Identification of natural compounds with antiviral activities against SARS-associated coronavirus.
Identifieur interne : 000F38 ( Ncbi/Curation ); précédent : 000F37; suivant : 000F39Identification of natural compounds with antiviral activities against SARS-associated coronavirus.
Auteurs : Shi-You Li [République populaire de Chine] ; Cong Chen ; Hai-Qing Zhang ; Hai-Yan Guo ; Hui Wang ; Lin Wang ; Xiang Zhang ; Shi-Neng Hua ; Jun Yu ; Pei-Gen Xiao ; Rong-Song Li ; Xuehai TanSource :
- Antiviral research [ 0166-3542 ] ; 2005.
Descripteurs français
- KwdFr :
- Alcaloïdes des Amaryllidaceae (), Alcaloïdes des Amaryllidaceae (pharmacologie), Animaux, Antiviraux (), Antiviraux (pharmacologie), Cellules Vero, Effet cytopathogène viral, Humains, Lignée cellulaire, Liliaceae (), Médicaments issus de plantes chinoises (), Médicaments issus de plantes chinoises (pharmacologie), Phytothérapie, Phénanthridines (), Phénanthridines (pharmacologie), Sels de tétrazolium, Tests de sensibilité microbienne, Virus du SRAS (), Évaluation préclinique de médicament.
- MESH :
- pharmacologie : Alcaloïdes des Amaryllidaceae, Antiviraux, Médicaments issus de plantes chinoises, Phénanthridines.
- Alcaloïdes des Amaryllidaceae, Animaux, Antiviraux, Cellules Vero, Effet cytopathogène viral, Humains, Lignée cellulaire, Liliaceae, Médicaments issus de plantes chinoises, Phytothérapie, Phénanthridines, Sels de tétrazolium, Tests de sensibilité microbienne, Virus du SRAS, Évaluation préclinique de médicament.
English descriptors
- KwdEn :
- Amaryllidaceae Alkaloids (chemistry), Amaryllidaceae Alkaloids (pharmacology), Animals, Antiviral Agents (chemistry), Antiviral Agents (pharmacology), Cell Line, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Drug Evaluation, Preclinical, Drugs, Chinese Herbal (chemistry), Drugs, Chinese Herbal (pharmacology), Humans, Liliaceae (chemistry), Microbial Sensitivity Tests, Phenanthridines (chemistry), Phenanthridines (pharmacology), Phytotherapy, SARS Virus (drug effects), Tetrazolium Salts, Vero Cells.
- MESH :
- chemical , chemistry : Amaryllidaceae Alkaloids, Antiviral Agents, Drugs, Chinese Herbal, Phenanthridines.
- chemical , pharmacology : Amaryllidaceae Alkaloids, Antiviral Agents, Drugs, Chinese Herbal, Phenanthridines.
- chemistry : Liliaceae.
- drug effects : SARS Virus.
- Animals, Cell Line, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Drug Evaluation, Preclinical, Humans, Microbial Sensitivity Tests, Phytotherapy, Tetrazolium Salts, Vero Cells.
Abstract
More than 200 Chinese medicinal herb extracts were screened for antiviral activities against Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV) using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay for virus-induced cytopathic effect (CPE). Four of these extracts showed moderate to potent antiviral activities against SARS-CoV with 50% effective concentration (EC50) ranging from 2.4 +/- 0.2 to 88.2 +/- 7.7 microg/ml. Out of the four, Lycoris radiata was most potent. To identify the active component, L. radiata extract was subjected to further fractionation, purification, and CPE/MTS assays. This process led to the identification of a single substance lycorine as an anti-SARS-CoV component with an EC50 value of 15.7 +/- 1.2 nM. This compound has a CC50 value of 14980.0 +/- 912.0 nM in cytotoxicity assay and a selective index (SI) greater than 900. The results suggested that four herbal extracts and the compound lycorine are candidates for the development of new anti-SARS-CoV drugs in the treatment of SARS.
DOI: 10.1016/j.antiviral.2005.02.007
PubMed: 15885816
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pubmed:15885816Le document en format XML
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coronavirus.</title><author><name sortKey="Li, Shi You" sort="Li, Shi You" uniqKey="Li S" first="Shi-You" last="Li">Shi-You Li</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Cong" sort="Chen, Cong" uniqKey="Chen C" first="Cong" last="Chen">Cong Chen</name></author><author><name sortKey="Zhang, Hai Qing" sort="Zhang, Hai Qing" uniqKey="Zhang H" first="Hai-Qing" last="Zhang">Hai-Qing Zhang</name></author><author><name sortKey="Guo, Hai Yan" sort="Guo, Hai Yan" uniqKey="Guo H" first="Hai-Yan" last="Guo">Hai-Yan Guo</name></author><author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name></author><author><name sortKey="Zhang, Xiang" sort="Zhang, Xiang" uniqKey="Zhang X" first="Xiang" last="Zhang">Xiang Zhang</name></author><author><name sortKey="Hua, Shi Neng" sort="Hua, Shi Neng" uniqKey="Hua S" first="Shi-Neng" last="Hua">Shi-Neng Hua</name></author><author><name sortKey="Yu, Jun" sort="Yu, Jun" uniqKey="Yu J" first="Jun" last="Yu">Jun Yu</name></author><author><name sortKey="Xiao, Pei Gen" sort="Xiao, Pei Gen" uniqKey="Xiao P" first="Pei-Gen" last="Xiao">Pei-Gen Xiao</name></author><author><name sortKey="Li, Rong Song" sort="Li, Rong Song" uniqKey="Li R" first="Rong-Song" last="Li">Rong-Song Li</name></author><author><name sortKey="Tan, Xuehai" sort="Tan, Xuehai" uniqKey="Tan X" first="Xuehai" last="Tan">Xuehai Tan</name></author></analytic><series><title level="j">Antiviral research</title><idno 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des Amaryllidaceae ()</term><term>Alcaloïdes des Amaryllidaceae (pharmacologie)</term><term>Animaux</term><term>Antiviraux ()</term><term>Antiviraux (pharmacologie)</term><term>Cellules Vero</term><term>Effet cytopathogène viral</term><term>Humains</term><term>Lignée cellulaire</term><term>Liliaceae ()</term><term>Médicaments issus de plantes chinoises ()</term><term>Médicaments issus de plantes chinoises (pharmacologie)</term><term>Phytothérapie</term><term>Phénanthridines ()</term><term>Phénanthridines (pharmacologie)</term><term>Sels de tétrazolium</term><term>Tests de sensibilité microbienne</term><term>Virus du SRAS ()</term><term>Évaluation préclinique de médicament</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Amaryllidaceae Alkaloids</term><term>Antiviral Agents</term><term>Drugs, Chinese Herbal</term><term>Phenanthridines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Amaryllidaceae Alkaloids</term><term>Antiviral Agents</term><term>Drugs, Chinese Herbal</term><term>Phenanthridines</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Liliaceae</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Alcaloïdes des Amaryllidaceae</term><term>Antiviraux</term><term>Médicaments issus de plantes chinoises</term><term>Phénanthridines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Drug Evaluation, Preclinical</term><term>Humans</term><term>Microbial Sensitivity Tests</term><term>Phytotherapy</term><term>Tetrazolium Salts</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alcaloïdes des Amaryllidaceae</term><term>Animaux</term><term>Antiviraux</term><term>Cellules Vero</term><term>Effet cytopathogène viral</term><term>Humains</term><term>Lignée cellulaire</term><term>Liliaceae</term><term>Médicaments issus de plantes chinoises</term><term>Phytothérapie</term><term>Phénanthridines</term><term>Sels de tétrazolium</term><term>Tests de sensibilité microbienne</term><term>Virus du SRAS</term><term>Évaluation préclinique de médicament</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">More than 200 Chinese medicinal herb extracts were screened for antiviral activities against Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV) using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay for virus-induced cytopathic effect (CPE). Four of these extracts showed moderate to potent antiviral activities against SARS-CoV with 50% effective concentration (EC50) ranging from 2.4 +/- 0.2 to 88.2 +/- 7.7 microg/ml. Out of the four, Lycoris radiata was most potent. To identify the active component, L. radiata extract was subjected to further fractionation, purification, and CPE/MTS assays. This process led to the identification of a single substance lycorine as an anti-SARS-CoV component with an EC50 value of 15.7 +/- 1.2 nM. This compound has a CC50 value of 14980.0 +/- 912.0 nM in cytotoxicity assay and a selective index (SI) greater than 900. The results suggested that four herbal extracts and the compound lycorine are candidates for the development of new anti-SARS-CoV drugs in the treatment of SARS.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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