SARS coronavirus E protein forms cation-selective ion channels.
Identifieur interne : 005132 ( Main/Curation ); précédent : 005131; suivant : 005133SARS coronavirus E protein forms cation-selective ion channels.
Auteurs : Lauren Wilson [Australie] ; Carolyn Mckinlay ; Peter Gage ; Gary EwartSource :
- Virology [ 0042-6822 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Peptide Fragments, Viral Envelope Proteins.
- chemical , physiology : Ion Channels, Viral Envelope Proteins.
- physiology : SARS Virus.
- Amino Acid Sequence, Lipid Bilayers, Membrane Potentials, Molecular Sequence Data.
Abstract
Severe Acute Respiratory Syndrome (SARS) is caused by a novel coronavirus (SARS-CoV). Coronaviruses including SARS-CoV encode an envelope (E) protein, a small, hydrophobic membrane protein. We report that, in planar lipid bilayers, synthetic peptides corresponding to the SARS-CoV E protein forms ion channels that are more permeable to monovalent cations than to monovalent anions. Affinity-purified polyclonal antibodies recognizing the N-terminal 19 residues of SARS-CoV E protein were used to establish the specificity of channel formation by inhibiting the ion currents generated in the presence of the E protein peptides.
DOI: 10.1016/j.virol.2004.09.033
PubMed: 15527857
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pubmed:15527857Le document en format XML
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<term>Molecular Sequence Data</term>
<term>Peptide Fragments (chemistry)</term>
<term>SARS Virus (physiology)</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (physiology)</term>
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<term>Données de séquences moléculaires</term>
<term>Double couche lipidique</term>
<term>Fragments peptidiques ()</term>
<term>Potentiels de membrane</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (physiologie)</term>
<term>Séquence d'acides aminés</term>
<term>Virus du SRAS (physiologie)</term>
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<term>Viral Envelope Proteins</term>
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<term>Protéines de l'enveloppe virale</term>
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<front><div type="abstract" xml:lang="en">Severe Acute Respiratory Syndrome (SARS) is caused by a novel coronavirus (SARS-CoV). Coronaviruses including SARS-CoV encode an envelope (E) protein, a small, hydrophobic membrane protein. We report that, in planar lipid bilayers, synthetic peptides corresponding to the SARS-CoV E protein forms ion channels that are more permeable to monovalent cations than to monovalent anions. Affinity-purified polyclonal antibodies recognizing the N-terminal 19 residues of SARS-CoV E protein were used to establish the specificity of channel formation by inhibiting the ion currents generated in the presence of the E protein peptides.</div>
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