used not only for routine testing but also for generating nucleotide sequences to monitor the epidemic. Although the identity of the SARS coronavirus (SARS-CoV) genome was confirmed by DNA sequencing, it is impractical to sequence the entire 29-kb SARS-CoV genome on a routine basis. Therefore, alternative assay methods such as the enzyme-linked immunosorbent assay and PCR have been pursued for routine testing, primarily to resolve probable cases. We report here a modification of standard DNA sequencing technology for accurate identification of SARS-CoV in routine testing. Instead of requiring the sequencing of the whole SARS-CoV genome, our modification enables the simultaneous sequencing of three regions of the SARS-CoV genome, the spike protein-encoding gene (35 nucleotides), gene M (43 nucleotides), and gene N (45 nucleotides), in a single electropherogram. Comparing these nucleotide sequences to DNA databank entries (National Institutes of Health) conclusively identified them as SARS-CoV sequences.</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
</country>
</list>
<tree><country name="Canada"><noRegion><name sortKey="Vinayagamoorthy, T" sort="Vinayagamoorthy, T" uniqKey="Vinayagamoorthy T" first="T." last="Vinayagamoorthy">T. Vinayagamoorthy</name>
</noRegion>
<name sortKey="Hodkinson, Roger" sort="Hodkinson, Roger" uniqKey="Hodkinson R" first="Roger" last="Hodkinson">Roger Hodkinson</name>
<name sortKey="Mulatz, Kirk" sort="Mulatz, Kirk" uniqKey="Mulatz K" first="Kirk" last="Mulatz">Kirk Mulatz</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005F87 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 005F87 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Merge |type= RBID |clé= Pascal:04-0407702 |texte= Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing }}
This area was generated with Dilib version V0.6.33. |