SrasV1, PascalFrancis, Curation, bibRecord, 000150

Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing

Identifieur interne : 000150 ( PascalFrancis/Curation ); précédent : 000149; suivant : 000151

Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing

Auteurs : T. Vinayagamoorthy [Canada] ; Kirk Mulatz [Canada] ; Roger Hodkinson [Canada]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.

RBID : Pascal:04-0407702

Descripteurs français

Pascal (Inist)
- Coronavirus, Identification, Séquençage, Microbiologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Identification, Microbiology, Sequencing, Severe acute respiratory syndrome.

Abstract

The recent severe acute respiratory syndrome (SARS) outbreak resulted in calls for an accurate diagnostic test that can be used not only for routine testing but also for generating nucleotide sequences to monitor the epidemic. Although the identity of the SARS coronavirus (SARS-CoV) genome was confirmed by DNA sequencing, it is impractical to sequence the entire 29-kb SARS-CoV genome on a routine basis. Therefore, alternative assay methods such as the enzyme-linked immunosorbent assay and PCR have been pursued for routine testing, primarily to resolve probable cases. We report here a modification of standard DNA sequencing technology for accurate identification of SARS-CoV in routine testing. Instead of requiring the sequencing of the whole SARS-CoV genome, our modification enables the simultaneous sequencing of three regions of the SARS-CoV genome, the spike protein-encoding gene (35 nucleotides), gene M (43 nucleotides), and gene N (45 nucleotides), in a single electropherogram. Comparing these nucleotide sequences to DNA databank entries (National Institutes of Health) conclusively identified them as SARS-CoV sequences.

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A02	`01`			`@0 JCMIDW`
A03		`1`		`@0 J. clin. microbiol. : (Print)`
A05				`@2 42`
A06				`@2 7`
A08	`01`	`1`	`ENG`	`@1 Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing`
A11	`01`	`1`		`@1 VINAYAGAMOORTHY (T.)`
A11	`02`	`1`		`@1 MULATZ (Kirk)`
A11	`03`	`1`		`@1 HODKINSON (Roger)`
A14	`01`			`@1 Bio-ID Diagnostic Inc. @2 Saskatoon, Saskatchewan, S7N 4N1 @3 CAN @Z 1 aut. @Z 2 aut. @Z 3 aut.`
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A44				`@0 0000 @1 © 2004 INIST-CNRS. All rights reserved.`
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A61				`@0 A`
A64	`01`	`1`		`@0 Journal of clinical microbiology : (Print)`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 The recent severe acute respiratory syndrome (SARS) outbreak resulted in calls for an accurate diagnostic test that can be used not only for routine testing but also for generating nucleotide sequences to monitor the epidemic. Although the identity of the SARS coronavirus (SARS-CoV) genome was confirmed by DNA sequencing, it is impractical to sequence the entire 29-kb SARS-CoV genome on a routine basis. Therefore, alternative assay methods such as the enzyme-linked immunosorbent assay and PCR have been pursued for routine testing, primarily to resolve probable cases. We report here a modification of standard DNA sequencing technology for accurate identification of SARS-CoV in routine testing. Instead of requiring the sequencing of the whole SARS-CoV genome, our modification enables the simultaneous sequencing of three regions of the SARS-CoV genome, the spike protein-encoding gene (35 nucleotides), gene M (43 nucleotides), and gene N (45 nucleotides), in a single electropherogram. Comparing these nucleotide sequences to DNA databank entries (National Institutes of Health) conclusively identified them as SARS-CoV sequences.
C02	`01`	`X`		`@0 002A05C10`
C02	`02`	`X`		`@0 002B05`
C03	`01`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Identification @5 05`
C03	`02`	`X`	`ENG`	`@0 Identification @5 05`
C03	`02`	`X`	`SPA`	`@0 Identificación @5 05`
C03	`03`	`X`	`FRE`	`@0 Séquençage @5 06`
C03	`03`	`X`	`ENG`	`@0 Sequencing @5 06`
C03	`03`	`X`	`SPA`	`@0 Sequencing @5 06`
C03	`04`	`X`	`FRE`	`@0 Microbiologie @5 07`
C03	`04`	`X`	`ENG`	`@0 Microbiology @5 07`
C03	`04`	`X`	`SPA`	`@0 Microbiología @5 07`
C03	`05`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
C03	`05`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`05`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
C07	`01`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`03`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`03`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`04`	`X`	`FRE`	`@0 Virose @2 NM`
C07	`04`	`X`	`ENG`	`@0 Viral disease @2 NM`
C07	`04`	`X`	`SPA`	`@0 Virosis @2 NM`
C07	`05`	`X`	`FRE`	`@0 Infection @2 NM`
C07	`05`	`X`	`ENG`	`@0 Infection @2 NM`
C07	`05`	`X`	`SPA`	`@0 Infección @2 NM`
C07	`06`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 19`
C07	`06`	`X`	`ENG`	`@0 Respiratory disease @5 19`
C07	`06`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 19`
C07	`07`	`X`	`FRE`	`@0 Poumon pathologie @5 20`
C07	`07`	`X`	`ENG`	`@0 Lung disease @5 20`
C07	`07`	`X`	`SPA`	`@0 Pulmón patología @5 20`
N21				`@1 229`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

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Pascal:04-0407702

Le document en format XML

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Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing

Identification of the severe acute respiratory syndrome coronavirus by simultaneous multigene DNA sequencing

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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