A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
Identifieur interne : 001403 ( Pmc/Checkpoint ); précédent : 001402; suivant : 001404A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
Auteurs : Elien Moës ; Leen Vijgen ; Els Keyaerts ; Kalina Zlateva ; Sandra Li ; Piet Maes ; Krzysztof Pyrc ; Ben Berkhout ; Lia Van Der Hoek ; Marc Van RanstSource :
- BMC Infectious Diseases [ 1471-2334 ] ; 2005.
Abstract
Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.
Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.
Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.
Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.
The online version of this article (doi:10.1186/1471-2334-5-6) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2334-5-6
PubMed: 15686594
PubMed Central: 549190
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:549190Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium</title><author><name sortKey="Moes, Elien" sort="Moes, Elien" uniqKey="Moes E" first="Elien" last="Moës">Elien Moës</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Vijgen, Leen" sort="Vijgen, Leen" uniqKey="Vijgen L" first="Leen" last="Vijgen">Leen Vijgen</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Keyaerts, Els" sort="Keyaerts, Els" uniqKey="Keyaerts E" first="Els" last="Keyaerts">Els Keyaerts</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Zlateva, Kalina" sort="Zlateva, Kalina" uniqKey="Zlateva K" first="Kalina" last="Zlateva">Kalina Zlateva</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Li, Sandra" sort="Li, Sandra" uniqKey="Li S" first="Sandra" last="Li">Sandra Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Maes, Piet" sort="Maes, Piet" uniqKey="Maes P" first="Piet" last="Maes">Piet Maes</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Pyrc, Krzysztof" sort="Pyrc, Krzysztof" uniqKey="Pyrc K" first="Krzysztof" last="Pyrc">Krzysztof Pyrc</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.7177.6</institution-id><institution-id institution-id-type="ISNI">0000000084992262</institution-id><institution>Department of Human Retrovirology, Academic Medical Center,</institution><institution>University of Amsterdam,</institution></institution-wrap>The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Berkhout, Ben" sort="Berkhout, Ben" uniqKey="Berkhout B" first="Ben" last="Berkhout">Ben Berkhout</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.7177.6</institution-id><institution-id institution-id-type="ISNI">0000000084992262</institution-id><institution>Department of Human Retrovirology, Academic Medical Center,</institution><institution>University of Amsterdam,</institution></institution-wrap>The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Der Hoek, Lia" sort="Van Der Hoek, Lia" uniqKey="Van Der Hoek L" first="Lia" last="Van Der Hoek">Lia Van Der Hoek</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.7177.6</institution-id><institution-id institution-id-type="ISNI">0000000084992262</institution-id><institution>Department of Human Retrovirology, Academic Medical Center,</institution><institution>University of Amsterdam,</institution></institution-wrap>The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Ranst, Marc" sort="Van Ranst, Marc" uniqKey="Van Ranst M" first="Marc" last="Van Ranst">Marc Van Ranst</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15686594</idno><idno type="pmc">549190</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC549190</idno><idno type="RBID">PMC:549190</idno><idno type="doi">10.1186/1471-2334-5-6</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000287</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000287</idno><idno type="wicri:Area/Pmc/Curation">000287</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000287</idno><idno type="wicri:Area/Pmc/Checkpoint">001403</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001403</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium</title><author><name sortKey="Moes, Elien" sort="Moes, Elien" uniqKey="Moes E" first="Elien" last="Moës">Elien Moës</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Vijgen, Leen" sort="Vijgen, Leen" uniqKey="Vijgen L" first="Leen" last="Vijgen">Leen Vijgen</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Keyaerts, Els" sort="Keyaerts, Els" uniqKey="Keyaerts E" first="Els" last="Keyaerts">Els Keyaerts</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Zlateva, Kalina" sort="Zlateva, Kalina" uniqKey="Zlateva K" first="Kalina" last="Zlateva">Kalina Zlateva</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Li, Sandra" sort="Li, Sandra" uniqKey="Li S" first="Sandra" last="Li">Sandra Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Maes, Piet" sort="Maes, Piet" uniqKey="Maes P" first="Piet" last="Maes">Piet Maes</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author><author><name sortKey="Pyrc, Krzysztof" sort="Pyrc, Krzysztof" uniqKey="Pyrc K" first="Krzysztof" last="Pyrc">Krzysztof Pyrc</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.7177.6</institution-id><institution-id institution-id-type="ISNI">0000000084992262</institution-id><institution>Department of Human Retrovirology, Academic Medical Center,</institution><institution>University of Amsterdam,</institution></institution-wrap>The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Berkhout, Ben" sort="Berkhout, Ben" uniqKey="Berkhout B" first="Ben" last="Berkhout">Ben Berkhout</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.7177.6</institution-id><institution-id institution-id-type="ISNI">0000000084992262</institution-id><institution>Department of Human Retrovirology, Academic Medical Center,</institution><institution>University of Amsterdam,</institution></institution-wrap>The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Der Hoek, Lia" sort="Van Der Hoek, Lia" uniqKey="Van Der Hoek L" first="Lia" last="Van Der Hoek">Lia Van Der Hoek</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.7177.6</institution-id><institution-id institution-id-type="ISNI">0000000084992262</institution-id><institution>Department of Human Retrovirology, Academic Medical Center,</institution><institution>University of Amsterdam,</institution></institution-wrap>The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Ranst, Marc" sort="Van Ranst, Marc" uniqKey="Van Ranst M" first="Marc" last="Van Ranst">Marc Van Ranst</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</nlm:aff></affiliation></author></analytic><series><title level="j">BMC Infectious Diseases</title><idno type="eISSN">1471-2334</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.</p></sec><sec><title>Methods</title><p>Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.</p></sec><sec><title>Results</title><p>Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.</p></sec><sec><title>Conclusions</title><p>Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2334-5-6) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, Mmc" uniqKey="Lai M">MMC Lai</name></author><author><name sortKey="Holmes, Kv" uniqKey="Holmes K">KV Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guy, Js" uniqKey="Guy J">JS Guy</name></author><author><name sortKey="Breslin, Jj" uniqKey="Breslin J">JJ Breslin</name></author><author><name sortKey="Breuhaus, B" uniqKey="Breuhaus B">B Breuhaus</name></author><author><name sortKey="Vivrette, S" uniqKey="Vivrette S">S Vivrette</name></author><author><name sortKey="Smith, Lg" uniqKey="Smith L">LG Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tyrrell, Daj" uniqKey="Tyrrell D">DAJ Tyrrell</name></author><author><name sortKey="Bynoe, Ml" uniqKey="Bynoe M">ML Bynoe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamre, D" uniqKey="Hamre D">D Hamre</name></author><author><name sortKey="Procknow, Jj" uniqKey="Procknow J">JJ Procknow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Almeida, Jd" uniqKey="Almeida J">JD Almeida</name></author><author><name sortKey="Tyrrell, Da" uniqKey="Tyrrell D">DA Tyrrell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Larson, He" uniqKey="Larson H">HE Larson</name></author><author><name sortKey="Reed, Se" uniqKey="Reed S">SE Reed</name></author><author><name sortKey="Tyrell, Daj" uniqKey="Tyrell D">DAJ Tyrell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S van der Werf</name></author><author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S Becker</name></author><author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H Rabenau</name></author><author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name></author><author><name sortKey="Kolesnikova, L" uniqKey="Kolesnikova L">L Kolesnikova</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author><author><name sortKey="Berger, A" uniqKey="Berger A">A Berger</name></author><author><name sortKey="Burguiere, Am" uniqKey="Burguiere A">AM Burguiere</name></author><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name></author><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M Eickmann</name></author><author><name sortKey="Escriou, N" uniqKey="Escriou N">N Escriou</name></author><author><name sortKey="Grywna, K" uniqKey="Grywna K">K Grywna</name></author><author><name sortKey="Kramme, S" uniqKey="Kramme S">S Kramme</name></author><author><name sortKey="Manuguerra, Jc" uniqKey="Manuguerra J">JC Manuguerra</name></author><author><name sortKey="Muller, S" uniqKey="Muller S">S Muller</name></author><author><name sortKey="Rickerts, V" uniqKey="Rickerts V">V Rickerts</name></author><author><name sortKey="Sturmer, M" uniqKey="Sturmer M">M Sturmer</name></author><author><name sortKey="Vieth, S" uniqKey="Vieth S">S Vieth</name></author><author><name sortKey="Klenk, Hd" uniqKey="Klenk H">HD Klenk</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Schmitz, H" uniqKey="Schmitz H">H Schmitz</name></author><author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D Erdman</name></author><author><name sortKey="Goldsmith, Cs" uniqKey="Goldsmith C">CS Goldsmith</name></author><author><name sortKey="Zaki, Sr" uniqKey="Zaki S">SR Zaki</name></author><author><name sortKey="Peret, T" uniqKey="Peret T">T Peret</name></author><author><name sortKey="Emery, S" uniqKey="Emery S">S Emery</name></author><author><name sortKey="Tong, S" uniqKey="Tong S">S Tong</name></author><author><name sortKey="Urbani, C" uniqKey="Urbani C">C Urbani</name></author><author><name sortKey="Comer, Ja" uniqKey="Comer J">JA Comer</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author><author><name sortKey="Rollin, Pe" uniqKey="Rollin P">PE Rollin</name></author><author><name sortKey="Dowell, Sf" uniqKey="Dowell S">SF Dowell</name></author><author><name sortKey="Ling, Ae" uniqKey="Ling A">AE Ling</name></author><author><name sortKey="Humphrey, Cd" uniqKey="Humphrey C">CD Humphrey</name></author><author><name sortKey="Shieh, Wj" uniqKey="Shieh W">WJ Shieh</name></author><author><name sortKey="Guarner, J" uniqKey="Guarner J">J Guarner</name></author><author><name sortKey="Paddock, Cd" uniqKey="Paddock C">CD Paddock</name></author><author><name sortKey="Rota, P" uniqKey="Rota P">P Rota</name></author><author><name sortKey="Fields, B" uniqKey="Fields B">B Fields</name></author><author><name sortKey="Derisi, J" uniqKey="Derisi J">J DeRisi</name></author><author><name sortKey="Yang, Jy" uniqKey="Yang J">JY Yang</name></author><author><name sortKey="Cox, N" uniqKey="Cox N">N Cox</name></author><author><name sortKey="Hughes, Jm" uniqKey="Hughes J">JM Hughes</name></author><author><name sortKey="Leduc, Jw" uniqKey="Leduc J">JW LeDuc</name></author><author><name sortKey="Bellini, Wj" uniqKey="Bellini W">WJ Bellini</name></author><author><name sortKey="Anderson, Lj" uniqKey="Anderson L">LJ Anderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author><author><name sortKey="Lai, St" uniqKey="Lai S">ST Lai</name></author><author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Yam, Ly" uniqKey="Yam L">LY Yam</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author><author><name sortKey="Nicholls, J" uniqKey="Nicholls J">J Nicholls</name></author><author><name sortKey="Yee, Wk" uniqKey="Yee W">WK Yee</name></author><author><name sortKey="Yan, Ww" uniqKey="Yan W">WW Yan</name></author><author><name sortKey="Cheung, Mt" uniqKey="Cheung M">MT Cheung</name></author><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Tsang, Dn" uniqKey="Tsang D">DN Tsang</name></author><author><name sortKey="Yung, Rw" uniqKey="Yung R">RW Yung</name></author><author><name sortKey="Ng, Tk" uniqKey="Ng T">TK Ng</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, Ma" uniqKey="Marra M">MA Marra</name></author><author><name sortKey="Jones, Sjm" uniqKey="Jones S">SJM Jones</name></author><author><name sortKey="Astell, Cr" uniqKey="Astell C">CR Astell</name></author><author><name sortKey="Holt, Ra" uniqKey="Holt R">RA Holt</name></author><author><name sortKey="Brooks Wilson, A" uniqKey="Brooks Wilson A">A Brooks-Wilson</name></author><author><name sortKey="Butterfield, Ysn" uniqKey="Butterfield Y">YSN Butterfield</name></author><author><name sortKey="Khattra, J" uniqKey="Khattra J">J Khattra</name></author><author><name sortKey="Asano, Jk" uniqKey="Asano J">JK Asano</name></author><author><name sortKey="Barber, Sa" uniqKey="Barber S">SA Barber</name></author><author><name sortKey="Chan, Sy" uniqKey="Chan S">SY Chan</name></author><author><name sortKey="Cloutier, A" uniqKey="Cloutier A">A Cloutier</name></author><author><name sortKey="Coughlin, Sm" uniqKey="Coughlin S">SM Coughlin</name></author><author><name sortKey="Freeman, D" uniqKey="Freeman D">D Freeman</name></author><author><name sortKey="Girn, N" uniqKey="Girn N">N Girn</name></author><author><name sortKey="Griffith, Ol" uniqKey="Griffith O">OL Griffith</name></author><author><name sortKey="Leach, Sr" uniqKey="Leach S">SR Leach</name></author><author><name sortKey="Mayo, M" uniqKey="Mayo M">M Mayo</name></author><author><name sortKey="Mcdonald, H" uniqKey="Mcdonald H">H McDonald</name></author><author><name sortKey="Montgomery, Sb" uniqKey="Montgomery S">SB Montgomery</name></author><author><name sortKey="Pandoh, Pk" uniqKey="Pandoh P">PK Pandoh</name></author><author><name sortKey="Petrescu, As" uniqKey="Petrescu A">AS Petrescu</name></author><author><name sortKey="Robertson, G" uniqKey="Robertson G">G Robertson</name></author><author><name sortKey="Schein, Je" uniqKey="Schein J">JE Schein</name></author><author><name sortKey="Siddiqui, A" uniqKey="Siddiqui A">A Siddiqui</name></author><author><name sortKey="Smailus, De" uniqKey="Smailus D">DE Smailus</name></author><author><name sortKey="Stott, Jm" uniqKey="Stott J">JM Stott</name></author><author><name sortKey="Yang, Gs" uniqKey="Yang G">GS Yang</name></author><author><name sortKey="Plummer, F" uniqKey="Plummer F">F Plummer</name></author><author><name sortKey="Andonov, A" uniqKey="Andonov A">A Andonov</name></author><author><name sortKey="Artsob, H" uniqKey="Artsob H">H Artsob</name></author><author><name sortKey="Bastien, N" uniqKey="Bastien N">N Bastien</name></author><author><name sortKey="Bernard, K" uniqKey="Bernard K">K Bernard</name></author><author><name sortKey="Booth, Tf" uniqKey="Booth T">TF Booth</name></author><author><name sortKey="Bowness, D" uniqKey="Bowness D">D Bowness</name></author><author><name sortKey="Czub, M" uniqKey="Czub M">M Czub</name></author><author><name sortKey="Drebot, M" uniqKey="Drebot M">M Drebot</name></author><author><name sortKey="Fernando, L" uniqKey="Fernando L">L Fernando</name></author><author><name sortKey="Flick, R" uniqKey="Flick R">R Flick</name></author><author><name sortKey="Garbutt, M" uniqKey="Garbutt M">M Garbutt</name></author><author><name sortKey="Gray, M" uniqKey="Gray M">M Gray</name></author><author><name sortKey="Grolla, A" uniqKey="Grolla A">A Grolla</name></author><author><name sortKey="Jones, S" uniqKey="Jones S">S Jones</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name></author><author><name sortKey="Meyers, A" uniqKey="Meyers A">A Meyers</name></author><author><name sortKey="Kabani, A" uniqKey="Kabani A">A Kabani</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Normand, S" uniqKey="Normand S">S Normand</name></author><author><name sortKey="Stroher, U" uniqKey="Stroher U">U Stroher</name></author><author><name sortKey="Tipples, Ga" uniqKey="Tipples G">GA Tipples</name></author><author><name sortKey="Tyler, S" uniqKey="Tyler S">S Tyler</name></author><author><name sortKey="Vogrig, R" uniqKey="Vogrig R">R Vogrig</name></author><author><name sortKey="Ward, D" uniqKey="Ward D">D Ward</name></author><author><name sortKey="Watson, B" uniqKey="Watson B">B Watson</name></author><author><name sortKey="Brunham, Rc" uniqKey="Brunham R">RC Brunham</name></author><author><name sortKey="Krajden, M" uniqKey="Krajden M">M Krajden</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author><author><name sortKey="Skowronski, D" uniqKey="Skowronski D">D Skowronski</name></author><author><name sortKey="Upton, C" uniqKey="Upton C">C Upton</name></author><author><name sortKey="Roper, R" uniqKey="Roper R">R Roper</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name></author><author><name sortKey="Oberste, Ms" uniqKey="Oberste M">MS Oberste</name></author><author><name sortKey="Monroe, Ss" uniqKey="Monroe S">SS Monroe</name></author><author><name sortKey="Nix, Wa" uniqKey="Nix W">WA Nix</name></author><author><name sortKey="Campagnoli, R" uniqKey="Campagnoli R">R Campagnoli</name></author><author><name sortKey="Icenogle, Jp" uniqKey="Icenogle J">JP Icenogle</name></author><author><name sortKey="Penaranda, S" uniqKey="Penaranda S">S Penaranda</name></author><author><name sortKey="Bankamp, B" uniqKey="Bankamp B">B Bankamp</name></author><author><name sortKey="Maher, K" uniqKey="Maher K">K Maher</name></author><author><name sortKey="Chen, Mh" uniqKey="Chen M">MH Chen</name></author><author><name sortKey="Tong, S" uniqKey="Tong S">S Tong</name></author><author><name sortKey="Tamin, A" uniqKey="Tamin A">A Tamin</name></author><author><name sortKey="Lowe, L" uniqKey="Lowe L">L Lowe</name></author><author><name sortKey="Frace, M" uniqKey="Frace M">M Frace</name></author><author><name sortKey="Derisi, Jl" uniqKey="Derisi J">JL Derisi</name></author><author><name sortKey="Chen, Q" uniqKey="Chen Q">Q Chen</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Erdman, Dd" uniqKey="Erdman D">DD Erdman</name></author><author><name sortKey="Peret, Tct" uniqKey="Peret T">TCT Peret</name></author><author><name sortKey="Burns, C" uniqKey="Burns C">C Burns</name></author><author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name></author><author><name sortKey="Rollin, Pe" uniqKey="Rollin P">PE Rollin</name></author><author><name sortKey="Sanchez, A" uniqKey="Sanchez A">A Sanchez</name></author><author><name sortKey="Liffick, S" uniqKey="Liffick S">S Liffick</name></author><author><name sortKey="Holloway, B" uniqKey="Holloway B">B Holloway</name></author><author><name sortKey="Limor, J" uniqKey="Limor J">J Limor</name></author><author><name sortKey="Mccaustland, K" uniqKey="Mccaustland K">K McCaustland</name></author><author><name sortKey="Olsen Rasmussen, M" uniqKey="Olsen Rasmussen M">M Olsen-Rasmussen</name></author><author><name sortKey="Fouchier, R" uniqKey="Fouchier R">R Fouchier</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name></author><author><name sortKey="Osterhaus, Adme" uniqKey="Osterhaus A">ADME Osterhaus</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Pallansch, Ma" uniqKey="Pallansch M">MA Pallansch</name></author><author><name sortKey="Anderson, Lj" uniqKey="Anderson L">LJ Anderson</name></author><author><name sortKey="Bellini, Wj" uniqKey="Bellini W">WJ Bellini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name></author><author><name sortKey="Bredenbeek, Pj" uniqKey="Bredenbeek P">PJ Bredenbeek</name></author><author><name sortKey="Dobbe, Jc" uniqKey="Dobbe J">JC Dobbe</name></author><author><name sortKey="Thiel, V" uniqKey="Thiel V">V Thiel</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name></author><author><name sortKey="Poon, Llm" uniqKey="Poon L">LLM Poon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Rozanov, M" uniqKey="Rozanov M">M Rozanov</name></author><author><name sortKey="Spaan, Wjm" uniqKey="Spaan W">WJM Spaan</name></author><author><name sortKey="Gorbalenya, Ae" uniqKey="Gorbalenya A">AE Gorbalenya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gorbalenya, Ae" uniqKey="Gorbalenya A">AE Gorbalenya</name></author><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name></author><author><name sortKey="Spaan, Wj" uniqKey="Spaan W">WJ Spaan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L van der Hoek</name></author><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K Pyrc</name></author><author><name sortKey="Jebbink, Mf" uniqKey="Jebbink M">MF Jebbink</name></author><author><name sortKey="Vermeulen Oost, W" uniqKey="Vermeulen Oost W">W Vermeulen-Oost</name></author><author><name sortKey="Berkhout, Rj" uniqKey="Berkhout R">RJ Berkhout</name></author><author><name sortKey="Wolthers, Kc" uniqKey="Wolthers K">KC Wolthers</name></author><author><name sortKey="Wertheim Van Dillen, Pm" uniqKey="Wertheim Van Dillen P">PM Wertheim-van Dillen</name></author><author><name sortKey="Kaandorp, J" uniqKey="Kaandorp J">J Kaandorp</name></author><author><name sortKey="Spaargaren, J" uniqKey="Spaargaren J">J Spaargaren</name></author><author><name sortKey="Berkhout, B" uniqKey="Berkhout B">B Berkhout</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author><author><name sortKey="Hartwig, Ng" uniqKey="Hartwig N">NG Hartwig</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Niemeyer, B" uniqKey="Niemeyer B">B Niemeyer</name></author><author><name sortKey="De Jong, Jc" uniqKey="De Jong J">JC de Jong</name></author><author><name sortKey="Simon, Jh" uniqKey="Simon J">JH Simon</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vabret, A" uniqKey="Vabret A">A Vabret</name></author><author><name sortKey="Mouthon, F" uniqKey="Mouthon F">F Mouthon</name></author><author><name sortKey="Mourez, T" uniqKey="Mourez T">T Mourez</name></author><author><name sortKey="Gouarin, S" uniqKey="Gouarin S">S Gouarin</name></author><author><name sortKey="Petitjean, J" uniqKey="Petitjean J">J Petitjean</name></author><author><name sortKey="Freymuth, F" uniqKey="Freymuth F">F Freymuth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stephensen, Cb" uniqKey="Stephensen C">CB Stephensen</name></author><author><name sortKey="Casebolt, Db" uniqKey="Casebolt D">DB Casebolt</name></author><author><name sortKey="Gangopadhyay, Nn" uniqKey="Gangopadhyay N">NN Gangopadhyay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Glezen, Wp" uniqKey="Glezen W">WP Glezen</name></author><author><name sortKey="Greenberg, Sb" uniqKey="Greenberg S">SB Greenberg</name></author><author><name sortKey="Atmar, Rl" uniqKey="Atmar R">RL Atmar</name></author><author><name sortKey="Piedra, Pa" uniqKey="Piedra P">PA Piedra</name></author><author><name sortKey="Couch, Rb" uniqKey="Couch R">RB Couch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mcintosh, K" uniqKey="Mcintosh K">K McIntosh</name></author><author><name sortKey="Chao, Rk" uniqKey="Chao R">RK Chao</name></author><author><name sortKey="Krause, He" uniqKey="Krause H">HE Krause</name></author><author><name sortKey="Wasil, R" uniqKey="Wasil R">R Wasil</name></author><author><name sortKey="Mocega, He" uniqKey="Mocega H">HE Mocega</name></author><author><name sortKey="Mufson, Ma" uniqKey="Mufson M">MA Mufson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gagneur, A" uniqKey="Gagneur A">A Gagneur</name></author><author><name sortKey="Sizun, J" uniqKey="Sizun J">J Sizun</name></author><author><name sortKey="Vallet, S" uniqKey="Vallet S">S Vallet</name></author><author><name sortKey="Legr, Mc" uniqKey="Legr M">MC Legr</name></author><author><name sortKey="Picard, B" uniqKey="Picard B">B Picard</name></author><author><name sortKey="Talbot, Pj" uniqKey="Talbot P">PJ Talbot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vabret, A" uniqKey="Vabret A">A Vabret</name></author><author><name sortKey="Mourez, T" uniqKey="Mourez T">T Mourez</name></author><author><name sortKey="Gouarin, S" uniqKey="Gouarin S">S Gouarin</name></author><author><name sortKey="Petitjean, J" uniqKey="Petitjean J">J Petitjean</name></author><author><name sortKey="Freymuth, F" uniqKey="Freymuth F">F Freymuth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pene, F" uniqKey="Pene F">F Pene</name></author><author><name sortKey="Merlat, A" uniqKey="Merlat A">A Merlat</name></author><author><name sortKey="Vabret, A" uniqKey="Vabret A">A Vabret</name></author><author><name sortKey="Rozenberg, F" uniqKey="Rozenberg F">F Rozenberg</name></author><author><name sortKey="Buzyn, A" uniqKey="Buzyn A">A Buzyn</name></author><author><name sortKey="Dreyfus, F" uniqKey="Dreyfus F">F Dreyfus</name></author><author><name sortKey="Cariou, A" uniqKey="Cariou A">A Cariou</name></author><author><name sortKey="Freymuth, F" uniqKey="Freymuth F">F Freymuth</name></author><author><name sortKey="Lebon, P" uniqKey="Lebon P">P Lebon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hendley, Jo" uniqKey="Hendley J">JO Hendley</name></author><author><name sortKey="Fishburne, Hb" uniqKey="Fishburne H">HB Fishburne</name></author><author><name sortKey="Gwaltney, Jm" uniqKey="Gwaltney J">JM Gwaltney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, Zf" uniqKey="Sun Z">ZF Sun</name></author><author><name sortKey="Meng, Xj" uniqKey="Meng X">XJ Meng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, Jd" uniqKey="Thompson J">JD Thompson</name></author><author><name sortKey="Gibson, Tj" uniqKey="Gibson T">TJ Gibson</name></author><author><name sortKey="Plewniak, F" uniqKey="Plewniak F">F Plewniak</name></author><author><name sortKey="Jeanmougin, F" uniqKey="Jeanmougin F">F Jeanmougin</name></author><author><name sortKey="Higgins, Dg" uniqKey="Higgins D">DG Higgins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nicholas, Kb" uniqKey="Nicholas K">KB Nicholas</name></author><author><name sortKey="Nicholas, Hb" uniqKey="Nicholas H">HB Nicholas</name></author><author><name sortKey="Deerfield, Dw" uniqKey="Deerfield D">DW Deerfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name></author><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name></author><author><name sortKey="Jakobsen, Ib" uniqKey="Jakobsen I">IB Jakobsen</name></author><author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Infect Dis</journal-id><journal-id journal-id-type="iso-abbrev">BMC Infect. Dis</journal-id><journal-title-group><journal-title>BMC Infectious Diseases</journal-title></journal-title-group><issn pub-type="epub">1471-2334</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15686594</article-id><article-id pub-id-type="pmc">549190</article-id><article-id pub-id-type="publisher-id">154</article-id><article-id pub-id-type="doi">10.1186/1471-2334-5-6</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Moës</surname><given-names>Elien</given-names></name><address><email>elien.moes@uz.kuleuven.ac.be</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Vijgen</surname><given-names>Leen</given-names></name><address><email>leen.vijgen@uz.kuleuven.ac.be</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Keyaerts</surname><given-names>Els</given-names></name><address><email>els.keyaerts@uz.kuleuven.ac.be</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zlateva</surname><given-names>Kalina</given-names></name><address><email>kalina.zlateva@uz.kuleuven.ac.be</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Sandra</given-names></name><address><email>sandra.li@uz.kuleuven.ac.be</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Maes</surname><given-names>Piet</given-names></name><address><email>piet.3.maes@uz.kuleuven.ac.be</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Pyrc</surname><given-names>Krzysztof</given-names></name><address><email>k.a.pyrc@amc.uva.nl</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Berkhout</surname><given-names>Ben</given-names></name><address><email>b.berkhout@amc.uva.nl</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>van der Hoek</surname><given-names>Lia</given-names></name><address><email>c.m.vanderhoek@amc.uva.nl</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Van Ranst</surname><given-names>Marc</given-names></name><address><email>marc.vanranst@uz.kuleuven.ac.be</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.5596.f</institution-id><institution-id institution-id-type="ISNI">0000000106687884</institution-id><institution>Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research,</institution><institution>University of Leuven,</institution></institution-wrap>Belgium</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.7177.6</institution-id><institution-id institution-id-type="ISNI">0000000084992262</institution-id><institution>Department of Human Retrovirology, Academic Medical Center,</institution><institution>University of Amsterdam,</institution></institution-wrap>The Netherlands</aff></contrib-group><pub-date pub-type="epub"><day>01</day><month>2</month><year>2005</year></pub-date><pub-date pub-type="pmc-release"><day>01</day><month>2</month><year>2005</year></pub-date><pub-date pub-type="collection"><year>2005</year></pub-date><volume>5</volume><elocation-id>6</elocation-id><history><date date-type="received"><day>13</day><month>10</month><year>2004</year></date><date date-type="accepted"><day>01</day><month>2</month><year>2005</year></date></history><permissions><copyright-statement>© Moës et al; licensee BioMed Central Ltd. 2005</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.</p></sec><sec><title>Methods</title><p>Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.</p></sec><sec><title>Results</title><p>Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.</p></sec><sec><title>Conclusions</title><p>Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2334-5-6) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Respiratory Syncytial Virus</kwd><kwd>Severe Acute Respiratory Syndrome</kwd><kwd>Porcine Epidemic Diarrhea Virus</kwd><kwd>Severe Acute Respiratory Syndrome</kwd><kwd>Nasopharyngeal Aspirate</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2005</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Berkhout, Ben" sort="Berkhout, Ben" uniqKey="Berkhout B" first="Ben" last="Berkhout">Ben Berkhout</name><name sortKey="Keyaerts, Els" sort="Keyaerts, Els" uniqKey="Keyaerts E" first="Els" last="Keyaerts">Els Keyaerts</name><name sortKey="Li, Sandra" sort="Li, Sandra" uniqKey="Li S" first="Sandra" last="Li">Sandra Li</name><name sortKey="Maes, Piet" sort="Maes, Piet" uniqKey="Maes P" first="Piet" last="Maes">Piet Maes</name><name sortKey="Moes, Elien" sort="Moes, Elien" uniqKey="Moes E" first="Elien" last="Moës">Elien Moës</name><name sortKey="Pyrc, Krzysztof" sort="Pyrc, Krzysztof" uniqKey="Pyrc K" first="Krzysztof" last="Pyrc">Krzysztof Pyrc</name><name sortKey="Van Der Hoek, Lia" sort="Van Der Hoek, Lia" uniqKey="Van Der Hoek L" first="Lia" last="Van Der Hoek">Lia Van Der Hoek</name><name sortKey="Van Ranst, Marc" sort="Van Ranst, Marc" uniqKey="Van Ranst M" first="Marc" last="Van Ranst">Marc Van Ranst</name><name sortKey="Vijgen, Leen" sort="Vijgen, Leen" uniqKey="Vijgen L" first="Leen" last="Vijgen">Leen Vijgen</name><name sortKey="Zlateva, Kalina" sort="Zlateva, Kalina" uniqKey="Zlateva K" first="Kalina" last="Zlateva">Kalina Zlateva</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001403 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 001403 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:549190
|texte= A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:15686594" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |