SidaGhanaV1, Ncbi, Checkpoint, bibRecord, 000040

Suitability of a Rapid Immunochromatographic Test for Detection of Antibodies to Human Immunodeficiency Virus in Ghana, West Africa

Identifieur interne : 000040 ( Ncbi/Checkpoint ); précédent : 000039; suivant : 000041

Suitability of a Rapid Immunochromatographic Test for Detection of Antibodies to Human Immunodeficiency Virus in Ghana, West Africa

Auteurs : S. Aidoo ; W. K. Ampofo ; J. A. M. Brandful ; S. V. Nuvor ; J. K. Ansah ; N. Nii-Trebi ; J. S. Barnor ; F. Apeagyei ; T. Sata ; D. Ofori-Adjei ; K. Ishikawa

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2001.

RBID : PMC:88186

Descripteurs français

KwdFr :
- Anticorps anti-VIH (sang), Chromatographie, Ghana, Humains, Immunotransfert (), Infections à VIH (diagnostic), Infections à VIH (virologie), Sensibilité et spécificité, Sérodiagnostic du SIDA (), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie), VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie).
MESH :
- diagnostic : Infections à VIH.
- immunologie : VIH-1 (Virus de l'Immunodéficience Humaine de type 1), VIH-2 (Virus de l'Immunodéficience Humaine de type 2).
- sang : Anticorps anti-VIH.
- virologie : Infections à VIH.
- Chromatographie, Ghana, Humains, Immunotransfert, Sensibilité et spécificité, Sérodiagnostic du SIDA.
Wicri :
- geographic : Ghana.

English descriptors

KwdEn :
- AIDS Serodiagnosis (methods), Chromatography, Ghana, HIV Antibodies (blood), HIV Infections (diagnosis), HIV Infections (virology), HIV-1 (immunology), HIV-2 (immunology), Humans, Immunoblotting (methods), Sensitivity and Specificity.
MESH :
- chemical , blood : HIV Antibodies.
- geographic : Ghana.
- diagnosis : HIV Infections.
- immunology : HIV-1, HIV-2.
- methods : AIDS Serodiagnosis, Immunoblotting.
- virology : HIV Infections.
- Chromatography, Humans, Sensitivity and Specificity.

Abstract

In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using Ghanaian blood samples. Two hundred four serum and/or plasma specimens were tested. HIV screening was done by a particle agglutination test and confirmed by a Western blot (WB) test as the “gold standard.” The results revealed 125 HIV-seropositive AIDS patients, 75 HIV-seronegative healthy individuals, and 4 individuals for whom the HIV-1 result was indeterminate. The results obtained by the Determine HIV-1/2 assay and Diagnostic HIV SPOT (Genelabs), which is currently widely used in many districts in Ghana, were compared with those of the WB test, excluding the four HIV-1-indeterminate samples. The sensitivity of the Determine HIV-1/2 assay was 100%, compared with 98.0% for the HIV SPOT assay. The specificity was 100% for both tests. Determine HIV-1/2 is a single-step assay and was found to be rapid and easy to perform without any special equipment. It was highly sensitive and specific. The kit can be applied without electricity and water supplies, making it suitable for the detection of HIV antibodies especially in the rural areas of Ghana, West Africa.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC88186

DOI: 10.1128/JCM.39.7.2572-2575.2001
PubMed: 11427570
PubMed Central: 88186

Affiliations:

Links to Exploration step

PMC:88186

Le document en format XML

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<term>HIV Antibodies (blood)</term>
<term>HIV Infections (diagnosis)</term>
<term>HIV Infections (virology)</term>
<term>HIV-1 (immunology)</term>
<term>HIV-2 (immunology)</term>
<term>Humans</term>
<term>Immunoblotting (methods)</term>
<term>Sensitivity and Specificity</term>
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<term>Chromatographie</term>
<term>Ghana</term>
<term>Humains</term>
<term>Immunotransfert ()</term>
<term>Infections à VIH (diagnostic)</term>
<term>Infections à VIH (virologie)</term>
<term>Sensibilité et spécificité</term>
<term>Sérodiagnostic du SIDA ()</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie)</term>
<term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie)</term>
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<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>HIV Infections</term>
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<front><div type="abstract" xml:lang="en"><p>In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using Ghanaian blood samples. Two hundred four serum and/or plasma specimens were tested. HIV screening was done by a particle agglutination test and confirmed by a Western blot (WB) test as the “gold standard.” The results revealed 125 HIV-seropositive AIDS patients, 75 HIV-seronegative healthy individuals, and 4 individuals for whom the HIV-1 result was indeterminate. The results obtained by the Determine HIV-1/2 assay and Diagnostic HIV SPOT (Genelabs), which is currently widely used in many districts in Ghana, were compared with those of the WB test, excluding the four HIV-1-indeterminate samples. The sensitivity of the Determine HIV-1/2 assay was 100%, compared with 98.0% for the HIV SPOT assay. The specificity was 100% for both tests. Determine HIV-1/2 is a single-step assay and was found to be rapid and easy to perform without any special equipment. It was highly sensitive and specific. The kit can be applied without electricity and water supplies, making it suitable for the detection of HIV antibodies especially in the rural areas of Ghana, West Africa.</p>
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<name sortKey="Nii Trebi, N" sort="Nii Trebi, N" uniqKey="Nii Trebi N" first="N." last="Nii-Trebi">N. Nii-Trebi</name>
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Le SIDA au Ghana (serveur d'exploration)

Suitability of a Rapid Immunochromatographic Test for Detection of Antibodies to Human Immunodeficiency Virus in Ghana, West Africa

Suitability of a Rapid Immunochromatographic Test for Detection of Antibodies to Human Immunodeficiency Virus in Ghana, West Africa

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Le SIDA au Ghana (serveur d'exploration)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.