SrasV1, PascalFrancis, Corpus, bibRecord, 000569

Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination

Identifieur interne : 000569 ( PascalFrancis/Corpus ); précédent : 000568; suivant : 000570

Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination

Auteurs : HUADONG PEI ; JINGFANG LIU ; YUN CHENG ; CHAOMIN SUN ; CHEN WANG ; YUEPING LU ; JIE DING ; JIAN ZHOU ; HUA XIANG

Source :

Applied microbiology and biotechnology [ 0175-7598 ] ; 2005.

RBID : Pascal:06-0009448

Descripteurs français

Pascal (Inist)
- Nucléocapside, Protéine, Escherichia coli, Lactococcus lactis, Diagnostic, Coronavirus, Vaccination, Syndrome respiratoire aigu sévère, Expression génique, Protéine recombinante.

English descriptors

KwdEn :
- Coronavirus, Diagnosis, Escherichia coli, Gene expression, Lactococcus lactis, Nucleocapsid, Protein, Recombinant protein, Severe acute respiratory syndrome, Vaccination.

Abstract

The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`02`	`1`		`@1 JINGFANG LIU`
A11	`03`	`1`		`@1 YUN CHENG`
A11	`04`	`1`		`@1 CHAOMIN SUN`
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A11	`08`	`1`		`@1 JIAN ZHOU`
A11	`09`	`1`		`@1 HUA XIANG`
A14	`01`			`@1 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences @2 Beijing, 100080 @3 CHN @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 8 aut. @Z 9 aut.`
A14	`02`			`@1 Graduate school of the Chinese Academy of Sciences @2 Beijing, 100039 @3 CHN @Z 1 aut. @Z 2 aut. @Z 4 aut.`
A14	`03`			`@1 Chao-Yang Hospital Affiliated to Capital University of Medical Sciences @2 Beijing, 100020 @3 CHN @Z 5 aut. @Z 6 aut. @Z 7 aut.`
A20				`@1 220-227`
A21				`@1 2005`
A23	`01`			`@0 ENG`
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A44				`@0 0000 @1 © 2006 INIST-CNRS. All rights reserved.`
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C01	`01`		`ENG`	@0 The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.
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C03	`05`	`X`	`ENG`	`@0 Diagnosis @5 12`
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C03	`07`	`X`	`FRE`	`@0 Vaccination @5 19`
C03	`07`	`X`	`ENG`	`@0 Vaccination @5 19`
C03	`07`	`X`	`SPA`	`@0 Vacunación @5 19`
C03	`08`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 20`
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C03	`09`	`X`	`FRE`	`@0 Expression génique @5 21`
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C07	`04`	`X`	`FRE`	`@0 Micrococcales @2 NS`
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C07	`05`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
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C07	`08`	`X`	`FRE`	`@0 Virose`
C07	`08`	`X`	`ENG`	`@0 Viral disease`
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C07	`10`	`X`	`SPA`	`@0 Bacteria láctica @5 23`
N21				`@1 002`

Format Inist (serveur)

NO :	PASCAL 06-0009448 INIST
ET :	Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination
AU :	HUADONG PEI; JINGFANG LIU; YUN CHENG; CHAOMIN SUN; CHEN WANG; YUEPING LU; JIE DING; JIAN ZHOU; HUA XIANG
AF :	State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences/Beijing, 100080/Chine (1 aut., 2 aut., 3 aut., 4 aut., 8 aut., 9 aut.); Graduate school of the Chinese Academy of Sciences/Beijing, 100039/Chine (1 aut., 2 aut., 4 aut.); Chao-Yang Hospital Affiliated to Capital University of Medical Sciences/Beijing, 100020/Chine (5 aut., 6 aut., 7 aut.)
DT :	Publication en série; Niveau analytique
SO :	Applied microbiology and biotechnology; ISSN 0175-7598; Coden AMBIDG; Allemagne; Da. 2005; Vol. 68; No. 2; Pp. 220-227; Bibl. 20 ref.
LA :	Anglais
EA :	The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.
CC :	002A31D01G; 215
FD :	Nucléocapside; Protéine; Escherichia coli; Lactococcus lactis; Diagnostic; Coronavirus; Vaccination; Syndrome respiratoire aigu sévère; Expression génique; Protéine recombinante
FG :	Enterobacteriaceae; Bactérie; Streptococcaceae; Micrococcales; Coronaviridae; Nidovirales; Virus; Virose; Infection; Bactérie lactique
ED :	Nucleocapsid; Protein; Escherichia coli; Lactococcus lactis; Diagnosis; Coronavirus; Vaccination; Severe acute respiratory syndrome; Gene expression; Recombinant protein
EG :	Enterobacteriaceae; Bacteria; Streptococcaceae; Micrococcales; Coronaviridae; Nidovirales; Virus; Viral disease; Infection; Lactic acid bacteria
SD :	Nucleocápside; Proteína; Escherichia coli; Lactococcus lactis; Diagnóstico; Coronavirus; Vacunación; Síndrome respiratorio agudo severo; Expresión genética; Proteína recombinante
LO :	INIST-16771.354000131512620100
ID :	06-0009448

Links to Exploration step

Pascal:06-0009448

Le document en format XML

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</affiliation>
</author>
<author><name sortKey="Jian Zhou" sort="Jian Zhou" uniqKey="Jian Zhou" last="Jian Zhou">JIAN ZHOU</name>
<affiliation><inist:fA14 i1="01"><s1>State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences</s1>
<s2>Beijing, 100080</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
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</affiliation>
</author>
<author><name sortKey="Hua Xiang" sort="Hua Xiang" uniqKey="Hua Xiang" last="Hua Xiang">HUA XIANG</name>
<affiliation><inist:fA14 i1="01"><s1>State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences</s1>
<s2>Beijing, 100080</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
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<series><title level="j" type="main">Applied microbiology and biotechnology</title>
<title level="j" type="abbreviated">Appl. microbiol. biotechnol.</title>
<idno type="ISSN">0175-7598</idno>
<imprint><date when="2005">2005</date>
</imprint>
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<seriesStmt><title level="j" type="main">Applied microbiology and biotechnology</title>
<title level="j" type="abbreviated">Appl. microbiol. biotechnol.</title>
<idno type="ISSN">0175-7598</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Diagnosis</term>
<term>Escherichia coli</term>
<term>Gene expression</term>
<term>Lactococcus lactis</term>
<term>Nucleocapsid</term>
<term>Protein</term>
<term>Recombinant protein</term>
<term>Severe acute respiratory syndrome</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Nucléocapside</term>
<term>Protéine</term>
<term>Escherichia coli</term>
<term>Lactococcus lactis</term>
<term>Diagnostic</term>
<term>Coronavirus</term>
<term>Vaccination</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Expression génique</term>
<term>Protéine recombinante</term>
</keywords>
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<front><div type="abstract" xml:lang="en">The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.</div>
</front>
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<fA08 i1="01" i2="1" l="ENG"><s1>Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination</s1>
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<fA11 i1="01" i2="1"><s1>HUADONG PEI</s1>
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<fA11 i1="02" i2="1"><s1>JINGFANG LIU</s1>
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<fA11 i1="03" i2="1"><s1>YUN CHENG</s1>
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<fA11 i1="04" i2="1"><s1>CHAOMIN SUN</s1>
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<fA11 i1="05" i2="1"><s1>CHEN WANG</s1>
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<fA11 i1="06" i2="1"><s1>YUEPING LU</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>JIE DING</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>JIAN ZHOU</s1>
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<fA11 i1="09" i2="1"><s1>HUA XIANG</s1>
</fA11>
<fA14 i1="01"><s1>State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences</s1>
<s2>Beijing, 100080</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Graduate school of the Chinese Academy of Sciences</s1>
<s2>Beijing, 100039</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Chao-Yang Hospital Affiliated to Capital University of Medical Sciences</s1>
<s2>Beijing, 100020</s2>
<s3>CHN</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
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<fA20><s1>220-227</s1>
</fA20>
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</fA21>
<fA23 i1="01"><s0>ENG</s0>
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<s2>16771</s2>
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<fA44><s0>0000</s0>
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<fA47 i1="01" i2="1"><s0>06-0009448</s0>
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</fA60>
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</fA66>
<fC01 i1="01" l="ENG"><s0>The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A31D01G</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Nucléocapside</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Nucleocapsid</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Nucleocápside</s0>
<s5>01</s5>
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<s5>02</s5>
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<s5>02</s5>
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<fC03 i1="02" i2="X" l="SPA"><s0>Proteína</s0>
<s5>02</s5>
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<fC03 i1="03" i2="X" l="FRE"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>10</s5>
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<fC03 i1="03" i2="X" l="ENG"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>10</s5>
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<fC03 i1="03" i2="X" l="SPA"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>10</s5>
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<fC03 i1="04" i2="X" l="FRE"><s0>Lactococcus lactis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Lactococcus lactis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Lactococcus lactis</s0>
<s2>NS</s2>
<s5>11</s5>
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<fC03 i1="05" i2="X" l="FRE"><s0>Diagnostic</s0>
<s5>12</s5>
</fC03>
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<s5>12</s5>
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<s5>12</s5>
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<fC03 i1="06" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
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<fC03 i1="06" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
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<fC03 i1="07" i2="X" l="FRE"><s0>Vaccination</s0>
<s5>19</s5>
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<fC03 i1="07" i2="X" l="ENG"><s0>Vaccination</s0>
<s5>19</s5>
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<s5>19</s5>
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<fC03 i1="08" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
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<s5>20</s5>
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<fC03 i1="08" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>20</s5>
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<fC03 i1="08" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>20</s5>
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<s5>21</s5>
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<fC03 i1="09" i2="X" l="ENG"><s0>Gene expression</s0>
<s5>21</s5>
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<s5>21</s5>
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<fC03 i1="10" i2="X" l="FRE"><s0>Protéine recombinante</s0>
<s5>22</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Recombinant protein</s0>
<s5>22</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Proteína recombinante</s0>
<s5>22</s5>
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<fC07 i1="01" i2="X" l="FRE"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
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<fC07 i1="01" i2="X" l="ENG"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
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<fC07 i1="01" i2="X" l="SPA"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
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<fC07 i1="02" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Streptococcaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Streptococcaceae</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="SPA"><s0>Streptococcaceae</s0>
<s2>NS</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Micrococcales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Micrococcales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Micrococcales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
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<fC07 i1="06" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
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<fC07 i1="07" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
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<s2>NW</s2>
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<fC07 i1="07" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
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<fC07 i1="08" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Bactérie lactique</s0>
<s5>23</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Lactic acid bacteria</s0>
<s5>23</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Bacteria láctica</s0>
<s5>23</s5>
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<server><NO>PASCAL 06-0009448 INIST</NO>
<ET>Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination</ET>
<AU>HUADONG PEI; JINGFANG LIU; YUN CHENG; CHAOMIN SUN; CHEN WANG; YUEPING LU; JIE DING; JIAN ZHOU; HUA XIANG</AU>
<AF>State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences/Beijing, 100080/Chine (1 aut., 2 aut., 3 aut., 4 aut., 8 aut., 9 aut.); Graduate school of the Chinese Academy of Sciences/Beijing, 100039/Chine (1 aut., 2 aut., 4 aut.); Chao-Yang Hospital Affiliated to Capital University of Medical Sciences/Beijing, 100020/Chine (5 aut., 6 aut., 7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Applied microbiology and biotechnology; ISSN 0175-7598; Coden AMBIDG; Allemagne; Da. 2005; Vol. 68; No. 2; Pp. 220-227; Bibl. 20 ref.</SO>
<LA>Anglais</LA>
<EA>The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/ pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.</EA>
<CC>002A31D01G; 215</CC>
<FD>Nucléocapside; Protéine; Escherichia coli; Lactococcus lactis; Diagnostic; Coronavirus; Vaccination; Syndrome respiratoire aigu sévère; Expression génique; Protéine recombinante</FD>
<FG>Enterobacteriaceae; Bactérie; Streptococcaceae; Micrococcales; Coronaviridae; Nidovirales; Virus; Virose; Infection; Bactérie lactique</FG>
<ED>Nucleocapsid; Protein; Escherichia coli; Lactococcus lactis; Diagnosis; Coronavirus; Vaccination; Severe acute respiratory syndrome; Gene expression; Recombinant protein</ED>
<EG>Enterobacteriaceae; Bacteria; Streptococcaceae; Micrococcales; Coronaviridae; Nidovirales; Virus; Viral disease; Infection; Lactic acid bacteria</EG>
<SD>Nucleocápside; Proteína; Escherichia coli; Lactococcus lactis; Diagnóstico; Coronavirus; Vacunación; Síndrome respiratorio agudo severo; Expresión genética; Proteína recombinante</SD>
<LO>INIST-16771.354000131512620100</LO>
<ID>06-0009448</ID>
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Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination

Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination

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