SrasV1, PascalFrancis, Checkpoint, bibRecord, 000649

Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination

Identifieur interne : 000649 ( PascalFrancis/Checkpoint ); précédent : 000648; suivant : 000650

Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination

Auteurs : HUADONG PEI [République populaire de Chine] ; JINGFANG LIU [République populaire de Chine] ; YUN CHENG [République populaire de Chine] ; CHAOMIN SUN [République populaire de Chine] ; CHEN WANG [République populaire de Chine] ; YUEPING LU [République populaire de Chine] ; JIE DING [République populaire de Chine] ; JIAN ZHOU [République populaire de Chine] ; HUA XIANG [République populaire de Chine]

Source :

Applied microbiology and biotechnology [ 0175-7598 ] ; 2005.

RBID : Pascal:06-0009448

Descripteurs français

Pascal (Inist)
- Nucléocapside, Protéine, Escherichia coli, Lactococcus lactis, Diagnostic, Coronavirus, Vaccination, Syndrome respiratoire aigu sévère, Expression génique, Protéine recombinante.
Wicri :
- topic : Vaccination.

English descriptors

KwdEn :
- Coronavirus, Diagnosis, Escherichia coli, Gene expression, Lactococcus lactis, Nucleocapsid, Protein, Recombinant protein, Severe acute respiratory syndrome, Vaccination.

Abstract

The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.

Affiliations:

République populaire de Chine

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Le document en format XML

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<term>Diagnosis</term>
<term>Escherichia coli</term>
<term>Gene expression</term>
<term>Lactococcus lactis</term>
<term>Nucleocapsid</term>
<term>Protein</term>
<term>Recombinant protein</term>
<term>Severe acute respiratory syndrome</term>
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<term>Coronavirus</term>
<term>Vaccination</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Expression génique</term>
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<front><div type="abstract" xml:lang="en">The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.</div>
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<fA23 i1="01"><s0>ENG</s0>
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<fA43 i1="01"><s1>INIST</s1>
<s2>16771</s2>
<s5>354000131512620100</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2006 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>20 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>06-0009448</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Applied microbiology and biotechnology</s0>
</fA64>
<fA66 i1="01"><s0>DEU</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A31D01G</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Nucléocapside</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Nucleocapsid</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Nucleocápside</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Protéine</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Protein</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Proteína</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Lactococcus lactis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Lactococcus lactis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Lactococcus lactis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Diagnostic</s0>
<s5>12</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Diagnosis</s0>
<s5>12</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Diagnóstico</s0>
<s5>12</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Vaccination</s0>
<s5>19</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Vaccination</s0>
<s5>19</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Vacunación</s0>
<s5>19</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>20</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>20</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>20</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Expression génique</s0>
<s5>21</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Gene expression</s0>
<s5>21</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Expresión genética</s0>
<s5>21</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Protéine recombinante</s0>
<s5>22</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Recombinant protein</s0>
<s5>22</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Proteína recombinante</s0>
<s5>22</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Streptococcaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Streptococcaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Streptococcaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Micrococcales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Micrococcales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Micrococcales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Bactérie lactique</s0>
<s5>23</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Lactic acid bacteria</s0>
<s5>23</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Bacteria láctica</s0>
<s5>23</s5>
</fC07>
<fN21><s1>002</s1>
</fN21>
</pA>
</standard>
</inist>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Huadong Pei" sort="Huadong Pei" uniqKey="Huadong Pei" last="Huadong Pei">HUADONG PEI</name>
</noRegion>
<name sortKey="Chaomin Sun" sort="Chaomin Sun" uniqKey="Chaomin Sun" last="Chaomin Sun">CHAOMIN SUN</name>
<name sortKey="Chaomin Sun" sort="Chaomin Sun" uniqKey="Chaomin Sun" last="Chaomin Sun">CHAOMIN SUN</name>
<name sortKey="Chen Wang" sort="Chen Wang" uniqKey="Chen Wang" last="Chen Wang">CHEN WANG</name>
<name sortKey="Hua Xiang" sort="Hua Xiang" uniqKey="Hua Xiang" last="Hua Xiang">HUA XIANG</name>
<name sortKey="Huadong Pei" sort="Huadong Pei" uniqKey="Huadong Pei" last="Huadong Pei">HUADONG PEI</name>
<name sortKey="Jian Zhou" sort="Jian Zhou" uniqKey="Jian Zhou" last="Jian Zhou">JIAN ZHOU</name>
<name sortKey="Jie Ding" sort="Jie Ding" uniqKey="Jie Ding" last="Jie Ding">JIE DING</name>
<name sortKey="Jingfang Liu" sort="Jingfang Liu" uniqKey="Jingfang Liu" last="Jingfang Liu">JINGFANG LIU</name>
<name sortKey="Jingfang Liu" sort="Jingfang Liu" uniqKey="Jingfang Liu" last="Jingfang Liu">JINGFANG LIU</name>
<name sortKey="Yueping Lu" sort="Yueping Lu" uniqKey="Yueping Lu" last="Yueping Lu">YUEPING LU</name>
<name sortKey="Yun Cheng" sort="Yun Cheng" uniqKey="Yun Cheng" last="Yun Cheng">YUN CHENG</name>
</country>
</tree>
</affiliations>
</record>

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Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination

Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination

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