SrasV1, PascalFrancis, Curation, bibRecord, 000218

Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction

Identifieur interne : 000218 ( PascalFrancis/Curation ); précédent : 000217; suivant : 000219

Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction

Auteurs : SHIH SHENG JIANG [Taïwan] ; Tsan-Chi Chen [Taïwan] ; Jyh-Yuan Yang [Taïwan] ; Chao A. Hsiung [Taïwan] ; Ih-Jen Su [Taïwan] ; Ying-Lan Liu [Taïwan] ; Po-Cheng Chen [Taïwan] ; Jyh-Lyh Juang [Taïwan]

Source :

Clinical infectious diseases [ 1058-4838 ] ; 2004.

RBID : Pascal:04-0570616

Descripteurs français

Pascal (Inist)
- Syndrome respiratoire aigu sévère, Réaction chaîne polymérase, Analyse quantitative, Réaction chaîne polymérase nichée, Méthode, Coronavirus, Malade état grave, Sensibilité, Appareillage, Dépistage, Aigu, Etude comparative, RNA-directed DNA polymerase, Homme, Forme grave, Reverse transcriptase.
Wicri :
- topic : Analyse quantitative, Homme.

English descriptors

KwdEn :
- Acute, Comparative study, Coronavirus, Critically ill, Human, Instrumentation, Medical screening, Method, Nested polymerase chain reaction, Polymerase chain reaction, Quantitative analysis, RNA-directed DNA polymerase, Sensitivity, Severe acute respiratory syndrome.

Abstract

A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.

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A11	`01`	`1`		`@1 SHIH SHENG JIANG`
A11	`02`	`1`		`@1 CHEN (Tsan-Chi)`
A11	`03`	`1`		`@1 YANG (Jyh-Yuan)`
A11	`04`	`1`		`@1 HSIUNG (Chao A.)`
A11	`05`	`1`		`@1 SU (Ih-Jen)`
A11	`06`	`1`		`@1 LIU (Ying-Lan)`
A11	`07`	`1`		`@1 CHEN (Po-Cheng)`
A11	`08`	`1`		`@1 JUANG (Jyh-Lyh)`
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A14	`03`			`@1 Division of Laboratory Research and Development, Center for Disease Control, Department of Health @2 Taipei @3 TWN @Z 5 aut.`
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C01	`01`		`ENG`	@0 A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.
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C03	`04`	`X`	`FRE`	`@0 Réaction chaîne polymérase nichée @5 08`
C03	`04`	`X`	`ENG`	`@0 Nested polymerase chain reaction @5 08`
C03	`04`	`X`	`SPA`	`@0 Reacción cadena polimerasa anidada @5 08`
C03	`05`	`X`	`FRE`	`@0 Méthode @5 09`
C03	`05`	`X`	`ENG`	`@0 Method @5 09`
C03	`05`	`X`	`SPA`	`@0 Método @5 09`
C03	`06`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 10`
C03	`06`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 10`
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C03	`07`	`X`	`FRE`	`@0 Malade état grave @5 11`
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C03	`08`	`X`	`SPA`	`@0 Sensibilidad @5 13`
C03	`09`	`X`	`FRE`	`@0 Appareillage @5 14`
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C03	`10`	`X`	`FRE`	`@0 Dépistage @5 15`
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C07	`06`	`X`	`FRE`	`@0 Nucleotidyltransferases @2 FE`
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C07	`07`	`X`	`FRE`	`@0 Transferases @2 FE`
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Le document en format XML

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<series><title level="j" type="main">Clinical infectious diseases</title>
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<front><div type="abstract" xml:lang="en">A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.</div>
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<fA06><s2>2</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>SHIH SHENG JIANG</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>CHEN (Tsan-Chi)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>YANG (Jyh-Yuan)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>HSIUNG (Chao A.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>SU (Ih-Jen)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>LIU (Ying-Lan)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>CHEN (Po-Cheng)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>JUANG (Jyh-Lyh)</s1>
</fA11>
<fA14 i1="01"><s1>Division of Molecular and Genomic Medicine, National Health Research Institutes</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Division of Biostatistics and Bioinformatics, National Research Institutes</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Division of Laboratory Research and Development, Center for Disease Control, Department of Health</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA20><s1>293-299</s1>
</fA20>
<fA21><s1>2004</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>18407</s2>
<s5>354000111691750170</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2004 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>34 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>04-0570616</s0>
</fA47>
<fA60><s1>P</s1>
<s3>AR</s3>
<s3>CT</s3>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Clinical infectious diseases</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B05C02C</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Réaction chaîne polymérase</s0>
<s5>04</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Polymerase chain reaction</s0>
<s5>04</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Reacción cadena polimerasa</s0>
<s5>04</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Analyse quantitative</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Quantitative analysis</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Análisis cuantitativo</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Réaction chaîne polymérase nichée</s0>
<s5>08</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Nested polymerase chain reaction</s0>
<s5>08</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Reacción cadena polimerasa anidada</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Méthode</s0>
<s5>09</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Method</s0>
<s5>09</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Método</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>10</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>10</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Malade état grave</s0>
<s5>11</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Critically ill</s0>
<s5>11</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Enfermo estado grave</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Sensibilité</s0>
<s5>13</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Sensitivity</s0>
<s5>13</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Sensibilidad</s0>
<s5>13</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Appareillage</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Instrumentation</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Instrumentación</s0>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Dépistage</s0>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Medical screening</s0>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Descubrimiento</s0>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Aigu</s0>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Acute</s0>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Agudo</s0>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Etude comparative</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Comparative study</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Estudio comparativo</s0>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Homme</s0>
<s5>25</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Human</s0>
<s5>25</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Hombre</s0>
<s5>25</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Forme grave</s0>
<s4>INC</s4>
<s5>86</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Reverse transcriptase</s0>
<s4>INC</s4>
<s5>87</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Viral disease</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Virosis</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>37</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>37</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>37</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>38</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>38</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>38</s5>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Biologie moléculaire</s0>
<s5>40</s5>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Molecular biology</s0>
<s5>40</s5>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Biología molecular</s0>
<s5>40</s5>
</fC07>
<fN21><s1>327</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction

Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction

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