The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles
Identifieur interne : 003465 ( Main/Curation ); précédent : 003464; suivant : 003466The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles
Auteurs : Y. L. Siu [Hong Kong] ; K. T. Teoh [Hong Kong] ; J. Lo [Hong Kong] ; C. M. Chan [Hong Kong] ; F. Kien [Hong Kong] ; N. Escriou [France] ; S. W. Tsao [Hong Kong] ; J. M. Nicholls [Hong Kong] ; R. Altmeyer [Singapour] ; J. S. M. Peiris [Hong Kong] ; R. Bruzzone [Hong Kong] ; B. Nal [Hong Kong]Source :
- Journal of virology [ 0022-538X ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Assemblage viral, Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Humains, Microscopie électronique à transmission, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Virosomes (métabolisme), Virosomes (ultrastructure), Virus du SRAS (physiologie).
- MESH :
- génétique : Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Virosomes.
- physiologie : Virus du SRAS.
- Pascal (Inist)
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Coronavirus, Humans, Membrane Glycoproteins (metabolism), Microscopy, Electron, Transmission, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Protein, Release, SARS Virus (physiology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism), Virology, Virosomes (metabolism), Virosomes (ultrastructure), Virus Assembly, Virus like particle.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , metabolism : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Virosomes.
- physiology : SARS Virus.
- chemical , ultrastructure : Virosomes.
- Animals, Chlorocebus aethiops, Humans, Microscopy, Electron, Transmission, Spike Glycoprotein, Coronavirus, Vero Cells, Virus Assembly.
Abstract
The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.
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Pascal:08-0522049Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles</title>
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<series><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2008">2008</date>
</imprint>
</series>
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</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus</term>
<term>Humans</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Microscopy, Electron, Transmission</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>Protein</term>
<term>Release</term>
<term>SARS Virus (physiology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Matrix Proteins (genetics)</term>
<term>Viral Matrix Proteins (metabolism)</term>
<term>Virology</term>
<term>Virosomes (metabolism)</term>
<term>Virosomes (ultrastructure)</term>
<term>Virus Assembly</term>
<term>Virus like particle</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Assemblage viral</term>
<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Microscopie électronique à transmission</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines de la matrice virale (génétique)</term>
<term>Protéines de la matrice virale (métabolisme)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Virosomes (métabolisme)</term>
<term>Virosomes (ultrastructure)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
<term>Virosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Protéines nucléocapside</term>
<term>Virosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Virosomes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Microscopy, Electron, Transmission</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Virus Assembly</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Assemblage viral</term>
<term>Cellules Vero</term>
<term>Coronavirus</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Microscopie électronique à transmission</term>
<term>Protéine</term>
<term>Libération</term>
<term>Particule type viral</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virosomes</term>
</keywords>
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<front><div type="abstract" xml:lang="en">The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.</div>
</front>
</TEI>
<double idat="0022-538X:2008:Siu Y:the:m:e"><INIST><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles</title>
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<idno type="inist">08-0522049</idno>
<date when="2008">2008</date>
<idno type="stanalyst">PASCAL 08-0522049 INIST</idno>
<idno type="RBID">Pascal:08-0522049</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000245</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000743</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000244</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000244</idno>
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<idno type="wicri:Area/Main/Merge">003570</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles</title>
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<country>Hong Kong</country>
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</affiliation>
</author>
<author><name sortKey="Teoh, K T" sort="Teoh, K T" uniqKey="Teoh K" first="K. T." last="Teoh">K. T. Teoh</name>
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</affiliation>
</author>
<author><name sortKey="Chan, C M" sort="Chan, C M" uniqKey="Chan C" first="C. M." last="Chan">C. M. Chan</name>
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</inist:fA14>
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</affiliation>
</author>
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</placeName>
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</affiliation>
</author>
<author><name sortKey="Altmeyer, R" sort="Altmeyer, R" uniqKey="Altmeyer R" first="R." last="Altmeyer">R. Altmeyer</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>CombinatoRx-Singapore Pte, Ltd., 11 Biopolis Way</s1>
<s2>138667 Singapore</s2>
<s3>SGP</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Singapour</country>
<wicri:noRegion>138667 Singapore</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
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<s3>HKG</s3>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Microbiology, The University of Hong Kong, 21 Sassoon Road</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bruzzone, R" sort="Bruzzone, R" uniqKey="Bruzzone R" first="R." last="Bruzzone">R. Bruzzone</name>
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<country>Hong Kong</country>
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</affiliation>
</author>
<author><name sortKey="Nal, B" sort="Nal, B" uniqKey="Nal B" first="B." last="Nal">B. Nal</name>
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</author>
</analytic>
<series><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2008">2008</date>
</imprint>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Protein</term>
<term>Release</term>
<term>Severe acute respiratory syndrome</term>
<term>Virology</term>
<term>Virus like particle</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Protéine</term>
<term>Libération</term>
<term>Particule type viral</term>
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<front><div type="abstract" xml:lang="en">The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.</div>
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<author><name sortKey="Lo, J" sort="Lo, J" uniqKey="Lo J" first="J" last="Lo">J. Lo</name>
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<author><name sortKey="Chan, C M" sort="Chan, C M" uniqKey="Chan C" first="C M" last="Chan">C M Chan</name>
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<author><name sortKey="Kien, F" sort="Kien, F" uniqKey="Kien F" first="F" last="Kien">F. Kien</name>
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<author><name sortKey="Escriou, N" sort="Escriou, N" uniqKey="Escriou N" first="N" last="Escriou">N. Escriou</name>
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<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Microscopy, Electron, Transmission</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>SARS Virus (physiology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Matrix Proteins (genetics)</term>
<term>Viral Matrix Proteins (metabolism)</term>
<term>Virosomes (metabolism)</term>
<term>Virosomes (ultrastructure)</term>
<term>Virus Assembly</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Assemblage viral</term>
<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Microscopie électronique à transmission</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines de la matrice virale (génétique)</term>
<term>Protéines de la matrice virale (métabolisme)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Virosomes (métabolisme)</term>
<term>Virosomes (ultrastructure)</term>
<term>Virus du SRAS (physiologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
<term>Virosomes</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Protéines nucléocapside</term>
<term>Virosomes</term>
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<term>Chlorocebus aethiops</term>
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<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
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<front><div type="abstract" xml:lang="en">The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.</div>
</front>
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