Accelerated induction of apoptosis in insect cells by baculovirus-expressed SARS-CoV membrane protein.
Identifieur interne : 002319 ( PubMed/Checkpoint ); précédent : 002318; suivant : 002320Accelerated induction of apoptosis in insect cells by baculovirus-expressed SARS-CoV membrane protein.
Auteurs : Chia-Wei Lai [Taïwan] ; Zun-Ren Chan ; Ding-Gang Yang ; Wen-Hsin Lo ; Yiu-Kay Lai ; Margaret Dah-Tsyr Chang ; Yu-Chen HuSource :
- FEBS letters [ 0014-5793 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Apoptose, Baculoviridae (génétique), Cellules Vero, Cellules cancéreuses en culture, Cinétique, Effet cytopathogène viral, Expression des gènes, Facteurs temps, Humains, Protéines de fusion recombinantes (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Spodoptera (cytologie), Spodoptera (virologie), Survie cellulaire, Virus du SRAS (physiologie).
- MESH :
- cytologie : Spodoptera.
- génétique : Baculoviridae, Protéines de la matrice virale.
- métabolisme : Protéines de fusion recombinantes, Protéines de la matrice virale.
- physiologie : Virus du SRAS.
- virologie : Spodoptera.
- Animaux, Apoptose, Cellules Vero, Cellules cancéreuses en culture, Cinétique, Effet cytopathogène viral, Expression des gènes, Facteurs temps, Humains, Survie cellulaire.
English descriptors
- KwdEn :
- Animals, Apoptosis, Baculoviridae (genetics), Cell Survival, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Gene Expression, Humans, Kinetics, Recombinant Fusion Proteins (metabolism), SARS Virus (physiology), Spodoptera (cytology), Spodoptera (virology), Time Factors, Tumor Cells, Cultured, Vero Cells, Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism).
- MESH :
- chemical , genetics : Viral Matrix Proteins.
- chemical , metabolism : Recombinant Fusion Proteins, Viral Matrix Proteins.
- cytology : Spodoptera.
- genetics : Baculoviridae.
- physiology : SARS Virus.
- virology : Spodoptera.
- Animals, Apoptosis, Cell Survival, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Gene Expression, Humans, Kinetics, Time Factors, Tumor Cells, Cultured, Vero Cells.
Abstract
It has been shown that severe acute respiratory syndrome-associated coronavirus (SARS-CoV) 3a and 7a proteins, but not membrane (M) protein, induce apoptosis in mammalian cells. Upon expression of SARS-CoV M protein using the baculovirus/insect cell expression system, however, we found that the expressed M protein triggered accelerated apoptosis in insect cells, as characterized by rapid cell death, elevated cytotoxicity, cell shrinkage, nuclear condensation and DNA fragmentation. Conversely, the M protein expressed in mammalian cells did not induce apoptosis. This is the first report describing the induction of apoptosis by SARS-CoV M protein in animal cells and possible implications are discussed.
DOI: 10.1016/j.febslet.2006.06.003
PubMed: 16797548
Affiliations:
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Upon expression of SARS-CoV M protein using the baculovirus/insect cell expression system, however, we found that the expressed M protein triggered accelerated apoptosis in insect cells, as characterized by rapid cell death, elevated cytotoxicity, cell shrinkage, nuclear condensation and DNA fragmentation. Conversely, the M protein expressed in mammalian cells did not induce apoptosis. 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Upon expression of SARS-CoV M protein using the baculovirus/insect cell expression system, however, we found that the expressed M protein triggered accelerated apoptosis in insect cells, as characterized by rapid cell death, elevated cytotoxicity, cell shrinkage, nuclear condensation and DNA fragmentation. Conversely, the M protein expressed in mammalian cells did not induce apoptosis. This is the first report describing the induction of apoptosis by SARS-CoV M protein in animal cells and possible implications are discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lai</LastName><ForeName>Chia-Wei</ForeName><Initials>CW</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Zun-Ren</ForeName><Initials>ZR</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Ding-Gang</ForeName><Initials>DG</Initials></Author><Author ValidYN="Y"><LastName>Lo</LastName><ForeName>Wen-Hsin</ForeName><Initials>WH</Initials></Author><Author ValidYN="Y"><LastName>Lai</LastName><ForeName>Yiu-Kay</ForeName><Initials>YK</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Margaret Dah-Tsyr</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Yu-Chen</ForeName><Initials>YC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>06</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEBS Lett</MedlineTA><NlmUniqueID>0155157</NlmUniqueID><ISSNLinking>0014-5793</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014763">Viral Matrix Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" 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uniqKey="Chang M" first="Margaret Dah-Tsyr" last="Chang">Margaret Dah-Tsyr Chang</name><name sortKey="Hu, Yu Chen" sort="Hu, Yu Chen" uniqKey="Hu Y" first="Yu-Chen" last="Hu">Yu-Chen Hu</name><name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name><name sortKey="Lo, Wen Hsin" sort="Lo, Wen Hsin" uniqKey="Lo W" first="Wen-Hsin" last="Lo">Wen-Hsin Lo</name><name sortKey="Yang, Ding Gang" sort="Yang, Ding Gang" uniqKey="Yang D" first="Ding-Gang" last="Yang">Ding-Gang Yang</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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