The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles.
Identifieur interne : 001D36 ( Ncbi/Checkpoint ); précédent : 001D35; suivant : 001D37The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles.
Auteurs : Y L Siu [République populaire de Chine] ; K T Teoh ; J. Lo ; C M Chan ; F. Kien ; N. Escriou ; S W Tsao ; J M Nicholls ; R. Altmeyer ; J S M. Peiris ; R. Bruzzone ; B. NalSource :
- Journal of virology [ 1098-5514 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Assemblage viral, Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Humains, Microscopie électronique à transmission, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Virosomes (métabolisme), Virosomes (ultrastructure), Virus du SRAS (physiologie).
- MESH :
- génétique : Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Virosomes.
- physiologie : Virus du SRAS.
- ultrastructure : Animaux, Assemblage viral, Cellules Vero, Glycoprotéine de spicule des coronavirus, Humains, Microscopie électronique à transmission, Virosomes.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Humans, Membrane Glycoproteins (metabolism), Microscopy, Electron, Transmission, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism), Virosomes (metabolism), Virosomes (ultrastructure), Virus Assembly.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , metabolism : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Virosomes.
- physiology : SARS Virus.
- chemical , ultrastructure : Virosomes.
- Animals, Chlorocebus aethiops, Humans, Microscopy, Electron, Transmission, Spike Glycoprotein, Coronavirus, Vero Cells, Virus Assembly.
Abstract
The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.
DOI: 10.1128/JVI.01052-08
PubMed: 18753196
Affiliations:
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pubmed:18753196Le document en format XML
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Lo</name></author><author><name sortKey="Chan, C M" sort="Chan, C M" uniqKey="Chan C" first="C M" last="Chan">C M Chan</name></author><author><name sortKey="Kien, F" sort="Kien, F" uniqKey="Kien F" first="F" last="Kien">F. Kien</name></author><author><name sortKey="Escriou, N" sort="Escriou, N" uniqKey="Escriou N" first="N" last="Escriou">N. Escriou</name></author><author><name sortKey="Tsao, S W" sort="Tsao, S W" uniqKey="Tsao S" first="S W" last="Tsao">S W Tsao</name></author><author><name sortKey="Nicholls, J M" sort="Nicholls, J M" uniqKey="Nicholls J" first="J M" last="Nicholls">J M Nicholls</name></author><author><name sortKey="Altmeyer, R" sort="Altmeyer, R" uniqKey="Altmeyer R" first="R" last="Altmeyer">R. Altmeyer</name></author><author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name></author><author><name sortKey="Bruzzone, R" sort="Bruzzone, R" uniqKey="Bruzzone R" first="R" last="Bruzzone">R. Bruzzone</name></author><author><name sortKey="Nal, B" sort="Nal, B" uniqKey="Nal B" first="B" last="Nal">B. 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The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Altmeyer, R" sort="Altmeyer, R" uniqKey="Altmeyer R" first="R" last="Altmeyer">R. Altmeyer</name><name sortKey="Bruzzone, R" sort="Bruzzone, R" uniqKey="Bruzzone R" first="R" last="Bruzzone">R. Bruzzone</name><name sortKey="Chan, C M" sort="Chan, C M" uniqKey="Chan C" first="C M" last="Chan">C M Chan</name><name sortKey="Escriou, N" sort="Escriou, N" uniqKey="Escriou N" first="N" last="Escriou">N. Escriou</name><name sortKey="Kien, F" sort="Kien, F" uniqKey="Kien F" first="F" last="Kien">F. Kien</name><name sortKey="Lo, J" sort="Lo, J" uniqKey="Lo J" first="J" last="Lo">J. Lo</name><name sortKey="Nal, B" sort="Nal, B" uniqKey="Nal B" first="B" last="Nal">B. Nal</name><name sortKey="Nicholls, J M" sort="Nicholls, J M" uniqKey="Nicholls J" first="J M" last="Nicholls">J M Nicholls</name><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name><name sortKey="Teoh, K T" sort="Teoh, K T" uniqKey="Teoh K" first="K T" last="Teoh">K T Teoh</name><name sortKey="Tsao, S W" sort="Tsao, S W" uniqKey="Tsao S" first="S W" last="Tsao">S W Tsao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Siu, Y L" sort="Siu, Y L" uniqKey="Siu Y" first="Y L" last="Siu">Y L Siu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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