SrasV1, Main, Exploration, bibRecord, 004000

Accelerated induction of apoptosis in insect cells by baculovirus-expressed SARS-CoV membrane protein.

Identifieur interne : 004000 ( Main/Exploration ); précédent : 003F99; suivant : 004001

Accelerated induction of apoptosis in insect cells by baculovirus-expressed SARS-CoV membrane protein.

Auteurs : Chia-Wei Lai [Taïwan] ; Zun-Ren Chan ; Ding-Gang Yang ; Wen-Hsin Lo ; Yiu-Kay Lai ; Margaret Dah-Tsyr Chang ; Yu-Chen Hu

Source :

FEBS letters [ 0014-5793 ] ; 2006.

RBID : pubmed:16797548

Descripteurs français

KwdFr :
- Animaux, Apoptose, Baculoviridae (génétique), Cellules Vero, Cellules cancéreuses en culture, Cinétique, Effet cytopathogène viral, Expression des gènes, Facteurs temps, Humains, Protéines de fusion recombinantes (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Spodoptera (cytologie), Spodoptera (virologie), Survie cellulaire, Virus du SRAS (physiologie).
MESH :
- cytologie : Spodoptera.
- génétique : Baculoviridae, Protéines de la matrice virale.
- métabolisme : Protéines de fusion recombinantes, Protéines de la matrice virale.
- physiologie : Virus du SRAS.
- virologie : Spodoptera.
- Animaux, Apoptose, Cellules Vero, Cellules cancéreuses en culture, Cinétique, Effet cytopathogène viral, Expression des gènes, Facteurs temps, Humains, Survie cellulaire.

English descriptors

KwdEn :
- Animals, Apoptosis, Baculoviridae (genetics), Cell Survival, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Gene Expression, Humans, Kinetics, Recombinant Fusion Proteins (metabolism), SARS Virus (physiology), Spodoptera (cytology), Spodoptera (virology), Time Factors, Tumor Cells, Cultured, Vero Cells, Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism).
MESH :
- chemical , genetics : Viral Matrix Proteins.
- chemical , metabolism : Recombinant Fusion Proteins, Viral Matrix Proteins.
- cytology : Spodoptera.
- genetics : Baculoviridae.
- physiology : SARS Virus.
- virology : Spodoptera.
- Animals, Apoptosis, Cell Survival, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Gene Expression, Humans, Kinetics, Time Factors, Tumor Cells, Cultured, Vero Cells.

Abstract

It has been shown that severe acute respiratory syndrome-associated coronavirus (SARS-CoV) 3a and 7a proteins, but not membrane (M) protein, induce apoptosis in mammalian cells. Upon expression of SARS-CoV M protein using the baculovirus/insect cell expression system, however, we found that the expressed M protein triggered accelerated apoptosis in insect cells, as characterized by rapid cell death, elevated cytotoxicity, cell shrinkage, nuclear condensation and DNA fragmentation. Conversely, the M protein expressed in mammalian cells did not induce apoptosis. This is the first report describing the induction of apoptosis by SARS-CoV M protein in animal cells and possible implications are discussed.

DOI: 10.1016/j.febslet.2006.06.003
PubMed: 16797548

Affiliations:

Taïwan

Le document en format XML

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<term>Viral Matrix Proteins (metabolism)</term>
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<front><div type="abstract" xml:lang="en">It has been shown that severe acute respiratory syndrome-associated coronavirus (SARS-CoV) 3a and 7a proteins, but not membrane (M) protein, induce apoptosis in mammalian cells. Upon expression of SARS-CoV M protein using the baculovirus/insect cell expression system, however, we found that the expressed M protein triggered accelerated apoptosis in insect cells, as characterized by rapid cell death, elevated cytotoxicity, cell shrinkage, nuclear condensation and DNA fragmentation. Conversely, the M protein expressed in mammalian cells did not induce apoptosis. This is the first report describing the induction of apoptosis by SARS-CoV M protein in animal cells and possible implications are discussed.</div>
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Accelerated induction of apoptosis in insect cells by baculovirus-expressed SARS-CoV membrane protein.

Accelerated induction of apoptosis in insect cells by baculovirus-expressed SARS-CoV membrane protein.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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