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The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: expression and functional characterization.

Identifieur interne : 004584 ( Main/Exploration ); précédent : 004583; suivant : 004585

The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: expression and functional characterization.

Auteurs : Hua Geng [République populaire de Chine] ; Yuet-Man Liu ; Wai-Sing Chan ; Anthony Wing-Ip Lo ; Deborah Mun-Yee Au ; Mary Miu-Yee Waye ; Yuan-Yuan Ho

Source :

RBID : pubmed:16310783

Descripteurs français

English descriptors

Abstract

The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.

DOI: 10.1016/j.febslet.2005.11.007
PubMed: 16310783


Affiliations:


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Le document en format XML

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<div type="abstract" xml:lang="en">The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.</div>
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