A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
Identifieur interne : 004947 ( Main/Curation ); précédent : 004946; suivant : 004948A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
Auteurs : Elien Moës ; Leen Vijgen ; Els Keyaerts ; Kalina Zlateva ; Sandra Li ; Piet Maes ; Krzysztof Pyrc ; Ben Berkhout ; Lia Van Der Hoek ; Marc Van RanstSource :
- BMC Infectious Diseases [ 1471-2334 ] ; 2005.
Descripteurs français
- KwdFr :
- ARN viral (), Adolescent, Alignement de séquences, Amorces ADN (), Belgique (épidémiologie), Coronavirus (génétique), Coronavirus (isolement et purification), Données de séquences moléculaires, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Incidence, Infections de l'appareil respiratoire (virologie), Infections de l'appareil respiratoire (épidémiologie), Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Mâle, Nourrisson, Phylogénie, RT-PCR (), Saisons, Séquence consensus, Séquence nucléotidique.
- MESH :
- génétique : Coronavirus.
- isolement et purification : Coronavirus.
- virologie : Infections de l'appareil respiratoire, Infections à coronavirus.
- épidémiologie : Belgique, Infections de l'appareil respiratoire, Infections à coronavirus.
- ARN viral, Adolescent, Alignement de séquences, Amorces ADN, Données de séquences moléculaires, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Incidence, Mâle, Nourrisson, Phylogénie, RT-PCR, Saisons, Séquence consensus, Séquence nucléotidique.
- Wicri :
- geographic : Belgique.
English descriptors
- KwdEn :
- Adolescent, Base Sequence, Belgium (epidemiology), Child, Child, Preschool, Consensus Sequence, Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (epidemiology), Coronavirus Infections (virology), DNA Primers (chemistry), Female, Humans, Incidence, Infant, Male, Molecular Sequence Data, Phylogeny, RNA, Viral (chemistry), Respiratory Tract Infections (epidemiology), Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction (methods), Seasons, Sequence Alignment.
- MESH :
- chemical , chemistry : DNA Primers, RNA, Viral.
- geographic , epidemiology : Belgium.
- epidemiology : Coronavirus Infections, Respiratory Tract Infections.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Coronavirus Infections, Respiratory Tract Infections.
- Adolescent, Base Sequence, Child, Child, Preschool, Consensus Sequence, Female, Humans, Incidence, Infant, Male, Molecular Sequence Data, Phylogeny, Seasons, Sequence Alignment.
Abstract
Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.
Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.
Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.
Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.
The online version of this article (doi:10.1186/1471-2334-5-6) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2334-5-6
PubMed: 15686594
PubMed Central: 549190
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PMC:549190Le document en format XML
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<term>Coronavirus Infections (virology)</term>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.</p>
</sec>
<sec><title>Methods</title>
<p>Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.</p>
</sec>
<sec><title>Results</title>
<p>Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.</p>
</sec>
<sec><title>Conclusions</title>
<p>Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/1471-2334-5-6) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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