The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles
Identifieur interne : 000245 ( PascalFrancis/Corpus ); précédent : 000244; suivant : 000246The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles
Auteurs : Y. L. Siu ; K. T. Teoh ; J. Lo ; C. M. Chan ; F. Kien ; N. Escriou ; S. W. Tsao ; J. M. Nicholls ; R. Altmeyer ; J. S. M. Peiris ; R. Bruzzone ; B. NalSource :
- Journal of virology [ 0022-538X ] ; 2008.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
pA |
|
---|
Format Inist (serveur)
NO : | PASCAL 08-0522049 INIST |
---|---|
ET : | The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles |
AU : | SIU (Y. L.); TEOH (K. T.); LO (J.); CHAN (C. M.); KIEN (F.); ESCRIOU (N.); TSAO (S. W.); NICHOLLS (J. M.); ALTMEYER (R.); PEIRIS (J. S. M.); BRUZZONE (R.); NAL (B.) |
AF : | HKU-Pasteur Research Centre, 8 Sassoon Road/Hong-Kong (1 aut., 2 aut., 5 aut., 10 aut., 11 aut., 12 aut.); Department of Pathology, The University of Hong Kong, Queen Mary Hospital/Hong-Kong (3 aut., 8 aut.); Department of Microbiology, The University of Hong Kong, 21 Sassoon Road/Hong-Kong (4 aut., 10 aut.); Institut Pasteur, Unité de Génétique Moléculaire des Virus Respiratoires, URA-CNRS 3015, 25-28 Rue du Docteur Roux/75724 Paris/France (6 aut.); Department of Anatomy, The University of Hong Kong, 21 Sassoon Road/Hong-Kong (7 aut.); CombinatoRx-Singapore Pte, Ltd., 11 Biopolis Way/138667 Singapore/Singapour (9 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2008; Vol. 82; No. 22; Pp. 11318-11330; Bibl. 70 ref. |
LA : | Anglais |
EA : | The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress. |
CC : | 002A05C10 |
FD : | Coronavirus; Protéine; Libération; Particule type viral; Virologie; Syndrome respiratoire aigu sévère |
FG : | Coronaviridae; Nidovirales; Virus; Pathologie de l'appareil respiratoire; Virose; Infection; Pathologie des poumons |
ED : | Coronavirus; Protein; Release; Virus like particle; Virology; Severe acute respiratory syndrome |
EG : | Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease |
SD : | Coronavirus; Proteína; Liberación; Partícula tipo vírico; Virología; Síndrome respiratorio agudo severo |
LO : | INIST-13592.354000184310400320 |
ID : | 08-0522049 |
Links to Exploration step
Pascal:08-0522049Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles</title>
<author><name sortKey="Siu, Y L" sort="Siu, Y L" uniqKey="Siu Y" first="Y. L." last="Siu">Y. L. Siu</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Teoh, K T" sort="Teoh, K T" uniqKey="Teoh K" first="K. T." last="Teoh">K. T. Teoh</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Lo, J" sort="Lo, J" uniqKey="Lo J" first="J." last="Lo">J. Lo</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Pathology, The University of Hong Kong, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Chan, C M" sort="Chan, C M" uniqKey="Chan C" first="C. M." last="Chan">C. M. Chan</name>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, The University of Hong Kong, 21 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kien, F" sort="Kien, F" uniqKey="Kien F" first="F." last="Kien">F. Kien</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Escriou, N" sort="Escriou, N" uniqKey="Escriou N" first="N." last="Escriou">N. Escriou</name>
<affiliation><inist:fA14 i1="04"><s1>Institut Pasteur, Unité de Génétique Moléculaire des Virus Respiratoires, URA-CNRS 3015, 25-28 Rue du Docteur Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tsao, S W" sort="Tsao, S W" uniqKey="Tsao S" first="S. W." last="Tsao">S. W. Tsao</name>
<affiliation><inist:fA14 i1="05"><s1>Department of Anatomy, The University of Hong Kong, 21 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Nicholls, J M" sort="Nicholls, J M" uniqKey="Nicholls J" first="J. M." last="Nicholls">J. M. Nicholls</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Pathology, The University of Hong Kong, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Altmeyer, R" sort="Altmeyer, R" uniqKey="Altmeyer R" first="R." last="Altmeyer">R. Altmeyer</name>
<affiliation><inist:fA14 i1="06"><s1>CombinatoRx-Singapore Pte, Ltd., 11 Biopolis Way</s1>
<s2>138667 Singapore</s2>
<s3>SGP</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, The University of Hong Kong, 21 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Bruzzone, R" sort="Bruzzone, R" uniqKey="Bruzzone R" first="R." last="Bruzzone">R. Bruzzone</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Nal, B" sort="Nal, B" uniqKey="Nal B" first="B." last="Nal">B. Nal</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">08-0522049</idno>
<date when="2008">2008</date>
<idno type="stanalyst">PASCAL 08-0522049 INIST</idno>
<idno type="RBID">Pascal:08-0522049</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000245</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles</title>
<author><name sortKey="Siu, Y L" sort="Siu, Y L" uniqKey="Siu Y" first="Y. L." last="Siu">Y. L. Siu</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Teoh, K T" sort="Teoh, K T" uniqKey="Teoh K" first="K. T." last="Teoh">K. T. Teoh</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Lo, J" sort="Lo, J" uniqKey="Lo J" first="J." last="Lo">J. Lo</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Pathology, The University of Hong Kong, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Chan, C M" sort="Chan, C M" uniqKey="Chan C" first="C. M." last="Chan">C. M. Chan</name>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, The University of Hong Kong, 21 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kien, F" sort="Kien, F" uniqKey="Kien F" first="F." last="Kien">F. Kien</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Escriou, N" sort="Escriou, N" uniqKey="Escriou N" first="N." last="Escriou">N. Escriou</name>
<affiliation><inist:fA14 i1="04"><s1>Institut Pasteur, Unité de Génétique Moléculaire des Virus Respiratoires, URA-CNRS 3015, 25-28 Rue du Docteur Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tsao, S W" sort="Tsao, S W" uniqKey="Tsao S" first="S. W." last="Tsao">S. W. Tsao</name>
<affiliation><inist:fA14 i1="05"><s1>Department of Anatomy, The University of Hong Kong, 21 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Nicholls, J M" sort="Nicholls, J M" uniqKey="Nicholls J" first="J. M." last="Nicholls">J. M. Nicholls</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Pathology, The University of Hong Kong, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Altmeyer, R" sort="Altmeyer, R" uniqKey="Altmeyer R" first="R." last="Altmeyer">R. Altmeyer</name>
<affiliation><inist:fA14 i1="06"><s1>CombinatoRx-Singapore Pte, Ltd., 11 Biopolis Way</s1>
<s2>138667 Singapore</s2>
<s3>SGP</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, The University of Hong Kong, 21 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Bruzzone, R" sort="Bruzzone, R" uniqKey="Bruzzone R" first="R." last="Bruzzone">R. Bruzzone</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Nal, B" sort="Nal, B" uniqKey="Nal B" first="B." last="Nal">B. Nal</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2008">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Protein</term>
<term>Release</term>
<term>Severe acute respiratory syndrome</term>
<term>Virology</term>
<term>Virus like particle</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Protéine</term>
<term>Libération</term>
<term>Particule type viral</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-538X</s0>
</fA01>
<fA03 i2="1"><s0>J. virol.</s0>
</fA03>
<fA05><s2>82</s2>
</fA05>
<fA06><s2>22</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>SIU (Y. L.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>TEOH (K. T.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>LO (J.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>CHAN (C. M.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>KIEN (F.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>ESCRIOU (N.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>TSAO (S. W.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>NICHOLLS (J. M.)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>ALTMEYER (R.)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>PEIRIS (J. S. M.)</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>BRUZZONE (R.)</s1>
</fA11>
<fA11 i1="12" i2="1"><s1>NAL (B.)</s1>
</fA11>
<fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Pathology, The University of Hong Kong, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Microbiology, The University of Hong Kong, 21 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>4 aut.</sZ>
<sZ>10 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Institut Pasteur, Unité de Génétique Moléculaire des Virus Respiratoires, URA-CNRS 3015, 25-28 Rue du Docteur Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Department of Anatomy, The University of Hong Kong, 21 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>CombinatoRx-Singapore Pte, Ltd., 11 Biopolis Way</s1>
<s2>138667 Singapore</s2>
<s3>SGP</s3>
<sZ>9 aut.</sZ>
</fA14>
<fA20><s1>11318-11330</s1>
</fA20>
<fA21><s1>2008</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>13592</s2>
<s5>354000184310400320</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2008 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>70 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>08-0522049</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of virology</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Protéine</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Protein</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Proteína</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Libération</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Release</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Liberación</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Particule type viral</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Virus like particle</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Partícula tipo vírico</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Virologie</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Virology</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Virología</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Pathologie de l'appareil respiratoire</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Pathologie des poumons</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>343</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 08-0522049 INIST</NO>
<ET>The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles</ET>
<AU>SIU (Y. L.); TEOH (K. T.); LO (J.); CHAN (C. M.); KIEN (F.); ESCRIOU (N.); TSAO (S. W.); NICHOLLS (J. M.); ALTMEYER (R.); PEIRIS (J. S. M.); BRUZZONE (R.); NAL (B.)</AU>
<AF>HKU-Pasteur Research Centre, 8 Sassoon Road/Hong-Kong (1 aut., 2 aut., 5 aut., 10 aut., 11 aut., 12 aut.); Department of Pathology, The University of Hong Kong, Queen Mary Hospital/Hong-Kong (3 aut., 8 aut.); Department of Microbiology, The University of Hong Kong, 21 Sassoon Road/Hong-Kong (4 aut., 10 aut.); Institut Pasteur, Unité de Génétique Moléculaire des Virus Respiratoires, URA-CNRS 3015, 25-28 Rue du Docteur Roux/75724 Paris/France (6 aut.); Department of Anatomy, The University of Hong Kong, 21 Sassoon Road/Hong-Kong (7 aut.); CombinatoRx-Singapore Pte, Ltd., 11 Biopolis Way/138667 Singapore/Singapour (9 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2008; Vol. 82; No. 22; Pp. 11318-11330; Bibl. 70 ref.</SO>
<LA>Anglais</LA>
<EA>The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.</EA>
<CC>002A05C10</CC>
<FD>Coronavirus; Protéine; Libération; Particule type viral; Virologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Pathologie de l'appareil respiratoire; Virose; Infection; Pathologie des poumons</FG>
<ED>Coronavirus; Protein; Release; Virus like particle; Virology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Coronavirus; Proteína; Liberación; Partícula tipo vírico; Virología; Síndrome respiratorio agudo severo</SD>
<LO>INIST-13592.354000184310400320</LO>
<ID>08-0522049</ID>
</server>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000245 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000245 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PascalFrancis |étape= Corpus |type= RBID |clé= Pascal:08-0522049 |texte= The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |