SrasV1, Main, Exploration, bibRecord, 004E14

Identification of natural compounds with antiviral activities against SARS-associated coronavirus

Identifieur interne : 004E14 ( Main/Exploration ); précédent : 004E13; suivant : 004E15

Identification of natural compounds with antiviral activities against SARS-associated coronavirus

Source :

Antiviral research [ 0166-3542 ] ; 2005.

RBID : Pascal:05-0294893

Descripteurs français

KwdFr :
- Alcaloïdes des Amaryllidaceae (), Alcaloïdes des Amaryllidaceae (pharmacologie), Animaux, Antiviraux (), Antiviraux (pharmacologie), Cellules Vero, Effet cytopathogène viral, Humains, Lignée cellulaire, Liliaceae (), Médicaments issus de plantes chinoises (), Médicaments issus de plantes chinoises (pharmacologie), Phytothérapie, Phénanthridines (), Phénanthridines (pharmacologie), Sels de tétrazolium, Tests de sensibilité microbienne, Virus du SRAS (), Évaluation préclinique de médicament.
MESH :
- pharmacologie : Alcaloïdes des Amaryllidaceae, Antiviraux, Médicaments issus de plantes chinoises, Phénanthridines.
Pascal (Inist)
- Alcaloïdes des Amaryllidaceae, Animaux, Antiviraux, Cellules Vero, Effet cytopathogène viral, Humains, Identification, Antiviral, Lignée cellulaire, Liliaceae, Médicaments issus de plantes chinoises, Phytothérapie, Phénanthridines, Sels de tétrazolium, Tests de sensibilité microbienne, Virus du SRAS, Virus syndrome respiratoire aigu sévère, Syndrome respiratoire aigu sévère, Médicament, Criblage, Dépistage, Évaluation préclinique de médicament.
Wicri :
- topic : Médicament.

English descriptors

KwdEn :
- Amaryllidaceae Alkaloids (chemistry), Amaryllidaceae Alkaloids (pharmacology), Animals, Antiviral, Antiviral Agents (chemistry), Antiviral Agents (pharmacology), Cell Line, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Drug, Drug Evaluation, Preclinical, Drugs, Chinese Herbal (chemistry), Drugs, Chinese Herbal (pharmacology), Humans, Identification, Liliaceae (chemistry), Medical screening, Microbial Sensitivity Tests, Phenanthridines (chemistry), Phenanthridines (pharmacology), Phytotherapy, SARS Virus (drug effects), Screening, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Tetrazolium Salts, Vero Cells.
MESH :
- chemical , chemistry : Amaryllidaceae Alkaloids, Antiviral Agents, Drugs, Chinese Herbal, Phenanthridines.
- chemical , pharmacology : Amaryllidaceae Alkaloids, Antiviral Agents, Drugs, Chinese Herbal, Phenanthridines.
- chemistry : Liliaceae.
- drug effects : SARS Virus.
- Animals, Cell Line, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Drug Evaluation, Preclinical, Humans, Microbial Sensitivity Tests, Phytotherapy, Tetrazolium Salts, Vero Cells.

Abstract

More than 200 Chinese medicinal herb extracts were screened for antiviral activities against Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV) using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay for virus-induced cytopathic effect (CPE). Four of these extracts showed moderate to potent antiviral activities against SARS-CoV with 50% effective concentration (EC₅₀) ranging from 2.4 ± 0.2 to 88.2 ± 7.7 μg/ml. Out of the four, Lycoris radiata was most potent. To identify the active component, L. radiata extract was subjected to further fractionation, purification, and CPE/MTS assays. This process led to the identification of a single substance lycorine as an anti-SARS-CoV component with an EC₅₀ value of 15.7 ± 1.2 nM. This compound has a CC₅₀ value of 14980.0±912.0 nM in cytotoxicity assay and a selective index (SI) greater than 900. The results suggested that four herbal extracts and the compound lycorine are candidates for the development of new anti-SARS-CoV drugs in the treatment of SARS.

Affiliations:

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Identification of natural compounds with antiviral activities against SARS-associated coronavirus</title>
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<country>République populaire de Chine</country>
<wicri:noRegion>HD Biosciences Co.. Ltd</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jun Yu" sort="Jun Yu" uniqKey="Jun Yu" last="Jun Yu">JUN YU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Beijing Genomics Institute, Chinese Academy of Sciences</s1>
<s2>Beijing 101300</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Xiao, Pei Gen" sort="Xiao, Pei Gen" uniqKey="Xiao P" first="Pei-Gen" last="Xiao">Pei-Gen Xiao</name>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences</s1>
<s2>Beijing 100094</s2>
<s3>CHN</s3>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Rong Song" sort="Li, Rong Song" uniqKey="Li R" first="Rong-Song" last="Li">Rong-Song Li</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>HD Biosciences Co.. Ltd</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>HD Biosciences Co.. Ltd</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xuehai Tan" sort="Xuehai Tan" uniqKey="Xuehai Tan" last="Xuehai Tan">XUEHAI TAN</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Beijing Genomics Institute, Chinese Academy of Sciences</s1>
<s2>Beijing 101300</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>HD Biosciences Co.. Ltd</s1>
<s2>Shanghai 201203</s2>
<s3>CHN</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>HD Biosciences Co.. Ltd</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Antiviral research</title>
<title level="j" type="abbreviated">Antivir. res.</title>
<idno type="ISSN">0166-3542</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Antiviral research</title>
<title level="j" type="abbreviated">Antivir. res.</title>
<idno type="ISSN">0166-3542</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amaryllidaceae Alkaloids (chemistry)</term>
<term>Amaryllidaceae Alkaloids (pharmacology)</term>
<term>Animals</term>
<term>Antiviral</term>
<term>Antiviral Agents (chemistry)</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Cytopathogenic Effect, Viral</term>
<term>Drug</term>
<term>Drug Evaluation, Preclinical</term>
<term>Drugs, Chinese Herbal (chemistry)</term>
<term>Drugs, Chinese Herbal (pharmacology)</term>
<term>Humans</term>
<term>Identification</term>
<term>Liliaceae (chemistry)</term>
<term>Medical screening</term>
<term>Microbial Sensitivity Tests</term>
<term>Phenanthridines (chemistry)</term>
<term>Phenanthridines (pharmacology)</term>
<term>Phytotherapy</term>
<term>SARS Virus (drug effects)</term>
<term>Screening</term>
<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Tetrazolium Salts</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alcaloïdes des Amaryllidaceae ()</term>
<term>Alcaloïdes des Amaryllidaceae (pharmacologie)</term>
<term>Animaux</term>
<term>Antiviraux ()</term>
<term>Antiviraux (pharmacologie)</term>
<term>Cellules Vero</term>
<term>Effet cytopathogène viral</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Liliaceae ()</term>
<term>Médicaments issus de plantes chinoises ()</term>
<term>Médicaments issus de plantes chinoises (pharmacologie)</term>
<term>Phytothérapie</term>
<term>Phénanthridines ()</term>
<term>Phénanthridines (pharmacologie)</term>
<term>Sels de tétrazolium</term>
<term>Tests de sensibilité microbienne</term>
<term>Virus du SRAS ()</term>
<term>Évaluation préclinique de médicament</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Amaryllidaceae Alkaloids</term>
<term>Antiviral Agents</term>
<term>Drugs, Chinese Herbal</term>
<term>Phenanthridines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Amaryllidaceae Alkaloids</term>
<term>Antiviral Agents</term>
<term>Drugs, Chinese Herbal</term>
<term>Phenanthridines</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Liliaceae</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Alcaloïdes des Amaryllidaceae</term>
<term>Antiviraux</term>
<term>Médicaments issus de plantes chinoises</term>
<term>Phénanthridines</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Cytopathogenic Effect, Viral</term>
<term>Drug Evaluation, Preclinical</term>
<term>Humans</term>
<term>Microbial Sensitivity Tests</term>
<term>Phytotherapy</term>
<term>Tetrazolium Salts</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Alcaloïdes des Amaryllidaceae</term>
<term>Animaux</term>
<term>Antiviraux</term>
<term>Cellules Vero</term>
<term>Effet cytopathogène viral</term>
<term>Humains</term>
<term>Identification</term>
<term>Antiviral</term>
<term>Lignée cellulaire</term>
<term>Liliaceae</term>
<term>Médicaments issus de plantes chinoises</term>
<term>Phytothérapie</term>
<term>Phénanthridines</term>
<term>Sels de tétrazolium</term>
<term>Tests de sensibilité microbienne</term>
<term>Virus du SRAS</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Médicament</term>
<term>Criblage</term>
<term>Dépistage</term>
<term>Évaluation préclinique de médicament</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Médicament</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">More than 200 Chinese medicinal herb extracts were screened for antiviral activities against Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV) using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay for virus-induced cytopathic effect (CPE). Four of these extracts showed moderate to potent antiviral activities against SARS-CoV with 50% effective concentration (EC<sub>50</sub>
) ranging from 2.4 ± 0.2 to 88.2 ± 7.7 μg/ml. Out of the four, Lycoris radiata was most potent. To identify the active component, L. radiata extract was subjected to further fractionation, purification, and CPE/MTS assays. This process led to the identification of a single substance lycorine as an anti-SARS-CoV component with an EC<sub>50</sub>
 value of 15.7 ± 1.2 nM. This compound has a CC<sub>50</sub>
 value of 14980.0±912.0 nM in cytotoxicity assay and a selective index (SI) greater than 900. The results suggested that four herbal extracts and the compound lycorine are candidates for the development of new anti-SARS-CoV drugs in the treatment of SARS.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Shi You" sort="Li, Shi You" uniqKey="Li S" first="Shi-You" last="Li">Shi-You Li</name>
</noRegion>
<name sortKey="Cong Chen" sort="Cong Chen" uniqKey="Cong Chen" last="Cong Chen">CONG CHEN</name>
<name sortKey="Cong Chen" sort="Cong Chen" uniqKey="Cong Chen" last="Cong Chen">CONG CHEN</name>
<name sortKey="Guo, Hai Yan" sort="Guo, Hai Yan" uniqKey="Guo H" first="Hai-Yan" last="Guo">Hai-Yan Guo</name>
<name sortKey="Hua, Shi Neng" sort="Hua, Shi Neng" uniqKey="Hua S" first="Shi-Neng" last="Hua">Shi-Neng Hua</name>
<name sortKey="Hui Wang" sort="Hui Wang" uniqKey="Hui Wang" last="Hui Wang">HUI WANG</name>
<name sortKey="Jun Yu" sort="Jun Yu" uniqKey="Jun Yu" last="Jun Yu">JUN YU</name>
<name sortKey="Li, Rong Song" sort="Li, Rong Song" uniqKey="Li R" first="Rong-Song" last="Li">Rong-Song Li</name>
<name sortKey="Li, Shi You" sort="Li, Shi You" uniqKey="Li S" first="Shi-You" last="Li">Shi-You Li</name>
<name sortKey="Lin Wang" sort="Lin Wang" uniqKey="Lin Wang" last="Lin Wang">LIN WANG</name>
<name sortKey="Lin Wang" sort="Lin Wang" uniqKey="Lin Wang" last="Lin Wang">LIN WANG</name>
<name sortKey="Xiang Zhang" sort="Xiang Zhang" uniqKey="Xiang Zhang" last="Xiang Zhang">XIANG ZHANG</name>
<name sortKey="Xiao, Pei Gen" sort="Xiao, Pei Gen" uniqKey="Xiao P" first="Pei-Gen" last="Xiao">Pei-Gen Xiao</name>
<name sortKey="Xuehai Tan" sort="Xuehai Tan" uniqKey="Xuehai Tan" last="Xuehai Tan">XUEHAI TAN</name>
<name sortKey="Xuehai Tan" sort="Xuehai Tan" uniqKey="Xuehai Tan" last="Xuehai Tan">XUEHAI TAN</name>
<name sortKey="Zhang, Hai Qing" sort="Zhang, Hai Qing" uniqKey="Zhang H" first="Hai-Qing" last="Zhang">Hai-Qing Zhang</name>
<name sortKey="Zhang, Hai Qing" sort="Zhang, Hai Qing" uniqKey="Zhang H" first="Hai-Qing" last="Zhang">Hai-Qing Zhang</name>
</country>
</tree>
</affiliations>
</record>

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Identification of natural compounds with antiviral activities against SARS-associated coronavirus

Identification of natural compounds with antiviral activities against SARS-associated coronavirus

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