Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination
Identifieur interne : 004E26 ( Main/Exploration ); précédent : 004E25; suivant : 004E27Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination
Auteurs : HUADONG PEI [République populaire de Chine] ; JINGFANG LIU [République populaire de Chine] ; YUN CHENG [République populaire de Chine] ; CHAOMIN SUN [République populaire de Chine] ; CHEN WANG [République populaire de Chine] ; YUEPING LU [République populaire de Chine] ; JIE DING [République populaire de Chine] ; JIAN ZHOU [République populaire de Chine] ; HUA XIANG [République populaire de Chine]Source :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2005.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Vaccination.
English descriptors
- KwdEn :
Abstract
The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.
Affiliations:
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Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Diagnosis</term>
<term>Escherichia coli</term>
<term>Gene expression</term>
<term>Lactococcus lactis</term>
<term>Nucleocapsid</term>
<term>Protein</term>
<term>Recombinant protein</term>
<term>Severe acute respiratory syndrome</term>
<term>Vaccination</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Nucléocapside</term>
<term>Protéine</term>
<term>Escherichia coli</term>
<term>Lactococcus lactis</term>
<term>Diagnostic</term>
<term>Coronavirus</term>
<term>Vaccination</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Expression génique</term>
<term>Protéine recombinante</term>
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<front><div type="abstract" xml:lang="en">The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.</div>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Huadong Pei" sort="Huadong Pei" uniqKey="Huadong Pei" last="Huadong Pei">HUADONG PEI</name>
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<name sortKey="Hua Xiang" sort="Hua Xiang" uniqKey="Hua Xiang" last="Hua Xiang">HUA XIANG</name>
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<name sortKey="Jingfang Liu" sort="Jingfang Liu" uniqKey="Jingfang Liu" last="Jingfang Liu">JINGFANG LIU</name>
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<name sortKey="Yueping Lu" sort="Yueping Lu" uniqKey="Yueping Lu" last="Yueping Lu">YUEPING LU</name>
<name sortKey="Yun Cheng" sort="Yun Cheng" uniqKey="Yun Cheng" last="Yun Cheng">YUN CHENG</name>
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