Suitability of a Rapid Immunochromatographic Test for Detection of Antibodies to Human Immunodeficiency Virus in Ghana, West Africa
Identifieur interne : 000040 ( Ncbi/Merge ); précédent : 000039; suivant : 000041Suitability of a Rapid Immunochromatographic Test for Detection of Antibodies to Human Immunodeficiency Virus in Ghana, West Africa
Auteurs : S. Aidoo ; W. K. Ampofo ; J. A. M. Brandful ; S. V. Nuvor ; J. K. Ansah ; N. Nii-Trebi ; J. S. Barnor ; F. Apeagyei ; T. Sata ; D. Ofori-Adjei ; K. IshikawaSource :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 2001.
Descripteurs français
- KwdFr :
- Anticorps anti-VIH (sang), Chromatographie, Ghana, Humains, Immunotransfert (), Infections à VIH (diagnostic), Infections à VIH (virologie), Sensibilité et spécificité, Sérodiagnostic du SIDA (), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie), VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (immunologie).
- MESH :
- diagnostic : Infections à VIH.
- immunologie : VIH-1 (Virus de l'Immunodéficience Humaine de type 1), VIH-2 (Virus de l'Immunodéficience Humaine de type 2).
- sang : Anticorps anti-VIH.
- virologie : Infections à VIH.
- Chromatographie, Ghana, Humains, Immunotransfert, Sensibilité et spécificité, Sérodiagnostic du SIDA.
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : HIV Antibodies.
- geographic : Ghana.
- diagnosis : HIV Infections.
- immunology : HIV-1, HIV-2.
- methods : AIDS Serodiagnosis, Immunoblotting.
- virology : HIV Infections.
- Chromatography, Humans, Sensitivity and Specificity.
Abstract
In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using Ghanaian blood samples. Two hundred four serum and/or plasma specimens were tested. HIV screening was done by a particle agglutination test and confirmed by a Western blot (WB) test as the “gold standard.” The results revealed 125 HIV-seropositive AIDS patients, 75 HIV-seronegative healthy individuals, and 4 individuals for whom the HIV-1 result was indeterminate. The results obtained by the Determine HIV-1/2 assay and Diagnostic HIV SPOT (Genelabs), which is currently widely used in many districts in Ghana, were compared with those of the WB test, excluding the four HIV-1-indeterminate samples. The sensitivity of the Determine HIV-1/2 assay was 100%, compared with 98.0% for the HIV SPOT assay. The specificity was 100% for both tests. Determine HIV-1/2 is a single-step assay and was found to be rapid and easy to perform without any special equipment. It was highly sensitive and specific. The kit can be applied without electricity and water supplies, making it suitable for the detection of HIV antibodies especially in the rural areas of Ghana, West Africa.
Url:
DOI: 10.1128/JCM.39.7.2572-2575.2001
PubMed: 11427570
PubMed Central: 88186
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PMC:88186Le document en format XML
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<term>HIV Antibodies (blood)</term>
<term>HIV Infections (diagnosis)</term>
<term>HIV Infections (virology)</term>
<term>HIV-1 (immunology)</term>
<term>HIV-2 (immunology)</term>
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<front><div type="abstract" xml:lang="en"><p>In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using Ghanaian blood samples. Two hundred four serum and/or plasma specimens were tested. HIV screening was done by a particle agglutination test and confirmed by a Western blot (WB) test as the “gold standard.” The results revealed 125 HIV-seropositive AIDS patients, 75 HIV-seronegative healthy individuals, and 4 individuals for whom the HIV-1 result was indeterminate. The results obtained by the Determine HIV-1/2 assay and Diagnostic HIV SPOT (Genelabs), which is currently widely used in many districts in Ghana, were compared with those of the WB test, excluding the four HIV-1-indeterminate samples. The sensitivity of the Determine HIV-1/2 assay was 100%, compared with 98.0% for the HIV SPOT assay. The specificity was 100% for both tests. Determine HIV-1/2 is a single-step assay and was found to be rapid and easy to perform without any special equipment. It was highly sensitive and specific. The kit can be applied without electricity and water supplies, making it suitable for the detection of HIV antibodies especially in the rural areas of Ghana, West Africa.</p>
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<front><div type="abstract" xml:lang="en"><p>In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using Ghanaian blood samples. Two hundred four serum and/or plasma specimens were tested. HIV screening was done by a particle agglutination test and confirmed by a Western blot (WB) test as the “gold standard.” The results revealed 125 HIV-seropositive AIDS patients, 75 HIV-seronegative healthy individuals, and 4 individuals for whom the HIV-1 result was indeterminate. The results obtained by the Determine HIV-1/2 assay and Diagnostic HIV SPOT (Genelabs), which is currently widely used in many districts in Ghana, were compared with those of the WB test, excluding the four HIV-1-indeterminate samples. The sensitivity of the Determine HIV-1/2 assay was 100%, compared with 98.0% for the HIV SPOT assay. The specificity was 100% for both tests. Determine HIV-1/2 is a single-step assay and was found to be rapid and easy to perform without any special equipment. It was highly sensitive and specific. The kit can be applied without electricity and water supplies, making it suitable for the detection of HIV antibodies especially in the rural areas of Ghana, West Africa.</p>
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<placeName><settlement type="city">Accra</settlement>
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<author><name sortKey="Ansah, J K" sort="Ansah, J K" uniqKey="Ansah J" first="J K" last="Ansah">J K Ansah</name>
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<author><name sortKey="Barnor, J S" sort="Barnor, J S" uniqKey="Barnor J" first="J S" last="Barnor">J S Barnor</name>
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<series><title level="j">Journal of clinical microbiology</title>
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<front><div type="abstract" xml:lang="en">In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using Ghanaian blood samples. Two hundred four serum and/or plasma specimens were tested. HIV screening was done by a particle agglutination test and confirmed by a Western blot (WB) test as the "gold standard." The results revealed 125 HIV-seropositive AIDS patients, 75 HIV-seronegative healthy individuals, and 4 individuals for whom the HIV-1 result was indeterminate. The results obtained by the Determine HIV-1/2 assay and Diagnostic HIV SPOT (Genelabs), which is currently widely used in many districts in Ghana, were compared with those of the WB test, excluding the four HIV-1-indeterminate samples. The sensitivity of the Determine HIV-1/2 assay was 100%, compared with 98.0% for the HIV SPOT assay. The specificity was 100% for both tests. Determine HIV-1/2 is a single-step assay and was found to be rapid and easy to perform without any special equipment. It was highly sensitive and specific. The kit can be applied without electricity and water supplies, making it suitable for the detection of HIV antibodies especially in the rural areas of Ghana, West Africa.</div>
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