Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).
Identifieur interne : 001571 ( Main/Merge ); précédent : 001570; suivant : 001572Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).
Auteurs : M. Kawamura [Japon] ; J. Katahira ; M. Fukasawa ; J. Sakuragi ; K. Ishikawa ; M. Nakai ; J A Mingle ; M. Osei-Kwasi ; V B Netty ; H. AkariSource :
- Virology [ 0042-6822 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN viral (génétique), Cartographie de restriction, Clonage moléculaire, Gènes pX, Gènes rev, Régulation de l'expression des gènes viraux, Répétition terminale longue du VIH, Similitude de séquences d'acides nucléiques, VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (génétique), Variation génétique.
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Viral.
- genetics : HIV-2.
- Cloning, Molecular, Gene Expression Regulation, Viral, Genes, pX, Genes, rev, Genetic Variation, HIV Long Terminal Repeat, Restriction Mapping, Sequence Homology, Nucleic Acid.
Abstract
A highly divergent HIV-2 designated as HIV-2[GH-2] was obtained from an AIDS-related complex (ARC) patient in Ghana. A full-length molecular clone of this isolate was obtained and a biologically active clone was constructed. Its restriction pattern differed from that of prototype HIV-2[GH-1] in 25 of 35 restriction sites, but was strikingly similar to a previously characterized HIV-2 isolate from a Ghanaian (HIV-2ALT). The conserved integrase region (288-bp fragment) previously displayed 95% identity with that of ALT but 17-20% divergence from the HIV-2 prototype member, and a new distinct subgroup (HIV-2b) of HIV-2 consisting of GH-2 and ALT was postulated (Miura et al. 1991.) These isolates, however, were biologically distinguishable from each other by its replication capacity in a monocyte line, U937, in which GH-2 could not grow but ALT grew well. In addition, the nucleotide sequence of the LTR of this new isolate displays 21% divergence from that of prototype HIV-2[GH-1], but the core enhancer, Sp1 binding sites and TATA box were conserved. Although the 3' half of the env gene sequence which is deleted in HIV-2ALT clone showed 27% diversity from the prototype, functional differences in the rev-responsive element were not observed.
PubMed: 1585652
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pubmed:1585652Le document en format XML
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<term>Gene Expression Regulation, Viral</term>
<term>Genes, pX</term>
<term>Genes, rev</term>
<term>Genetic Variation</term>
<term>HIV Long Terminal Repeat</term>
<term>HIV-2 (genetics)</term>
<term>Restriction Mapping</term>
<term>Sequence Homology, Nucleic Acid</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term>
<term>Cartographie de restriction</term>
<term>Clonage moléculaire</term>
<term>Gènes pX</term>
<term>Gènes rev</term>
<term>Régulation de l'expression des gènes viraux</term>
<term>Répétition terminale longue du VIH</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (génétique)</term>
<term>Variation génétique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-2</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term>
<term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2)</term>
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<keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term>
<term>Gene Expression Regulation, Viral</term>
<term>Genes, pX</term>
<term>Genes, rev</term>
<term>Genetic Variation</term>
<term>HIV Long Terminal Repeat</term>
<term>Restriction Mapping</term>
<term>Sequence Homology, Nucleic Acid</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term>
<term>Clonage moléculaire</term>
<term>Gènes pX</term>
<term>Gènes rev</term>
<term>Régulation de l'expression des gènes viraux</term>
<term>Répétition terminale longue du VIH</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Variation génétique</term>
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<front><div type="abstract" xml:lang="en">A highly divergent HIV-2 designated as HIV-2[GH-2] was obtained from an AIDS-related complex (ARC) patient in Ghana. A full-length molecular clone of this isolate was obtained and a biologically active clone was constructed. Its restriction pattern differed from that of prototype HIV-2[GH-1] in 25 of 35 restriction sites, but was strikingly similar to a previously characterized HIV-2 isolate from a Ghanaian (HIV-2ALT). The conserved integrase region (288-bp fragment) previously displayed 95% identity with that of ALT but 17-20% divergence from the HIV-2 prototype member, and a new distinct subgroup (HIV-2b) of HIV-2 consisting of GH-2 and ALT was postulated (Miura et al. 1991.) These isolates, however, were biologically distinguishable from each other by its replication capacity in a monocyte line, U937, in which GH-2 could not grow but ALT grew well. In addition, the nucleotide sequence of the LTR of this new isolate displays 21% divergence from that of prototype HIV-2[GH-1], but the core enhancer, Sp1 binding sites and TATA box were conserved. Although the 3' half of the env gene sequence which is deleted in HIV-2ALT clone showed 27% diversity from the prototype, functional differences in the rev-responsive element were not observed.</div>
</front>
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