SrasV1, Main, Exploration, bibRecord, 005A69

Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction

Identifieur interne : 005A69 ( Main/Exploration ); précédent : 005A68; suivant : 005A70

Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction

Auteurs : SHIH SHENG JIANG [Taïwan] ; Tsan-Chi Chen [Taïwan] ; Jyh-Yuan Yang [Taïwan] ; Chao A. Hsiung [Taïwan] ; Ih-Jen Su [Taïwan] ; Ying-Lan Liu [Taïwan] ; Po-Cheng Chen [Taïwan] ; Jyh-Lyh Juang [Taïwan]

Source :

Clinical infectious diseases [ 1058-4838 ] ; 2004.

RBID : Pascal:04-0570616

Descripteurs français

Pascal (Inist)
- Syndrome respiratoire aigu sévère, Réaction chaîne polymérase, Analyse quantitative, Réaction chaîne polymérase nichée, Méthode, Coronavirus, Malade état grave, Sensibilité, Appareillage, Dépistage, Aigu, Etude comparative, RNA-directed DNA polymerase, Homme, Forme grave, Reverse transcriptase.
Wicri :
- topic : Analyse quantitative, Homme.

English descriptors

KwdEn :
- Acute, Comparative study, Coronavirus, Critically ill, Human, Instrumentation, Medical screening, Method, Nested polymerase chain reaction, Polymerase chain reaction, Quantitative analysis, RNA-directed DNA polymerase, Sensitivity, Severe acute respiratory syndrome.

Abstract

A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.

Affiliations:

Taïwan

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000772
to stream PascalFrancis, to step Curation: 000218
to stream PascalFrancis, to step Checkpoint: 000773
to stream Main, to step Merge: 005F24
to stream Main, to step Curation: 005A69

Le document en format XML

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<front><div type="abstract" xml:lang="en">A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.</div>
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Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction

Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri