Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).
Identifieur interne : 000168 ( Ncbi/Merge ); précédent : 000167; suivant : 000169Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).
Auteurs : M. Kawamura [Japon] ; J. Katahira ; M. Fukasawa ; J. Sakuragi ; K. Ishikawa ; M. Nakai ; J A Mingle ; M. Osei-Kwasi ; V B Netty ; H. AkariSource :
- Virology [ 0042-6822 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN viral (génétique), Cartographie de restriction, Clonage moléculaire, Gènes pX, Gènes rev, Régulation de l'expression des gènes viraux, Répétition terminale longue du VIH, Similitude de séquences d'acides nucléiques, VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (génétique), Variation génétique.
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Viral.
- genetics : HIV-2.
- Cloning, Molecular, Gene Expression Regulation, Viral, Genes, pX, Genes, rev, Genetic Variation, HIV Long Terminal Repeat, Restriction Mapping, Sequence Homology, Nucleic Acid.
Abstract
A highly divergent HIV-2 designated as HIV-2[GH-2] was obtained from an AIDS-related complex (ARC) patient in Ghana. A full-length molecular clone of this isolate was obtained and a biologically active clone was constructed. Its restriction pattern differed from that of prototype HIV-2[GH-1] in 25 of 35 restriction sites, but was strikingly similar to a previously characterized HIV-2 isolate from a Ghanaian (HIV-2ALT). The conserved integrase region (288-bp fragment) previously displayed 95% identity with that of ALT but 17-20% divergence from the HIV-2 prototype member, and a new distinct subgroup (HIV-2b) of HIV-2 consisting of GH-2 and ALT was postulated (Miura et al. 1991.) These isolates, however, were biologically distinguishable from each other by its replication capacity in a monocyte line, U937, in which GH-2 could not grow but ALT grew well. In addition, the nucleotide sequence of the LTR of this new isolate displays 21% divergence from that of prototype HIV-2[GH-1], but the core enhancer, Sp1 binding sites and TATA box were conserved. Although the 3' half of the env gene sequence which is deleted in HIV-2ALT clone showed 27% diversity from the prototype, functional differences in the rev-responsive element were not observed.
PubMed: 1585652
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000801
- to stream PubMed, to step Curation: 000801
- to stream PubMed, to step Checkpoint: 000801
Links to Exploration step
pubmed:1585652Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).</title><author><name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M" last="Kawamura">M. Kawamura</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Virus Research, Kyoto University, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Virus Research, Kyoto University</wicri:regionArea><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Katahira, J" sort="Katahira, J" uniqKey="Katahira J" first="J" last="Katahira">J. Katahira</name></author><author><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M" last="Fukasawa">M. Fukasawa</name></author><author><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J" last="Sakuragi">J. Sakuragi</name></author><author><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name></author><author><name sortKey="Nakai, M" sort="Nakai, M" uniqKey="Nakai M" first="M" last="Nakai">M. Nakai</name></author><author><name sortKey="Mingle, J A" sort="Mingle, J A" uniqKey="Mingle J" first="J A" last="Mingle">J A Mingle</name></author><author><name sortKey="Osei Kwasi, M" sort="Osei Kwasi, M" uniqKey="Osei Kwasi M" first="M" last="Osei-Kwasi">M. Osei-Kwasi</name></author><author><name sortKey="Netty, V B" sort="Netty, V B" uniqKey="Netty V" first="V B" last="Netty">V B Netty</name></author><author><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H" last="Akari">H. Akari</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1585652</idno><idno type="pmid">1585652</idno><idno type="wicri:Area/PubMed/Corpus">000801</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000801</idno><idno type="wicri:Area/PubMed/Curation">000801</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000801</idno><idno type="wicri:Area/PubMed/Checkpoint">000801</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000801</idno><idno type="wicri:Area/Ncbi/Merge">000168</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).</title><author><name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M" last="Kawamura">M. Kawamura</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Virus Research, Kyoto University, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Virus Research, Kyoto University</wicri:regionArea><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Katahira, J" sort="Katahira, J" uniqKey="Katahira J" first="J" last="Katahira">J. Katahira</name></author><author><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M" last="Fukasawa">M. Fukasawa</name></author><author><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J" last="Sakuragi">J. Sakuragi</name></author><author><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name></author><author><name sortKey="Nakai, M" sort="Nakai, M" uniqKey="Nakai M" first="M" last="Nakai">M. Nakai</name></author><author><name sortKey="Mingle, J A" sort="Mingle, J A" uniqKey="Mingle J" first="J A" last="Mingle">J A Mingle</name></author><author><name sortKey="Osei Kwasi, M" sort="Osei Kwasi, M" uniqKey="Osei Kwasi M" first="M" last="Osei-Kwasi">M. Osei-Kwasi</name></author><author><name sortKey="Netty, V B" sort="Netty, V B" uniqKey="Netty V" first="V B" last="Netty">V B Netty</name></author><author><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H" last="Akari">H. Akari</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular</term><term>DNA, Viral (genetics)</term><term>Gene Expression Regulation, Viral</term><term>Genes, pX</term><term>Genes, rev</term><term>Genetic Variation</term><term>HIV Long Terminal Repeat</term><term>HIV-2 (genetics)</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>Cartographie de restriction</term><term>Clonage moléculaire</term><term>Gènes pX</term><term>Gènes rev</term><term>Régulation de l'expression des gènes viraux</term><term>Répétition terminale longue du VIH</term><term>Similitude de séquences d'acides nucléiques</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (génétique)</term><term>Variation génétique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-2</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Gene Expression Regulation, Viral</term><term>Genes, pX</term><term>Genes, rev</term><term>Genetic Variation</term><term>HIV Long Terminal Repeat</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term><term>Clonage moléculaire</term><term>Gènes pX</term><term>Gènes rev</term><term>Régulation de l'expression des gènes viraux</term><term>Répétition terminale longue du VIH</term><term>Similitude de séquences d'acides nucléiques</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A highly divergent HIV-2 designated as HIV-2[GH-2] was obtained from an AIDS-related complex (ARC) patient in Ghana. A full-length molecular clone of this isolate was obtained and a biologically active clone was constructed. Its restriction pattern differed from that of prototype HIV-2[GH-1] in 25 of 35 restriction sites, but was strikingly similar to a previously characterized HIV-2 isolate from a Ghanaian (HIV-2ALT). The conserved integrase region (288-bp fragment) previously displayed 95% identity with that of ALT but 17-20% divergence from the HIV-2 prototype member, and a new distinct subgroup (HIV-2b) of HIV-2 consisting of GH-2 and ALT was postulated (Miura et al. 1991.) These isolates, however, were biologically distinguishable from each other by its replication capacity in a monocyte line, U937, in which GH-2 could not grow but ALT grew well. In addition, the nucleotide sequence of the LTR of this new isolate displays 21% divergence from that of prototype HIV-2[GH-1], but the core enhancer, Sp1 binding sites and TATA box were conserved. Although the 3' half of the env gene sequence which is deleted in HIV-2ALT clone showed 27% diversity from the prototype, functional differences in the rev-responsive element were not observed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1585652</PMID><DateCreated><Year>1992</Year><Month>06</Month><Day>17</Day></DateCreated><DateCompleted><Year>1992</Year><Month>06</Month><Day>17</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0042-6822</ISSN><JournalIssue CitedMedium="Print"><Volume>188</Volume><Issue>2</Issue><PubDate><Year>1992</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Virology</Title><ISOAbbreviation>Virology</ISOAbbreviation></Journal><ArticleTitle>Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).</ArticleTitle><Pagination><MedlinePgn>850-3</MedlinePgn></Pagination><Abstract><AbstractText>A highly divergent HIV-2 designated as HIV-2[GH-2] was obtained from an AIDS-related complex (ARC) patient in Ghana. A full-length molecular clone of this isolate was obtained and a biologically active clone was constructed. Its restriction pattern differed from that of prototype HIV-2[GH-1] in 25 of 35 restriction sites, but was strikingly similar to a previously characterized HIV-2 isolate from a Ghanaian (HIV-2ALT). The conserved integrase region (288-bp fragment) previously displayed 95% identity with that of ALT but 17-20% divergence from the HIV-2 prototype member, and a new distinct subgroup (HIV-2b) of HIV-2 consisting of GH-2 and ALT was postulated (Miura et al. 1991.) These isolates, however, were biologically distinguishable from each other by its replication capacity in a monocyte line, U937, in which GH-2 could not grow but ALT grew well. In addition, the nucleotide sequence of the LTR of this new isolate displays 21% divergence from that of prototype HIV-2[GH-1], but the core enhancer, Sp1 binding sites and TATA box were conserved. Although the 3' half of the env gene sequence which is deleted in HIV-2ALT clone showed 27% diversity from the prototype, functional differences in the rev-responsive element were not observed.</AbstractText></Abstract><AuthorList CompleteYN="N"><Author ValidYN="Y"><LastName>Kawamura</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute for Virus Research, Kyoto University, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Katahira</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Fukasawa</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Sakuragi</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ishikawa</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Nakai</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Mingle</LastName><ForeName>J A</ForeName><Initials>JA</Initials></Author><Author ValidYN="Y"><LastName>Osei-Kwasi</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Netty</LastName><ForeName>V B</ForeName><Initials>VB</Initials></Author><Author ValidYN="Y"><LastName>Akari</LastName><ForeName>H</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virology</MedlineTA><NlmUniqueID>0110674</NlmUniqueID><ISSNLinking>0042-6822</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CitationSubset>X</CitationSubset><GeneSymbolList><GeneSymbol>rev</GeneSymbol><GeneSymbol>rex</GeneSymbol></GeneSymbolList><MeshHeadingList><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015967" MajorTopicYN="Y">Gene Expression Regulation, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016355" MajorTopicYN="N">Genes, pX</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015757" MajorTopicYN="N">Genes, rev</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016325" MajorTopicYN="N">HIV Long Terminal Repeat</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015498" MajorTopicYN="N">HIV-2</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1585652</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><noCountry><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H" last="Akari">H. Akari</name><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M" last="Fukasawa">M. Fukasawa</name><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name><name sortKey="Katahira, J" sort="Katahira, J" uniqKey="Katahira J" first="J" last="Katahira">J. Katahira</name><name sortKey="Mingle, J A" sort="Mingle, J A" uniqKey="Mingle J" first="J A" last="Mingle">J A Mingle</name><name sortKey="Nakai, M" sort="Nakai, M" uniqKey="Nakai M" first="M" last="Nakai">M. Nakai</name><name sortKey="Netty, V B" sort="Netty, V B" uniqKey="Netty V" first="V B" last="Netty">V B Netty</name><name sortKey="Osei Kwasi, M" sort="Osei Kwasi, M" uniqKey="Osei Kwasi M" first="M" last="Osei-Kwasi">M. Osei-Kwasi</name><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J" last="Sakuragi">J. Sakuragi</name></noCountry><country name="Japon"><region name="Région du Kansai"><name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M" last="Kawamura">M. Kawamura</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000168 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000168 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= SidaGhanaV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:1585652
|texte= Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:1585652" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a SidaGhanaV1
| This area was generated with Dilib version V0.6.31. |  |