Dual-Functional Plasmonic Photothermal Biosensors for Highly Accurate Severe Acute Respiratory Syndrome Coronavirus 2 Detection
Identifieur interne : 000711 ( 2020/Extraction ); précédent : 000710; suivant : 000712Dual-Functional Plasmonic Photothermal Biosensors for Highly Accurate Severe Acute Respiratory Syndrome Coronavirus 2 Detection
Auteurs : Guangyu Qiu [Suisse] ; Zhibo Gai [Suisse, République populaire de Chine] ; Yile Tao [Suisse] ; Jean Schmitt [Suisse] ; Gerd A. Kullak-Ublick [Suisse] ; Jing Wang [Suisse]Source :
- ACS Nano [ 1936-0851 ] ; 2020.
Abstract
The ongoing outbreak of the novel coronavirus disease (COVID-19) has spread globally
and poses a threat to public health in more than 200 countries. Reliable laboratory
diagnosis of the disease has been one of the foremost priorities for promoting public
health interventions. The routinely used reverse transcription polymerase chain reaction
(RT-PCR) is currently the reference method for COVID-19 diagnosis. However, it also
reported a number of false-positive or -negative cases, especially in the early stages
of the novel virus outbreak. In this work, a dual-functional plasmonic biosensor
combining the plasmonic photothermal (PPT) effect and localized surface plasmon
resonance (LSPR) sensing transduction provides an alternative and promising solution for
the clinical COVID-19 diagnosis. The two-dimensional gold nanoislands (AuNIs)
functionalized with complementary DNA receptors can perform a sensitive detection of the
selected sequences from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
through nucleic acid hybridization. For better sensing performance, the thermoplasmonic
heat is generated on the same AuNIs chip when illuminated at their plasmonic resonance
frequency. The localized PPT heat is capable to elevate the
Url:
DOI: 10.1021/acsnano.0c02439
PubMed: 32281785
PubMed Central: 7158889
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PMC:7158889Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Dual-Functional Plasmonic Photothermal Biosensors for Highly Accurate Severe
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<country xml:lang="fr">République populaire de Chine</country>
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<affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Environmental Engineering,<institution>ETH Zürich</institution>
, Zürich 8093,<country>Switzerland</country>
</nlm:aff>
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<country xml:lang="fr">Suisse</country>
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<author><name sortKey="Wang, Jing" sort="Wang, Jing" uniqKey="Wang J" first="Jing" last="Wang">Jing Wang</name>
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</nlm:aff>
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<series><title level="j">ACS Nano</title>
<idno type="ISSN">1936-0851</idno>
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<front><div type="abstract" xml:lang="en"><p content-type="toc-graphic"><graphic xlink:href="nn0c02439_0006" id="ab-tgr1"></graphic>
</p>
<p>The ongoing outbreak of the novel coronavirus disease (COVID-19) has spread globally
and poses a threat to public health in more than 200 countries. Reliable laboratory
diagnosis of the disease has been one of the foremost priorities for promoting public
health interventions. The routinely used reverse transcription polymerase chain reaction
(RT-PCR) is currently the reference method for COVID-19 diagnosis. However, it also
reported a number of false-positive or -negative cases, especially in the early stages
of the novel virus outbreak. In this work, a dual-functional plasmonic biosensor
combining the plasmonic photothermal (PPT) effect and localized surface plasmon
resonance (LSPR) sensing transduction provides an alternative and promising solution for
the clinical COVID-19 diagnosis. The two-dimensional gold nanoislands (AuNIs)
functionalized with complementary DNA receptors can perform a sensitive detection of the
selected sequences from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
through nucleic acid hybridization. For better sensing performance, the thermoplasmonic
heat is generated on the same AuNIs chip when illuminated at their plasmonic resonance
frequency. The localized PPT heat is capable to elevate the <italic>in situ</italic>
hybridization temperature and facilitate the accurate discrimination of two similar gene
sequences. Our dual-functional LSPR biosensor exhibits a high sensitivity toward the
selected SARS-CoV-2 sequences with a lower detection limit down to the concentration of
0.22 pM and allows precise detection of the specific target in a multigene mixture. This
study gains insight into the thermoplasmonic enhancement and its applicability in the
nucleic acid tests and viral disease diagnosis.</p>
</div>
</front>
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