Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction
Identifieur interne : 005A69 ( Main/Curation ); précédent : 005A68; suivant : 005A70Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction
Auteurs : SHIH SHENG JIANG [Taïwan] ; Tsan-Chi Chen [Taïwan] ; Jyh-Yuan Yang [Taïwan] ; Chao A. Hsiung [Taïwan] ; Ih-Jen Su [Taïwan] ; Ying-Lan Liu [Taïwan] ; Po-Cheng Chen [Taïwan] ; Jyh-Lyh Juang [Taïwan]Source :
- Clinical infectious diseases [ 1058-4838 ] ; 2004.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Analyse quantitative, Homme.
English descriptors
- KwdEn :
Abstract
A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.
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<term>Human</term>
<term>Instrumentation</term>
<term>Medical screening</term>
<term>Method</term>
<term>Nested polymerase chain reaction</term>
<term>Polymerase chain reaction</term>
<term>Quantitative analysis</term>
<term>RNA-directed DNA polymerase</term>
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<term>Méthode</term>
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<term>Sensibilité</term>
<term>Appareillage</term>
<term>Dépistage</term>
<term>Aigu</term>
<term>Etude comparative</term>
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<term>Forme grave</term>
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<front><div type="abstract" xml:lang="en">A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.</div>
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